حذف زیستی کروم توسط سودوموناس پلکوگلوسی سیدا و نانوذرات سیلیسی مزوپور از خاک های آلوده به نفت
محورهای موضوعی : زیست فناوری میکروبی
مهدی شهریاری نور
1
,
شقایق طالب سربازی
2
,
فاتن دیوسر
3
1 - گروه میکروبیولوژی- دانشکده علوم پایه- دانشگاه آزاد اسلامی واحد رشت
2 - گروه میکروبیولوژی- دانشکده علوم پایه- دانشگاه آزاد اسلامی واحد رشت
3 - گروه شیمی، دانشگاه پیام نور، تهران، ایران
کلید واژه: کروم, آلودگی نفتی, حذف زیستی, سودوموناس پلکوگلوسیسیدا, نانوذرات سیلیسی مزوپور,
چکیده مقاله :
سابقه و هدف: حضور فلزات سنگین سمی و سرطان زا همچون کروم در فاضلاب صنعتی، یک آلودگی مهم برای خاک های کشاورزی و منابع طبیعی آب محسوب می شود. هدف از این مطالعه ارزیابی حذف کروم با یک جدایه باکتریایی از خاک آلوده در مقایسه با نانوذرات سیلیسی مزوپوری می باشد.مواد و روش ها: در این پژوهش به منظورجداسازی باکتری تجزیه کننده کروم، نمونه برداری از خاک های مناطق مختلف نفتی ساحل کیاشهر صورت گرفت. شناسایی مقدماتی سویه‌های جداشده بر اساس آزمون های بیوشمیایی و سپس شناسایی مولکولی باکتری با توالی یابی 16S rRNA انجام شد. حذف کروم در شرایط آزمایشگاه با سویه باکتریایی و نانوذرات بطور جداگانه با اسپکترومتر جذب اتمی (AAS) بررسی شد.یافته ها: باکتریتجزیه کننده کروم براساس تجزیه و تحلیل 16S rRNA و همولوژی ٩٩% ، سودوموناس پلکوگلوسی سیدا شناسایی شد. حذف کروم توسط نانو ذرات سیلیسی مزوپور در غلظت ٣٠٠ میکروگرم در میلی لیتر بیشتر از سویه جدا شده باکتری بوده و میزان حذف را تا ٧٥ درصد نشان داد. اما با افزایش غلظت کروم در محیط به میزان بیش از ٦٠٠ میکروگرم در میلی لیتر ، توانایی حذف کروم توسط باکتری نسبت به نانو ذرات سیلیسی مزوپور، به میزان ٨٠ درصد به ٧۲ درصد عملکرد بهتری نشان داد.نتیجه گیری: نتایج نشان داد که جدایهسودوموناس پلکوگلوسی سیدا و نانوذرات می توانند برای حذف کروم از خاک ها و آب های آلوده استفاده شوند. همچنین به منظور به منظور افزایش کارایی حذف کروم، استفاده هم زمان از باکتری حذف کننده کروم و نانو جاذب استفاده پیشنهاد می شود.
Background & Objectives: The presence of cytotoxic and carcinogenic heavy metals such as Chromium in industrial wastewater is an important pollution for agricultural soils and natural water sources. The aim of study was to evaluate chromium removal with a bacterium isolate form contaminated soil compared to mesoporous silica nanoparticles. Materials and methods: In this study, for isolate chromium decomposing bacteria, soil were sampled from different of contaminated beach of Kiashahr. Preliminary identification of the isolated strains was done based on biochemical tests and then molecular identification of bacteria by 16SrRNA sequencing. Cr removal was evaluated with resistant strain and nanoparticles by Atomic absorption spectrometry (AAS), individually. Results: Chromium-degrading bacteria were identified based on 16SrRNA analysis and 99% homology of Pseudomonas pelicoglucida. Removal of chromium by mesoporous silica nanoparticles at a concentration of 300 μg / ml was higher than the isolated strain of Pseudomonas pelicoglucida and showed a removal rate of up to 75%. However, by increasing the concentration of chromium by more than 600 micrograms per milliliter, the ability of bacteria to remove chromium from mesoporous silica nanoparticles showed better results by 80% to 72% chromium removal. Conclusion: Results indicated Pseudomonas pelicoglucida and nanoparticles can be used to remove chromium from contaminated soils and waters. It is also recommended to use chromium-removing bacteria and nano-adsorbents to remove chromium at the same time to increase the efficiency of chromium removal.
Rohr A, Crump K. Inhalation cancer risk assessment of hexavalent chromium based on
updated mortality for Painesville chromate production workers. Journal of Exposure Science
and Environmental Epidemiology. 2016; 26(2):224-231.
2. arokhnesh , Mahvi AH, Jamali . Carcinogenic and Non-Carcinogenic Risk Assessment of
Chromium in Drinking Water Sources: Birjand, Iran. Research Journal of Environmental
Toxicology. 2016; 10(3):166-171.
3. Wang , Mandal AK, Saito H, Pulliam J , Lee E , Ke J, Lu J, Ding S, Li L, Shelton
BJ, Tucker T, Evers BM, hang , Shi . Arsenic and chromium in drinking water promote
tumorigenesis in a mouse colitis-associated colorectal cancer model and the potential
mechanism is ROS-mediated Wnt/beta-catenin signaling pathway. Toxicology and applied
pharmacology. 2012; 262(1):11-21.
4. Mitra S, Sarkar A, Sen S. Removal of chromium from industrial effluents using
nanotechnology: a review. Nanotechnology for Environmental Engineering. 2017; 2(11):
1-14.
5. Taieban SMR, Torabi E, Najafpoor AA, Alidadi H, ezoli MA. Survey of Biosorption
Chromium and Cadmium from industrial effluent, by using agricultural waste material. Navid
No. 2012; 16(58): 1-14.
6. Joutey NT, Sayel H, Bahafid W, El hachtouli N. Mechanisms of hexavalent chromium
resistance and removal by microorganisms. Reviews of environmental contamination and
toxicology. 2015; 233: 45-69.
7. Elahi A, Rehman A. Multiple metal resistance and Cr 6+ reduction by bacterium,
taphylococcus sciuri A-HS1, isolated from untreated tannery effluent. Journal of King Saud
University - Science. 2018; 31(4): 1005-1013.
8. hao R, Wang B, Cai QT, Li , Liu M, Hu D, Beiguo D, Wang J, an C. Bioremediation of
Hexavalent Chromium Pollution by porosarcina saromensis M52 Isolated from Offshore
Sediments in iamen, China. Biomedical and environmental sciences. 2016; 29(2):127-136.
9. Tamindzija D, Chromikova , Spaic A, Barak I, Bernier-Latmani R, Radnovic D. Chromate
tolerance and removal of bacterial strains isolated from uncontaminated and
chromium-polluted environments. World journal of microbiology and biotechnology. 2019;
35(4):55-72.
10. Kholghi N, Amani H , Malek Mahmoodi S , Alireza Amiri A. The removal of heavy metals
(Ni, Cr, Cd) from soil contamination with crude oil using rhamanolipid biosurfactant. Journal
of Microbial World. 2019; 12(1): 62-72.
11. Meybodi SM, , Khorasani H. Biosorption of chromium by Pseudomonas sp. isolated from oil
contaminated soils of Khuzestan. Biological Journal of Microorganism. 2015; 4(14): 101-110
12. Raja CE, Anbazhagan A, Sadasivam selvam . Isolation and characterization of a metal
resistant Pseudomonas aeruginosa strain. World Journal Microbiology and Biotechnology.
2006; 2(2): 577- 585.
13. Meybodi SM, Khorasani H. Biosorption of chromium by Pseudomonas sp. isolated from oil
contaminated soils of Khuzestan. Biological Journal of Microorganism. 2014; 4(14):
101-110.
14. Wa ao , Dongyang Li, Hong ou. unctional Characterization and genomic analysis of the
Chlorantraniliprole-Degrading Strain Pseudomonas sp. W13. 2019; 6(4): 106-118.
15. Ariapad A, anjanchi MA, Arvand M. Efficient removal of anionic surfactant using partial
template-containing MCM-41. Desalination. 2012; 284: 142–149.
16. enil CK, Mohan , Lakshmanaperumalsamy P, erima MB. Optimization of
Chromium Removal by the Indigenous Bacterium Bacillus spp. REP02 Using the Response
Surface Methodology. International Scholarly Research Network. 2011.
17. Kumar S, Stecher , Li M, Knyaz C, Tamura K. ME A : Molecular Evolutionary enetics
Analysis across computing platforms. Molecular Biology and Evolution. 2018; 35:
1547-1549.
18. Abatenh E B, Tsegaye , Wassie M. Application of microorganisms in bioremediation.
Journal of Environmental Microbiology. 2017; 1(1):2-9.
19. Raghuraman T, Jerome eoffrey C, Suriyanarayanan S, Thatheyus J. Chromium Removal by
Using Chosen Pseudomonads. American Journal of Environmental Protection. 2013; 1(1):
14-6.
20. Kaur H, Kumar A. Bioremediation of hexavalent chromium in wastewater effluent by
Pseudomonas putida (MTCC 102). International Journal of Research In Earth &
Environmental Sciences 2014; 1(4):18-24.
21. Poornima K, karthik L, Swadhini SP, Mythili S, Sathiavelu A. Degradation of Chromium by
Using a Novel Strains of Pseudomonas Species. Journal of Microbial and Biochemical
Technology. 2010; 2(4): 95-99.
22. Balamurugan D, Udayasooriyan C, Kamaladevi B. Chromium ( I) reduction by Pseudomonas
putida and Bacillus subtilis isolated from contaminated soils. International journal of
environmental scinces. 2014; 5(3):522-529.
23. Choi K, Lee S, Park JO, Park J-A, Cho S-H, Lee S , et al. Chromium removal from aqueous
solution by a PEI-silica nanocomposite. Scientific Reports. 2018; 8(1):1438-1448.
24. Liu W, ang L, u S, Chen , Liu B, Li , Jiang C. Efficient removal of hexavalent
chromium from water by an adsorption reduction mechanism with sandwiched
nanocomposites. RSC Advances. 2018; 8:15087-15093.
25. Magner E. Immobilization of enzymes on mesoporous silicate materials. Chemical society
review 2013; 42(15): 6213-6222.
_||_
Rohr A, Crump K. Inhalation cancer risk assessment of hexavalent chromium based on
updated mortality for Painesville chromate production workers. Journal of Exposure Science
and Environmental Epidemiology. 2016; 26(2):224-231.
2. arokhnesh , Mahvi AH, Jamali . Carcinogenic and Non-Carcinogenic Risk Assessment of
Chromium in Drinking Water Sources: Birjand, Iran. Research Journal of Environmental
Toxicology. 2016; 10(3):166-171.
3. Wang , Mandal AK, Saito H, Pulliam J , Lee E , Ke J, Lu J, Ding S, Li L, Shelton
BJ, Tucker T, Evers BM, hang , Shi . Arsenic and chromium in drinking water promote
tumorigenesis in a mouse colitis-associated colorectal cancer model and the potential
mechanism is ROS-mediated Wnt/beta-catenin signaling pathway. Toxicology and applied
pharmacology. 2012; 262(1):11-21.
4. Mitra S, Sarkar A, Sen S. Removal of chromium from industrial effluents using
nanotechnology: a review. Nanotechnology for Environmental Engineering. 2017; 2(11):
1-14.
5. Taieban SMR, Torabi E, Najafpoor AA, Alidadi H, ezoli MA. Survey of Biosorption
Chromium and Cadmium from industrial effluent, by using agricultural waste material. Navid
No. 2012; 16(58): 1-14.
6. Joutey NT, Sayel H, Bahafid W, El hachtouli N. Mechanisms of hexavalent chromium
resistance and removal by microorganisms. Reviews of environmental contamination and
toxicology. 2015; 233: 45-69.
7. Elahi A, Rehman A. Multiple metal resistance and Cr 6+ reduction by bacterium,
taphylococcus sciuri A-HS1, isolated from untreated tannery effluent. Journal of King Saud
University - Science. 2018; 31(4): 1005-1013.
8. hao R, Wang B, Cai QT, Li , Liu M, Hu D, Beiguo D, Wang J, an C. Bioremediation of
Hexavalent Chromium Pollution by porosarcina saromensis M52 Isolated from Offshore
Sediments in iamen, China. Biomedical and environmental sciences. 2016; 29(2):127-136.
9. Tamindzija D, Chromikova , Spaic A, Barak I, Bernier-Latmani R, Radnovic D. Chromate
tolerance and removal of bacterial strains isolated from uncontaminated and
chromium-polluted environments. World journal of microbiology and biotechnology. 2019;
35(4):55-72.
10. Kholghi N, Amani H , Malek Mahmoodi S , Alireza Amiri A. The removal of heavy metals
(Ni, Cr, Cd) from soil contamination with crude oil using rhamanolipid biosurfactant. Journal
of Microbial World. 2019; 12(1): 62-72.
11. Meybodi SM, , Khorasani H. Biosorption of chromium by Pseudomonas sp. isolated from oil
contaminated soils of Khuzestan. Biological Journal of Microorganism. 2015; 4(14): 101-110
12. Raja CE, Anbazhagan A, Sadasivam selvam . Isolation and characterization of a metal
resistant Pseudomonas aeruginosa strain. World Journal Microbiology and Biotechnology.
2006; 2(2): 577- 585.
13. Meybodi SM, Khorasani H. Biosorption of chromium by Pseudomonas sp. isolated from oil
contaminated soils of Khuzestan. Biological Journal of Microorganism. 2014; 4(14):
101-110.
14. Wa ao , Dongyang Li, Hong ou. unctional Characterization and genomic analysis of the
Chlorantraniliprole-Degrading Strain Pseudomonas sp. W13. 2019; 6(4): 106-118.
15. Ariapad A, anjanchi MA, Arvand M. Efficient removal of anionic surfactant using partial
template-containing MCM-41. Desalination. 2012; 284: 142–149.
16. enil CK, Mohan , Lakshmanaperumalsamy P, erima MB. Optimization of
Chromium Removal by the Indigenous Bacterium Bacillus spp. REP02 Using the Response
Surface Methodology. International Scholarly Research Network. 2011.
17. Kumar S, Stecher , Li M, Knyaz C, Tamura K. ME A : Molecular Evolutionary enetics
Analysis across computing platforms. Molecular Biology and Evolution. 2018; 35:
1547-1549.
18. Abatenh E B, Tsegaye , Wassie M. Application of microorganisms in bioremediation.
Journal of Environmental Microbiology. 2017; 1(1):2-9.
19. Raghuraman T, Jerome eoffrey C, Suriyanarayanan S, Thatheyus J. Chromium Removal by
Using Chosen Pseudomonads. American Journal of Environmental Protection. 2013; 1(1):
14-6.
20. Kaur H, Kumar A. Bioremediation of hexavalent chromium in wastewater effluent by
Pseudomonas putida (MTCC 102). International Journal of Research In Earth &
Environmental Sciences 2014; 1(4):18-24.
21. Poornima K, karthik L, Swadhini SP, Mythili S, Sathiavelu A. Degradation of Chromium by
Using a Novel Strains of Pseudomonas Species. Journal of Microbial and Biochemical
Technology. 2010; 2(4): 95-99.
22. Balamurugan D, Udayasooriyan C, Kamaladevi B. Chromium ( I) reduction by Pseudomonas
putida and Bacillus subtilis isolated from contaminated soils. International journal of
environmental scinces. 2014; 5(3):522-529.
23. Choi K, Lee S, Park JO, Park J-A, Cho S-H, Lee S , et al. Chromium removal from aqueous
solution by a PEI-silica nanocomposite. Scientific Reports. 2018; 8(1):1438-1448.
24. Liu W, ang L, u S, Chen , Liu B, Li , Jiang C. Efficient removal of hexavalent
chromium from water by an adsorption reduction mechanism with sandwiched
nanocomposites. RSC Advances. 2018; 8:15087-15093.
25. Magner E. Immobilization of enzymes on mesoporous silicate materials. Chemical society
review 2013; 42(15): 6213-6222.