Novel BCP-Bioactive Glass-Akermanite/PCL Composite Scaffold: Physical and Mechanical Behavior, and in Vitro Bioactivity

Subject Areas : Bio Materials

Ebrahim karamian ¹ , shakiba saghirzadeh darki ²

1 - Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran
2 - Advanced Materials Research Center, Department of Materials Engineering, Najafabad Branch, Islamic Azad University, Najafabad, Iran

Received: 2020-08-03 Accepted : 2021-03-08 Published : 2020-09-01

Keywords: Composite, Bioactivity, Akermanite, bioactive glass,

Abstract :

In the present study, biphasic calcium phosphate (BCP)-bioactive glass-akermanite/PCL composite scaffold with high porosity (80%) was successfully fabricated using polymeric sponge replication and dip–coating methods. The composite was characterized via X-ray diffraction (XRD) and scanning electron microscopy (SEM). In addition, physical and mechanical properties of the composite were evaluated using porosity and compressive strength measurements. In vitro bioactivity of the composite was carried out using simulated body fluid solution (SBF). Results showed that PCL, as a polymeric coating, incremented the mechanical strength of ceramic scaffolds from 0.97 to 1.9MPa, confirming its fundamental role in recovering the mechanical properties of scaffolds in bone tissue engineering. Bioactivity evaluation resulted in developing an apatite–like layer on the surface of the scaffolds, which verified the proper bioactivity of the scaffolds. The results showed that this novel composite scaffold has the potential to be applied as a temporary substrate for bone tissue engineering.

References:

[1] E.M. Christenson, k.S. Aseth, J.J.P. Jeroen, V. D. Beucken, C.K. Chan, B. Ercan, J.A. Jansen, C.T. Laurencin, W.J. Li, R. Murugan, L.S. Nair, S. Ramakrishna, R.S. Tuan, T.J. Webster, A.G. Mikos, “Nanobiomaterials  application in orthopedics”, Journal of Orthopedic Research, Vol.25, pp.11-22, 2006.  

[2] I. Seiler, J. Johnson, “Iliac crest autogenous bone grafting: donor site complications”, Journal of South Orthopedic Association, Vol.9, pp.91-97, 2000.

[3] D. Hickey, B. Ercan, L. Sun, T. Webster, “Adding MgO nanoparticles to hydroxyapatite – PLLA nanocomposites for improved bone tissue engineering application”, Journal of Acta Biomaterialia, Vol.14, pp.175 – 184, 2014.

[4] V. Guarino, F. Causa, I. Ambrosio, “Bioactive scaffolds for bone and ligament tissue”, Journal of Expert Review of Medical Devices, Vol.4, pp.405-418, 2007.

[5] G. E. Rutkowski, C. A. Miller, S. K. Mallapragada, “Processing of polymer scaffolds: Solvent casting method of tissue engineering”, San Diego, Academic Press, 2002.

[6] K. Whang, K. E. Healy, “Methods in tissue engineering processing of polymer scaffolds freeze – drying”, San Diego, Academic Press, 2002.

[7] F. Schuth, K. S. W. Sing, J. Weitkamp, “Handbook of porous solids”, Wiley – VCH, 2002.

[8] D. Bellucci, A. Sola, V. Cannillo, “A revised replication method for bioceramic scaffolds”, Bioceramics Ashdin Publishing Bioceramics Development and Applications, Vol.1, 2011.

[9] I. Sopyan, M. Mel, S. Ramesh, K.A. Khalid, “Porous hydroxyapatite for artificial bone applications”, Journal of Science Technology of Advanced Materials, Vol.8, pp.116 – 123, 2008.

[10] J.R. Jones, L.M. Ehrenfried, L.L. Hench, “Optimizing bioactive glass scaffolds for bone tissue engineering”, Journal of Biomaterials, Vol.27, pp.964 – 973, 2006.

[11] R.K. Nalla, J.H. Kinnej, R.O., Ritchi, “Mechanistic fracture criteria for the failure of human cortical bone”, Journal of Nature Materials, Vol.2, pp.164-168, 2003.

[12] M. Parvizifard, S. Karbasi, H. Salehi, S. Soleymani Eil Bakhtiari, Evaluation of physical, mechanical and biological properties of biogalss/titania scaffold coated with poly(3-hydroxybutyrate)-chitosan for bone tissue engineering, Mat. Tech: Adv.Perform.Mat., http://doi.org/10.1080/10667857.2019.1658169.

 [13] S.A. Mali, K.C. Nune, R.D.K. Misra, Biomimetic nanostructured hydroxyapatite coatings on metallic implant materials, Mat. Tech: Adv. Perform. Mat. 31 (2016) 1-9.

[14] C. Bao, M. Chang, L. Qin, Y. Fan, E.Y. Teo, D. Sandikin, M. Choolani, J. K.Y. Chan, Effects of tricalcium phosphate in polycaprolactone scaffold for mesenchymal stem cell-based bone tissue engineering, Mat. Tech: Adv. Perform. Mat. 34 (2019) 361-367.

[15] S. Keikhaei, Z. Mohammadalizadeh, S. Karbasi, A. Salimi, Evaluation of the effects of  Beta-tricalcium phosphate  on physical, mechanical and biological properties  of poly(3-hydroxybutyrate) / chitosan electrospun scaffold for cartilage tissue engineering applications, Mat. Tech: Adv. Perform. Mat. 34 (2019) 615-625.

[16] R. Iron, M. Mehdikhani, E. Naghszargar, S. Karbasi, D. Semnani, Effects of nano-bioactive glass on structural, mechanical and bioactivity properties of poly (3-hydroxybutyrate) electrospun for bone tissue engineering, Mat. Tech: Adv. Perform. Mat. 34 (2019) 540-548.

[17] Khajeh – Sharafabadi A, et.al. A novel and economical route for synthesizing akermanite (Ca₂MgSi₂O₇) nano – bioceramic. J Mater SCi Eng C. http:// dx.doi.org/10.1016/j. msec. 2016.11.021.

[18] Choudhary R, et.al. In vitro bioactivity, biocompatibility and dissolution studies of diopside prepared from biowaste by using sol-gel combustion method. J Mater Sci Eng C. 2016; 68: 89-100.

[19]A.K. Mohanty, M. Mirsa, G. Hinrichsen, “Biofibers, biodegradable polymers and biocomposites: an overview”, Journal of Macromolecules Materials Engineering, Vol.276, pp.1-24, 2000.

[20] J.M. Anderson, M.S. Shive, “Biodegradation and biocompatibility of PLA and PLGA microspheres”, Journal of. Advanced Drug Delivery Reviews, Vol.64, pp.72-82, 2012.

[21] T.K. Dash, V. Badireenath Konkimalla, “Polycaprolactone based formulations for drug delivery and tissue engineering: A review”, Journal of Controlled Release, Vol.158, pp.15-33, 2012.

[22]L.C. Gerhardt, A.R. Boccaccini, “Bioactive glass and glass-ceramic scaffolds for bone tissue engineering”, Journal of Materials Science, Vol.3, pp.3867-3910, 2010.

[23]A. Najafinezhad, M. Abdellahi, H. Ghayour, A. Soheily, A. Chami, A. Khandan, “A comparative study on the synthesis mechanism, bioactivity and mechanical properties of three silicate bioceramics”, Journal of Materials Science Engineering C, Vol.72, pp.259 – 267, 2017.

[24] A. Sionkowska, J. Kozlowska, “Properties and modification of porous 3 – D collagen / hydroxyapatite composites”, International Journal of Biological Macromolecules, Vol.52, pp.250 – 259, 2013.

[25] T. Kokubo, H.Takadama, “How useful is SBF in predicting in vivo bone bioactivity?”, J. Biomaterials, Vol.27, 2907-2915, 2006.

[26] H. Ghomi, M. Jaberzadeh, M.H. Fathi, “Novel fabrication of forsterite scaffold with improved mechanical properties”, Journal of Alloys and Compounds, Vol.509, pp.63-68, 2011.

[27] M.H. Fathi, M. Kharaziha, “Two-step sintering of dense, nanostructural forsterite”, Journal of Materials Letters, Vol.63, pp.1455-1458, 2009.

[28] Y.C. Lim, J. Johnson, Z. Fei, Y. Wu, D.F. Farson, J.J. Lannutti, H.W. Choi, L. Lee, “Micropatterning and Characterization of electrospun poly (epsilon – caprolactone)/ gelatin nanofiber tissue scaffolds by femtosecond laser ablation for tissue engineering applications”, Journal of Biotechnology Bioengineering, Vol.108, pp.116126, 2011.

[29] L. Ghasemi – Mobarakeh, M.P. Prabhakaran, M. Morshed, M.H. Nasr – Esfahani, S. Ramakrishna, “Bio – functionalized PCL nanofibrous scaffold for nerve tissue engineering”, Journal of Materials Science and Engineering C, Vol.30, pp.1129-1136, 2010.

[30] C. Vitale – Brovarone, M. Miola, C. Balagna, E. Verne, “3D – glass – ceramic scaffolds with antibacterial properties for bone grafting”, Journal of Chemical Engineering, Vol.137, pp.129 – 136, 2008.

[31] C.V. Brovarone, E. Verne, P.F. Appendino, “Macro-porous bioactive glass – ceramic scaffolds for tissue engineering”, Journal of Materials Science: Materials in Medicine, Vol.17, pp.1069 – 1078, 2006.

[32] D.R. Carter, W.C. Mayes, “Bone compressive strength: the influence of density and strain rate”, Journal of Science, Vol.194, pp.1174-1176, 1976. [33] L.L. Hench, J. Wilson, “An introduction to Bioceramics”, world scientific, publishing, Co LTd, London, 1993.