The effect of Malva neglecta total extract on bleomycin- induced pulmonary fibrosis in rat
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
Amir Albabay
1
,
Ali Hosseini Sharifabad
2
,
Afsaneh Yegdaneh
3
,
Mahdi Tavakoli
4
,
Hamide Dabaghydost
5
1 - School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Science, Esfahan, Iran;
2 - School of Pharmacy and Pharmaceutical Sciences
3 - Department of Pharmcognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Science, Esfahan, Iran
4 - Department of Pharmacognosy, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
5 - School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Science, Esfahan, Iran;
Keywords: Idiopathic pulmonary fibrosis, Bleomycin, Malva neglecta, Rat,
Abstract :
Background & Aim: Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive respiratory disease. Malva neglecta possesses significant anti-inflammatory and antioxidant activity. In this study, the therapeutic effect of hydroalcoholic extract of M. neglecta on bleomycin-induced pulmonary fibrosis was investigated. Experimental: 36 male Wistar rats (180-200 g) were used for this study. The animals were randomly divided into six groups. Group I (control) was administered saline intratracheally on day 1 (single dose). The other groups received a single dose of bleomycin (7.5 IU/kg) intratracheally on day 1 and were then fed daily for 28 days according to the following schedule: group II: saline, group III: pirfenidone, groups IV, V, VI: 300, 600 or 900 mg/kg M. neglecta extract. Finally, the rats were euthanized and the lung tissue was removed for histological analyses and biochemical investigations. Results: Intratracheal administration of bleomycin significantly increased the lung tissue levels of hydroxyproline, malondialdehyde and free radicals compared to the control group (P<0.001). M. neglecta extract (900 mg/kg) significantly prevented the increase of these factors compared to the BLM group (P<0.001). M. neglecta extract at higher dose (900 mg/kg) remarkably decreased the above factors compared to its lower dose (P<0.01). Lung tissue in bleomycin-treated groups showed severe tissue damage. In addition, hydroalcoholic extracts from M. neglecta prevented the pathological damage of BLM to the lung tissue. Recommended applications/industries: Our findings showed M. neglecta total extract could treat the pulmonary fibrosis induced by BLM. This effect may be related to the anti-inflammatory and antioxidant properties of the extract.
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