Hydroxyapatite Nano-Particles Extracted from Miscellaneous Bestial Resources: Study of Crystal Structure and Microstructure

Subject Areas : Bio Materials

Seyed Mehdi Rafiaei ¹ , Mohammad Khodaei ² , Fateme Eslami ³

1 - Department of Materials Science and Engineering, Golpayegan College of Engineering, Isfahan University of Technology, Golpayegan 87717-67498, Iran
2 - Department of Materials Science and Engineering, Golpayegan College of Engineering, Isfahan University of Technology, Golpayegan 87717-67498, Iran
3 - Department of Materials Science and Engineering, Golpayegan College of Engineering, Isfahan University of Technology, Golpayegan 87717-67498, Iran

Received: 2020-06-03 Accepted : 2020-07-02 Published : 2020-08-01

Keywords: hydroxyapatite, Fish, Hen and Bovine Bones, Microstructural properties,

Abstract :

In this work, hydroxyapatite nano-particles were successfully synthesized via the usage of available fish, hen and bovine bones in waste foods. The crystal structure, microstructure, and functional groups of the synthesized nanostructured bioceramics were studied using X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), and fourier transform infrared spectroscopy (FTIR). It was shown that all the used natural resources provide well crystallized crystal structures of hydroxyapatite with a narrow distribution microstructure in the range of 24.4-93.4 nm. As well, it was found that the hydroxyapatite extracted from the fish bone has smaller particle size than those of the hen and bovine. Additionally, all the synthesized productions possess the same functional groups, they can be employed as promising candidates for the bone filler materials in biomedical engineering.

References:

[1] A. Märten, P. Fratzl, O. Paris, P. Zaslansky, On the mineral in collagen of human crown dentine, Biomaterials 31 (2010) 5479-90.

[2] M. Itokazu, W. Yang, T. Aoki, A. Ohara, N. Kato, Synthesis of antibiotic-loaded interporous hydroxyapatite blocks by vacuum method and in vitro drug release testing, Biomaterials 19 (1998) 817-819.

[3] Q. Lv, L. Nair, C.T. Laurencin, Fabrication, characterization, and in vitro evaluation of poly(lactic acid glycolic acid)/nano-hydroxyapatite composite microsphere-based scaffolds for bone tissue engineering in rotating bioreactors, Journal of Biomedical Materials Research 91 (2009) 679-691.

[4] A. Alyahya, G. R. J. Swennen, Bone grafting in orthognathic surgery: a systematic review, International Journal of Oral and Maxillofacial Surgery 48 (2019) 322-331.

[5] T. Mokabber, Q. Zhou, A. I. Vakis, P. van Rijn, Y. T. Pei, Mechanical and biological properties of electrodeposited calcium phosphate coatings, Materials Science and Engineering: C 100 (2019) 475-484.

[6] M. Sadat-Shojai, M. T. Khorasani, E. Dinpanah-Khoshdargi, A. Jamshidi, Synthesis methods for nanosized hydroxyapatite with diverse structures, Acta Biomaterialia 9 (2013) 7591-7621.

[7] T. M. Coelho, E. S. Nogueira, A. Steimacher, A. N. Medina, W. R. Weinand, W. M. Lima, M. L. Baesso, and A. C. Bento, Characterization of natural nanostructured hydroxyapatite obtained from the bones of Brazilian river fish, Journal of Applied Physics 100 (2006) 094312-1 094312-6.

[8] A. Ruksudjarit, K. Pengpat, G. Rujijanagul, T. Tunkasiri, Synthesis and characterization of nanocrystalline hydroxyapatite from natural bovine bone, Current Appllied Physics 8 (2008) 270-272.

[9] M. Boutinguiza, J. Pou, R. Comesaña, F. Lusquiños, A. de Carlos, B. León, Biological hydroxyapatite obtained from fish bones Materials Science and Engineering: C 32 (2012) 478-486.

[10] G. Gergely, F. Wéber, I. Lukács, A. L. Tóth, Z. E.Horváth, J. Mihály, C. Balázsi, Preparation and characterization of hydroxyapatite from eggshell, Ceramics International 36 (2010) 803-806.

[11] R. Nicula, F. Luthen, M. Stir, B. Nebe, E. Burkel, Spark plasma sintering synthesis of porous nanocrystalline titanium alloys for biomedical applications, Biomolecular Engineering 24 (2007) 564-567.

[12] S. Jinawath, D.Pongkao, M. Yoshimura, Hydrothermal synthesis of hydroxyapatite from natural bone, Journal of Materials Science 13 (2002) 491-494.

[13] M. E. Bahrololooma, M. Javidia, S. Javadpoura, Characterisation of natural hydroxyapatite extracted from bovine cortical bone ash”, Journal of Ceramic Processing Research 10 (2009) 129 -138.

[14]C.Y. Ooi, M. Hamdi, S. Ramesh, Properties of hydroxyapatite produced by annealing of bovine bone, Ceramics International 33 (2007) 1171-1177.

[15] A. Monshi, M. Foroughi, M. Monshi, Modified Scherrer Equation to Estimate More Accurately Nano- Crystallite Size Using XRD, Scientific Research 2 (2012) 154-160.

[16] S.M. Rafiaei, Synthesis of YVO₄:Eu³⁺ nanophosphors through the use of urea and aniline fuels and study of their microstructures and optical properties, Transactions of the Indian Institute of Metals, 70 (2017), No. 6, 1543–1547.

[17] S.M. Rafiaei, A. Bahrami, The effects of boric acid on crystal structure, nano/microstructure and photoluminescence characteristics of rare earth doped Y₂O₃/YBO₃ composite compounds, Journal of Nanostructure in Chemistry, 7 (2017), 367-373.

[18] S.I. Roohani-Esfahani, S. Nouri-Khorasani, Z. Lu, R. Appleyard, H. Zreiqat, The influence hydroxyapatite nanoparticle shape and size on the properties of biphasic calcium phosphate scaffolds coated with hydroxyapatite-PCL composites, Biomaterials. 2010 (21) 5498-509.

[19] L. Lutterotti, Total pattern fitting for the combined, size-strain-stress-texture determination in thin film diffraction, Nuclear Inst. and Methods in Physics Research, B 268 (2010) 334-340.

[20] L. Lutterotti, M. Bortolotti, G. Ischia, I. Lonardelli and H.-R. Wenk, Rietveld texture analysis from diffraction images", Z. Kristallogr., Suppl. 26 (2007) 125-130.

[21] L. Lutterotti, D. Chateigner, S. Ferrari and J. Ricote, Texture, Residual Stress and Structural Analysis of Thin Films using a Combined X-Ray Analysis, Thin Solid Films, 450 (2004) 34-41.

[22] S.V. Dorozhkin, Nanosized and nanocrystalline calcium orthophosphates, Acta Biomaterialia 6 (2010) 715-734.

[23] J. Reyes-Gasga, E.L. Martínez-Piñeiro, G. Rodríguez Álvarez, G.E. Tiznado-Orozco, R. García-García, E.F. Brès, XRD and FTIR crystallinity indices in sound human tooth enamel and synthetic hydroxyapatite, Materials Science and Engineering C 33 (2013) 4568–4574.

[24] M. Du, J. Chen, K. Liu, H. Xing, C. Song, Recent advances in biomedical engineering of nano-hydroxyapatite including dentistry, cancer treatment and bone repair, Composites Part B: Engineering 215 (2021) 108790.

[25] L. A. Hasan, Evaluation the properties of orthodontic adhesive incorporated with nano-hydroxyapatite particles, The Saudi Dental Journal, in press (2021), DOI: https://doi.org/10.1016/j.sdentj.2021.01.001.

[26] M. Fahami, M. Abdellahi, Estimation of the Mean Grain Size of Mechanically Induced Hydroxyapatite Based Bioceramics via Artificial Neural Network, Journal of Advanced Materials and Processing 5(2) (2017) 52-60.

[27] A. Ruksudjarit, K. Pengpat, G. Rujijanagul, T.Tunkasiri, Synthesis and characterization of nanocrystalline hydroxyapatite from natural bovine bone, Current Applied Physics 8 (3-4) (2008) 270-272.