Green Synthesis of Nanoceria (CeO2) and Evaluation of Enzyme like Characteristics
Subject Areas : Bio Materials
Azize
Alayli Gungor
1
(Ataturk University, Erzurum Vocational Training School, Department of Chemical Technology, 25240 Erzurum, Turkey)
Hayrunnisa
Nadaroglu
2
(Ataturk University, Erzurum Vocational Training School, Department of Food Technology, 25240 Erzurum, Turkey)
Aynur
Babagil
3
(Ataturk University, Faculty of Engineering, Department of Nano-Science and Nano-Engineering, 25240 Erzurum, Turkey)
Hicran
Onem
4
(Ataturk University, Faculty of Engineering, Department of Nano-Science and Nano-Engineering, 25240 Erzurum, Turkey)
Keywords: Peroxidase, Ceria nanoparticles, Bio reduction, Fig (Ficus carica),
Abstract :
In this study, the synthesis of ceria (CeO2) nanoparticles (NPs) was examined by the biosynthesis method. Then, enzyme-like features of synthesized nanoceria were examined. Peroxidase enzyme from fig (Ficus carica) was used as a synthesis and stabilizer reagent. Furthermore, it was investigated whether the obtained nanoceria has superoxide dismutase (SOD), catalase (CAT) and peroxidase (POX) activities. UV-VIS absorption spectroscopy was employed for monitoring of creation of ceria nanoparticles. The characteristics of the obtained ceria nanoparticles were studied with X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM). Also, cerium oxide NPs showed enzyme-like activities, and its activities were determined with specific enzyme activity measuring methods. Surface morphology and size of the synthesized ceria were investigated by chromatographic techniques. The diameter of the biosynthesized ceria nanoparticles was determined to be 14 nm using XRD chromatogram. Advantages of unique properties of nanoceria have been promising for being enzyme-like reagent in nano-biotechnological investigations. This research explored and discussed if ceria nanomaterial s different kinds of enzymes. We examined their kinetics, mechanisms and applications, including in superoxide dismutase (SOD), catalase (CAT), and peroxidase (POX) activities. The results showed that the ceria oxide nanoparticles exhibited catalase, peroxidase and superoxide dismutase activities.