تاثير نانوذرات Ag، Cu و ZnO بر بيان TNF-α در موش‌های Balb/C آلوده به استافيلوكوكوس اورئوس
محورهای موضوعی : میکروب شناسی مولکولی
یگانه غلامی
1
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بهناز اسفندیاری
2
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جواد آراسته
3
1 - گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران.
2 - گروه زیست شناسی، واحد اسلامشهر، دانشگاه آزاد اسلامی، اسلامشهر، ایران.
3 - دانشکده علوم پایه، گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد تهران مرکزی.
کلید واژه: استافیلوکوکوس اورئوس, MRSA, TNF-α, نانوذرات, MIC, MBC.,
چکیده مقاله :
سابقه و هدف: مقاومت به آنتی‌بیوتیک‌ها، به‌ویژه در استافیلوکوکوس اورئوس مقاوم به متی‌سیلین (MRSA)، یک چالش جهانی است. نانوذرات (NPs) مانند نقره (Ag)، مس (Cu) و اکسید روی (ZnO) پتانسیل ضدباکتریایی دارند. این مطالعه اثربخشی این نانوذرات را علیه MRSA و تأثیرشان بر بیان ژن TNF-α در موش‌های Balb/c بررسی کرد.
مواد و روش‌ها: ۶۳ موش به ۹ گروه (۷تایی) تقسیم شدند. گروه‌های ۵ تا ۹ با MRSA آلوده و سپس با نانوذرات Ag (15.625 mg/L) ، Cu (62.5 mg/L)، Znoوانکومایسین یا محلول نمکی درمان شدند. پس از ۱ و ۵ روز، بیان TNF-α در طحال با Real-Time PCR سنجش شد. همچنین MIC و MBC نانوذرات تعیین گردید.
یافته‌ها: نانوذرات Ag و Cu اثرات بازدارندگی و باکتریکشی داشتند، درحالی‌که ZnO بی‌اثر بود. در روز ۵، بیان TNF-α در گروه وانکومایسین به‌طور معنیداری افزایش یافت و پس از آن نانوذرات Ag، Cu و ZnO بیشترین اثر را نشان دادند. در روز ۱، تنها وانکومایسین، Ag و Cu بیان TNF-α را افزایش دادند.
نتیجه‌گیری: نانوذرات Ag بیشترین تأثیر را بر بیان TNF-α داشتند، که نشان‌دهنده پتانسیل ضدباکتریایی برتر آن‌هاست. این نتایج از کاربرد نانوذرات به‌عنوان جایگزین یا مکمل آنتی‌بیوتیک‌ها در عفونت‌های MRSA حمایت می‌کند.
Background and Objective: Antibiotic resistance, especially in methicillin-resistant Staphylococcus aureus (MRSA), is a global challenge. Nanoparticles (NPs) such as silver (Ag), copper (Cu), and zinc oxide (ZnO) have shown antibacterial potential. This study aimed to evaluate the effectiveness of these nanoparticles against MRSA and their impact on TNF-α gene expression in Balb/c mice.
Materials and Methods: Sixty-three mice were divided into nine groups of seven. Groups 5 to 9 were infected with MRSA and then treated with Ag (15.625 mg/L), Cu (62.5 mg/L), ZnO nanoparticles, vancomycin, or saline solution. TNF-α expression in the spleen was measured by Real-Time PCR on days 1 and 5. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the nanoparticles were also determined.
Results: Ag and Cu nanoparticles showed inhibitory and bactericidal effects, while ZnO was ineffective. On day 5, TNF-α expression significantly increased in the vancomycin group, followed by Ag, Cu, and ZnO groups. On day 1, only vancomycin, Ag, and Cu nanoparticles increased TNF-α expression.
Conclusion: These findings indicate that Ag NPs, through modulation of TNF-α expression in Balb/c mice, may serve as promising antibacterial agents warranting further translational studies.
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