Histomorphological study of the effect of Biarum straussiis’ rhizome extract on cutaneous wound healing in a rat model
Subject Areas : Journal of Medicinal Herbs, "J. Med Herb" (Formerly known as Journal of Herbal Drugs or J. Herb Drug)
سحر
اکرادی
1
(گروه بیوشیمی، دانشگاه آزاد اسلامی واحد اراک، اراک، ایران)
طاهره
مومنی اصفهانی
2
(گروه بیوشیمی، دانشگاه آزاد اسلامی واحد اراک، اراک، ایران)
محمد
علیمرادی
3
(گروه بیوشیمی، دانشگاه آزاد اسلامی واحد اراک، اراک، ایران)
معصومه
خان احمدی
4
(گروه تحقیقات بیوشیمی جهاد دانشگاهی، واحد کرمانشاه، کرمانشاه، ایران)
Keywords: Wound healing, Biarum straussii, Phytomedicine, Rhizome extract,
Abstract :
Background & Aim:Phytomedicine or herbal medicine, refers to the use of plants to treat diseases and promote good health. Anti-oxidative and anti-inflammatory characteristics of the medicinal herbs make them logical adjuvant to improve wound healing. The aim of this study was to evaluate the wound healing potential of Biarum straussiis’ (B. straussii) rhizome extract on cutaneous wounds in rats.Experimental: Adult male rats (n=18) were divided into three groups (n=6), as group A, B and C. Then, full-thickness, square shape cutaneous wounds were created on the skin. In group A, as negative control, the wound area was only washed using normal saline solution; in group B as positive control, the wound was treated using phenytoin and the wound treatment using B. straussii rhizome extract was done in group C, as experimental subject. The progressive changes in wounds of each group were evaluated for the contraction degree on days 4,7,10 and 14. The tissue samples of the wound area were removed from each group on day 14, fixed in 10% formalin and finally stained with H&E for histological examination. Data analysis was carried out using one-way ANOVA test, followed by Tukey-Kramer test (p <0.05).Results: The wound contraction was higher in group treated with B. straussii extractthan in control group at 7th, 10th and 14th days (p <0.0001). The histological analysis showed a significant accelerated wound contraction, complete re-epithelialization, and tissue recovery due to the topical application of B. straussii rhizome extract.Recommended applications/ industries: It can be concluded that the rhizome extract of B. straussii is favorable for cutaneous wound healing in rats and would be considered as a medicinal plant, but further studies are required to reach more definitive results.
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