The effect of Malva neglecta total extract on bleomycin- induced pulmonary fibrosis in rat
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
Amir Albabay
1
,
Ali Hosseini Sharifabad
2
*
,
Afsaneh Yegdaneh
3
,
Mahdi Tavakoli
4
,
Hamide Dabaghydost
5
1 - School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Science, Esfahan, Iran;
2 - School of Pharmacy and Pharmaceutical Sciences
3 - Department of Pharmcognosy, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Science, Esfahan, Iran
4 - Department of Pharmacognosy, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran.
5 - School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Science, Esfahan, Iran;
Keywords: Idiopathic pulmonary fibrosis, Bleomycin, Malva neglecta, Rat,
Abstract :
Background & Aim: Idiopathic pulmonary fibrosis (IPF) is a chronic and progressive respiratory disease. Malva neglecta possesses significant anti-inflammatory and antioxidant activity. In this study, the therapeutic effect of hydroalcoholic extract of M. neglecta on bleomycin-induced pulmonary fibrosis was investigated. Experimental: 36 male Wistar rats (180-200 g) were used for this study. The animals were randomly divided into six groups. Group I (control) was administered saline intratracheally on day 1 (single dose). The other groups received a single dose of bleomycin (7.5 IU/kg) intratracheally on day 1 and were then fed daily for 28 days according to the following schedule: group II: saline, group III: pirfenidone, groups IV, V, VI: 300, 600 or 900 mg/kg M. neglecta extract. Finally, the rats were euthanized and the lung tissue was removed for histological analyses and biochemical investigations. Results: Intratracheal administration of bleomycin significantly increased the lung tissue levels of hydroxyproline, malondialdehyde and free radicals compared to the control group (P<0.001). M. neglecta extract (900 mg/kg) significantly prevented the increase of these factors compared to the BLM group (P<0.001). M. neglecta extract at higher dose (900 mg/kg) remarkably decreased the above factors compared to its lower dose (P<0.01). Lung tissue in bleomycin-treated groups showed severe tissue damage. In addition, hydroalcoholic extracts from M. neglecta prevented the pathological damage of BLM to the lung tissue. Recommended applications/industries: Our findings showed M. neglecta total extract could treat the pulmonary fibrosis induced by BLM. This effect may be related to the anti-inflammatory and antioxidant properties of the extract.
Abidi, A., Robbe, A., Kourda, N., Khamsa, S.B. and Legrand, A. 2017. Nigella sativa, a traditional Tunisian herbal medicine, attenuates bleomycin-induced pulmonary fibrosis in a rat model. Biomedicine and Pharmacotherapy, 90: 626-637.
Afolayan, A.J., Aboyade, O.M., Adedapo, A.A. and Sofidiya, M.O. 2010. Anti-inflammatory and analgesic activity of the methanol extract of Malva parviflora Linn (Malvaceae) in rats. African Journal of Biotechnology, 9(8):1225-1229.
Afshari-Kaveh, M., Abbasalipourkabir, R., Nourian, A. and Ziamajidi, N. 2021. The protective effects of vitamins A and E on titanium dioxide nanoparticles (nTiO2)-induced oxidative stress in the spleen tissues of male Wistar rats. Biological Trace Element Research, 199:3677-3687.
Akgedik, R., Akgedik, Ş., Karamanlı, H., Uysal, S., Bozkurt, B., Ozol, D., et al. 2012. Effect of resveratrol on treatment of bleomycin-induced pulmonary fibrosis in rats. Inflammation, 35:1732-1741.
Ashcroft, T., Simpson, J.M. and Timbrell, V. 1988. Simple method of estimating severity of pulmonary fibrosis on a numerical scale. Journal of Clinical Pathology, 41(4):467-470.
Esterbauer, H., Schaur, R.J. and Zollner, H. 1991. Chemistry and biochemistry of 4-hydroxynonenal, malonaldehyde and related aldehydes. Free Radical Biology and Medicine, 11(1):81-128.
Gao, C., Bai, Y., Zhou, H., Meng, H., Wu, T., Bai, W., et al. 2023. Effects of N-butanol extract of Amygdalus mongolica on rats with bleomycin‐induced pulmonary fibrosis based on metabolomics. Brazilian Journal of Medical and Biological Research, 56:e13045.
Ghasemi Dehkordi, N., Sajadi, S., Ghanadi, A., Amanzadeh, Y., Azadbakht, M., Asghari, G.R., et al. 2002. Iranian herbal pharmacopoeia. Tehran: Ministiry of health and medical education. 6(3):63-69.
Glass, D.S., Grossfeld, D., Renna, H.A., Agarwala, P., Spiegler, P., DeLeon, J. and Reiss, A.B. 2022. Idiopathic pulmonary fibrosis: Current and future treatment. The Clinical Respiratory Journal, 16(2): 84-96.
Gopalakrishnan, A., Ram, M., Kumawat, S., Tandan, S. and Kumar, D. 2016. Quercetin accelerated cutaneous wound healing in rats by increasing levels of VEGF and TGF-β1. Indian Journal of Experimental Biology, 54(3):187-195.
Güder, A. and Korkmaz, H. 2012. Evaluation of in-vitro antioxidant properties of hydroalcoholic solution extracts Urtica dioica L., Malva neglecta Wallr. and their mixture. Iranian Journal of Pharmaceutical Research, 11(3):913.
Gungor, H., Ekici, M., Onder Karayigit, M., Turgut, N.H., Kara, H. and Arslanbas, E. 2020. Zingerone ameliorates oxidative stress and inflammation in bleomycin-induced pulmonary fibrosis: modulation of the expression of TGF-β1 and iNOS. Naunyn-Schmiedeberg's Archives of Pharmacology, 393:1659-1670.
Guo, J., Yang, Z., Jia, Q., Bo, C., Shao, H. and Zhang, Z. 2019. Pirfenidone inhibits epithelial-mesenchymal transition and pulmonary fibrosis in the rat silicosis model. Toxicology Ltters, 300:59-66.
Hasan, M., Paul, N.C., Paul, S.K., Saikat, A.S.M., Akter, H., Mandal, M. and Lee, S.S. 2022. Natural product-based potential therapeutic interventions of pulmonary fibrosis. Molecules, 27(5):1481.
Hosseini-Sharifabad, A., Sadraei, H., Hashemnia, M., Sajjadi, S.E. and Mirdamadi, Z. 2021. Effect of hydroalcoholic and aqueous extracts of Dracocephalum kotschyi on bleomycin induced pulmonary fibrosis. Journal of Herbmed Pharmacology, 10(2): 209-217.
Hübner, R-H., Gitter, W., Eddine, E., Mokhtari, N., Mathiak, M., Both, M., Bolte, H., et al. 2008. Standardized quantification of pulmonary fibrosis in histological samples. Biotechniques, 44(4): 507-517.
Kim, H.J., Perlman, D. and Tomic, R. 2015. Natural history of idiopathic pulmonary fibrosis. Respiratory Medicine, 109(6): 661-670.
Kravis, T.C., Ahmed, A., Brown, T., Fulmer, J. and Crystal, R. 1976. Pathogenic mechanisms in pulmonary fibrosis: collagen-induced migration inhibition factor production and cytotoxicity mediated by lymphocytes. The Journal of Clinical Investigation, 58(5): 1223-1232.
Kroes, Bv., Van den Berg, A., Van Ufford, H.Q., Van Dijk, H. and Labadie, R. 1992. Anti-inflammatory activity of gallic acid. Planta medica, 58(06): 499-504.
Marouane, W., Soussi, A., Murat, J-C., Bezzine, S. and El Feki, A. 2011. The protective effect of Malva sylvestris on rat kidney damaged by vanadium. Lipids in Health and Disease, 10:1-8.
Mouratis, M.A. and Aidinis, V. 2011. Modeling pulmonary fibrosis with bleomycin. Current Opinion in Pulmonary Medicine, 17(5):355-361.
Mousavi, M., Abedimanesh, N., Mohammadnejad, K., Sharini, E., Nikkhah, M., Eskandari, M.R., et al. 2022. Betanin alleviates oxidative stress through the Nrf2 signaling pathway in the liver of STZ-induced diabetic rats. Molecular Biology Reports. 49(10):9345-9354.
Nwafor, E-O., Lu, P., Liu, Y., Peng, H., Qin, H. and Zhang, K. 2021. Active components from traditional herbal medicine for the potential therapeutics of idiopathic pulmonary fibrosis: a systemic review. The American Journal of Chinese Medicine, 49(05):1093-1114.
Romero, F.J., Bosch-Morell, F., Romero, M.J., Jareño, E.J., Romero, B., Marín, N. and Romá, J. 1998. Lipid peroxidation products and antioxidants in human disease. Environmental Health Perspectives, 106(suppl 5):1229-1234.
Safaeian, L., Jafarian, A., Rabbani, M., Sadeghi, H., Torabinia, N. and Alavi, S. 2008. The role of strain variation in BAX and BCL-2 expression in murine bleomycin-induced pulmonary fibrosis. Pakistan Journal of Biological Sciences, 11(23):2606.
Sankari, A., Chapman, K. and Ullah, S. 2024. Idiopathic pulmonary fibrosis. StatPearls Publishing. PMID: 28846333.
Saleem, U., Khalid, S., Zaib, S., Anwar, F., Ahmad, B., Ullah, I., et al. 2020. Phytochemical analysis and wound healing studies on ethnomedicinally important plant Malva neglecta Wallr. Journal of Ethnopharmacology, 249:112401.
Samareh Fekri, M., Poursalehi, H.R., Sharififar, F., Mandegary, A., Rostamzadeh, F. and Mahmoodi, R. 2021. The effects of methanolic extract of Glycyrrhiza glabra on the prevention and treatment of bleomycin-induced pulmonary fibrosis in rat: experimental study. Drug and Chemical Toxicology, 44(4):365-371.
Seyyednejad, S.M., Koochak, H., Darabpour, E. and Motamedi, H. 2010. A survey on Hibiscus rosa—sinensis, Alcea rosea L. and Malva neglecta Wallr as antibacterial agents. Asian Pacific Journal of Tropical Medicine, 3(5):351-355.
Soltanfar, A., Meimandi Parizi, A., Foad-Noorbakhsh, M., Sayyari, M. and Iraji, A. 2023. The healing effects of thymoquinone on experimentally induced traumatic tendinopathy in rabbits. Journal of Orthopaedic Surgery and Research, 18(1):233.
Wynn, T.A. and Ramalingam, T.R. 2012. Mechanisms of fibrosis: therapeutic translation for fibrotic disease. Nature Medicine, 18(7):1028-1040.