Analysis of the correlation between phytochemical content and wound-healing potential of Lantana camara ethyl acetate and methanol extracts

Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)

آیرین  آگبو ¹ (گروه شیمی، دانشگاه نلسون ماندلا، پورت الیزابت، آفریقای جنوبی.)
بوییسوا  هلانگوتی ² (گروه شیمی، دانشگاه نلسون ماندلا، پورت الیزابت، آفریقای جنوبی.)
جنسکه  دیدلوف ³ (گروه بیوشیمی و میکروبیولوژی، دانشگاه نلسون ماندلا، پورت الیزابت، آفریقای جنوبی.)
آنا  هاتینگ ⁴ (گروه بیوشیمی و میکروبیولوژی، دانشگاه نلسون ماندلا، پورت الیزابت، آفریقای جنوبی.)
لاون  ونابلس ⁵ (گروه بیوشیمی و میکروبیولوژی، دانشگاه نلسون ماندلا، پورت الیزابت، آفریقای جنوبی.)
شارلین  گووندر ⁶ (گروه بیوشیمی و میکروبیولوژی، دانشگاه نلسون ماندلا، پورت الیزابت، آفریقای جنوبی.)
مارینا  ون دی ونتر ⁷ (گروه بیوشیمی و میکروبیولوژی، دانشگاه نلسون ماندلا، پورت الیزابت، آفریقای جنوبی.)

Received: 2023-03-22 Accepted : 2023-06-29 Published : 2023-06-22

Keywords: Streptococcus pyogenes, Wound, Lantana camara, Micro-broth dilution,

Abstract :

Background & Aim: Lantana camara, regarded as an invasive plant, is found in tropical climates worldwide. Several studies have validated its antiulcer activity which is one of the ethnomedicinal uses of L. camara. This study seeks to find the correlation between the phytochemical content and the wound-healing potential of L. camara extracts.Experimental: The study was conducted using spectrophotometric analysis and selected in vitro bioactivity assays; 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT), inhibitory effect on nitric oxide production in lipopolysaccharide-activated RAW 264.7 macrophages, and 96-well plate micro-broth dilution method assay, of methanol flower (MLF), berry (MLB), leaf (MLL) and ethyl acetate flower (ELF), berry (ELB), leaf (ELL) extracts for cytotoxicity, anti-inflammatory and antimicrobial activities which support wound-healing capabilities.Results: The highest total polyphenolic content (TPC) was recorded in MLF and ELF extracts (34.59±3.01; 29.50±2.11, mg GAEQ/100 mg). MLF and MLB retained the highest terpenoid concentration (20.74±2.34; 20.51±1.86 mg LIN EQ/100 mg). ELB exhibited anti-inflammatory activity at 200 μg/mL. MLF and ELB were nontoxic to Vero cells, while MLL and MLB caused < 20 % cell death at all concentrations investigated. All ethyl acetate extracts exhibited potent antimicrobial activities against Streptococcus pyogenes with both ELL and ELF extracts demonstrating inhibition with MIC ≤ 0.125 mg/mL. The results showed MLF which retained the highest TPC was nontoxic whereas ELL, with relatively high alkaloid content, was the most cytotoxic extract that exhibited growth inhibition to S. pyogenes at a significantly low MIC. Thus, the phytochemical content of extracts is positively associated with bioactivity. The nontoxic, anti-inflammatory, and antimicrobial activities support the wound-healing potentials of the extracts.Recommended applications/industries: The high phenolic content and microbial growth inhibition exhibited could be leveraged to develop wound-healing tinctures or isolate bioactive compounds that could be developed as wound-healing agents.

References:

Agbo, I.A., Hlangothi, B., Didloff, J., Hattingh, A.C., Venables, L., Govender, S. and De Venter, M. 2023. Comparative evaluation of the phytochemical contents, antioxidant and some biological activities of Khaya grandifoliola methanol and ethyl acetate stem bark, root, and leaf extracts. Tropical Journal of Natural Product Research, 7: 2829-2836.

Agbo, M.O., Uzor, P.F., Nneji, U.N.A., Odurukwe, C.U.E., Ogbatue, U.B. and Mbaoji, E.C. 2015. Antioxidant, total phenolic, and flavonoid content of selected Nigerian medicinal plants. Dhaka University Journal of Pharmaceutical Sciences, 14: 35-41.

Akumu, E.O., Swamy, A.T., Mutuku, C.N., Obey, J.K. and Miyogo, E. 2014. Pharmacological evaluation of Lantana camara leaf smoke. International Journal of Pharmacy and Biological Sciences, 4: 28-34.

Altemimi, A., Lakhsassi, N., Baharlouei, A., Watson, D.G. and Lightfoot, D.A. 2017.Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6: 1-23.

Desalu, O.O., Salawu, F.K., Jimoh, A.K., Adekoya, A.O., Busari, O.A. and Olokoba, A.B. 2011. Diabetic foot care: Self-reported knowledge and practice among patients attending three tertiary hospitals in Nigeria. Ghana Medical Journal, 45: 60-65.

do Carmo, M.A.V., Pressete, C.G., Marques, M.J., Granato, D. and Azevedo, L. 2018. Polyphenols as potential antiproliferative agents: Scientific trends. Current Opinion in Food Science, 24: 26-35.

Dubey, D., Padhy, R.N. 2013. Antibacterial activity of Lantana camara L. against multidrug-resistant pathogens from ICU patients of a teaching hospital. Journal of Herbal Medicine, 3: 65-75.

Ghuman, S., Ncube, B., Finnie, J.F., McGaw, L.J., Coopoosamy, R.M. and Van Staden, J. 2016. Antimicrobial activity, phenolic content, and cytotoxicity of medicinal plant extracts used for treating dermatological diseases and wound-healing in KwaZulu-Natal, South Africa. Frontiers in Pharmacology, 7: 1-9.

Golparvar, A.R., Gheisari, M.M., Hadipanah, A. and Khorrami, M. 2018. Antibacterial, antifungal properties and chemical composition of essential oils of Satureja hortensis L. and Satureja khuzestanica Jamzad. Journal of Medicinal Herbs, 8: 243-249.

Heping, C., Kandan, S. 2020. Regulation of cell viability and anti-inflammatory Tristetraprolin family gene expression in mouse macrophages by cotton seed extracts. Scientific Reports (Nature Publisher Group), 10: 1-11.

Junio, H.A., Sy-Cordero, A.A., Ettefagh, K.A., Burns, J.T., Micko, K.T., Graf, T.N., Richter, S.J., Cannon, R.E., Oberlies, N.H. and Cech, N.B. 2011. Synergy-directed fractionation of botanical medicines: A case study with golden seal (Hydrastis canadensis). Journal of Natural Products, 74: 1621-1629.

Kalita, S., Kumar, G., Karthik, L. and Rao, K.V.B. 2012. A Review on medicinal properties of Lantana camara Linn. Research Journal of Pharmacy and Technology, 5: 711-715.

Kamboj, A., Gupta, R., Rana, A. and Kaur, R. 2015. Application and analysis of the Folin Ciocalteu method for the determination of the total phenolic content from extracts of Terminalia bellerica. European Journal of Biomedical and Pharmaceutical Sciences, 2: 201-215.

Khan, M., Mahmood, A. and Alkhathlan, H.Z. 2016. Characterization of leaves and flowers volatile constituents of Lantana camara growing in the Central Region of Saudi Arabia. Arabian Journal of Chemistry, 9: 764-774.

Khan, R.M.A., Anwar, A., Pirzada, Z.A. 2020. Streptococcus pyogenes strains associated with invasive and non-invasive infections present possible links with emm types and superantigens. Iranian Journal of Basic Medical Sciences, 2020: 133-139.

Kochman, J., Jakubczyk, K., Antoniewicz, J., Mruk, H. and Janda, K. 2021. Health benefits and chemical composition of matcha green tea: A review. Molecules, 26: 1-11.

Kurin, E., Atanasov, A.G., Donath, O., Heiss, E.H., Dirsch, V.M. and Nagy, M. 2012. Synergy study of the inhibitory potential of red wine polyphenols on vascular smooth muscle cell proliferation. Planta Medica, 78: 772-778.

Mirrezaei, N., Yazdian-Robati, R., Oroojalian, F., Sahebkar, A. and Hashemi, M. 2020. Recent developments in nano-drug delivery systems loaded by phytochemicals for wound healing. Mini-Reviews in Medicinal Chemistry, 20: 1867-1878

Misha, A. 2015. Allelopathic properties of Lantana camara. International Research Journal of Basic and Clinical Studies, 3: 13-28.

Negut, I., Grumezescu, V., Grumezescu, A.M. 2018. Treatment strategies for infected wounds. Molecules, 23: 1-23.

Njayou, F.N., Aboudi, E.C.E., Tandjang, M.K., Tchana, A.K., Ngadjui, B.T. and Moundipa, P.F. 2013. Hepatoprotective and antioxidant activities of stem bark extract of Khaya grandifoliola (Welw) CDC and Entada africana Guill. et Perr. Journal of Natural Products, 6: 73-80.

Osman, M.A., Mahmoud, G.I. and Shoman, S.S. 2020. Correlation between total phenol content, antioxidant power, and cytotoxicity. Biointerface Research in Applied Chemistry, 11: 10640-10653.

Rampa, K.M., Van De Venter, M., Koekemoer, T.C., Swanepoel, B., Venables, L., Hattingh, A.C., Viljoen, A.M. and Kamatou, G.P., 2022. Exploring four South African Croton species for potential anti-inflammatory properties: In vitro activity and toxicity risk assessment. Journal of Ethnopharmacology, 282: 1-11.

Sathish, R., Vyawahare, B., Natarajan, K. 2011. The antiulcerogenic activity of Lantana camara leaves on gastric and duodenal ulcers in experimental rats. Journal of Ethnopharmacology, 134: 195-197.

Shamsa, F., Monsef, H.R., Ghamooghi, R. and VerdianRizi, M.R. 2007. Spectrophotometric determination of total alkaloids in Peganum harmala L. using bromocresol green. Research Journal of Phytochemistry 1: 79-82.

Shedoeva, A., Leavesley, D., Upton, Z. and Fan, C. 2019. Wound-healing and the use of medicinal plants. Evidence-Based Complementary and Alternative Medicine, 2019:1-35.

Shimizu, M. 2017. Multifunction of dietary polyphenols in the regulation of intestinal inflammation. Journal of Food and Drug Analysis, 25: 93-99.

Silva, G.N., Martins, F.R., Matheus, M.E., Leitão, S.G. and Fernandes, P.D. 2005. Investigation of anti-inflammatory and antinociceptive activities of Lantana trifolia. Journal of Ethnopharmacology, 100: 254-259.

Smith, K., Collier, A., Townsend, E.M., O’Donnell, L.E., Bal, A.M., Butcher, J., Mackay, W.G., Ramage, G. and Williams, C. 2016. One step closer to understanding the role of bacteria in diabetic foot ulcers: Characterising the microbiome of ulcers. BMC Microbiology, 16: 1-12.

Surendra, B.S., Mallikarjunaswamy, C., Pramila, S. and Rekha, N.D. 2021. Bio-mediated synthesis of ZnO nanoparticles using Lantana Camara flower extract: Its characterizations, photocatalytic, electrochemical and anti-inflammatory applications. Environmental Nanotechnology, Monitoring & Management,15: 1-10.

Terpinc, P., Čeh, B., Ulrih, N.P. and Abramovič, H. 2012. Studies of the correlation between antioxidant properties and the total phenolic content of different oil cake extracts. Industrial Crops and Products, 39: 210-217.

Thangapazham, R.L., Sharad, S., Maheshwari, R.K. 2016. Phytochemicals in wound healing. Advances in Wound Care, 5: 230-241.

Tzaneva, V., Mladenova, I., Todorova, G. and Petkov, D. 2016. Antibiotic treatment and resistance in chronic wounds of vascular origin. Clujul Medical, 89: 365-370.

Zulkefli, N., Che Zahari, C.N.M., Sayuti, N.H., Kamarudin, A.A., Saad, N., Hamezah, H.S., Bunawan, H., Baharum, S.N., Mediani, A., Ahmed, Q.U. and Ismail, A.F.H., 2023. Flavonoids as potential wound-healing molecules: Emphasis on pathways perspective. International Journal of Molecular 24:1-29.