بررسی نیترات مازاد در آب آشامیدنی بر روی آنزیم‌های کبدی و هیستوپاتولوژی موش سوری باردار نژاد NMRI
محورهای موضوعی : زیست شناسی سلولی تکوینی گیاهی و جانوری ، تکوین و تمایز ، زیست شناسی میکروارگانیسم
کلید واژه: بارداری, نیترات, کبد, آب آشامیدنی, AST, ALT,
چکیده مقاله :
هدف از این تحقیق بررسی اثرات نیترات مازاد در آب آشامیدنی بر روی آنزیم‌های کبدی و هیستولوژی کبد موش باردار می‌باشد.تعداد 36 سر موش سوری باردار را در روز صفر بارداری به 3 گروه تقسیم شدند.گروه شاهد هیچ نیتراتی دریافت نکرد. گروه تجربی اول mg/l450 نیترات و گروه دوم تجربی دوم مقدار mg/l900 نیترات در آب آشامیدنی افزوده شد. در روز 17 بارداری موش‌های باردار را با اتر بیهوش و از بطن چپ آن‌ها خونگیری و بافت کبد مورد مطالعه هیستوپاتولوژی قرار گرفت.در این تحقیق از نرم‌افزار spss و آنالیز واریانس یک طرفه ANOVA و image jجهت شمارش سلولی استفاده شد.در این مطالعه از نظر ظاهری کاهش وزن موش‌های باردار و از نظر تست‌های بیوشیمیایی افزایش آنزیم‌های کبدی که شامل افزایش معنا‌دار در آسپارتات آمینوترانسفراز و افزایش در آنزیم آلانین آمینوترانسفراز مشاهده شد. هم چنین تغییرات هیستوپاتولوژی در کبد موش باردار سوری نشان داده شد.به نظر می رسد که نیترات مازاد در آب آشامیدنی سبب افزایش آنزیم کبدی و تخریب سلول‌های کبدی می‌شود.
In this study the effects of excess nitrate in drinking water on liver cells of pregnancy mice were studied . For this purpose in this research the effects of the Sodium Nitrate (doses 50, 450,900 mg ∕ liter in drinking water) during pregnancy of female mice were investigated and Blood from the left ventricle and liver tissue was studied histologically. The One-Wey ANOVA and SPSS software were used to determine the statistical significance of differences between the values for the experimental and control groups and also Image J software were used to cell counter. The results obtained showed a significant decrease in Weight of pregnant mice, increase ALT and AST enzymes, and also histological changes. These observations indicate the toxicity of excess nitrates can make histological changes and tissue damages, and increased liver enzymes.
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