اثر آنتی اکسیدانی عصاره هیدروالکلی سیاتوسه (Rhamnus frangula) بر سمیت ناشی از بنزوپیرن در موش
محورهای موضوعی : پاتوبیولوژی مقایسه ای
پوریا قنبری کهیانی
1
,
مریم کریمی دهکردی
2
,
محسن جعفریان دهکردی
3
1 - دانش‌آموخته دکترای حرفه‌ای دامپزشکی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران.
2 - گروه علوم درمانگاهی، دانشکده دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
3 - دانشگاه آزاد اسلامی شهرکرد
کلید واژه: آنتی اکسیدان ها, استرس اکسیداتیو, بنزوپیرن, سیاتوسه, موش, ویتامین E,
چکیده مقاله :
بنزوپیرن به عنوان یک ترکیب کارسینوژن و اکسیداتیو شناخته شده است. سیاتوسه حاوی فلاوونوئیدها با فعالیت آنتی اکسیدانی قوی است که از سلول ها در برابر استرس اکسیداتیو و مرگ سلولی محافظت می کند. هدف از مطالعه حاضر بررسی اثرات آنتی اکسیدانی سیاتوسه در برابر عوارض حاصل از مواجهه با بنزوپیرن در مقایسه با ویتامین E در موش بود. موش های سوری نر به طور تصادفی به 11 گروه کنترل، دریافت کننده روغن زیتون (حلال بنزوپیرن)، ویتامین E، بنزوپیرن، سیاتوسه در سه دوز، بنزوپیرن همراه دوزهای مختلف سیاتوسه و بنزوپیرن به همراه ویتامین E تقسیم شدند. به منظور مقایسه میانگین داده ها از آزمونهای آماری ANOVA و Tukey استفاده شد. بنزوپیرن باعث کاهش آنتی اکسیدانها و افزایش MDA در خون می شود. این تغییرات به طور قابل توجهی در گروه مواجهه یافته با دوزهای بالای سیاتوسه بهبود یافت، درحالیکه تیمار ویتامین E قادر به کاهش استرس اکسیداتیو ناشی از بنزوپیرن نشد. نتایج نشان داد که عصاره هیدروالکلی سیاتوسه با بهبود پارامترهای آنتی اکسیدانی و کاهش پراکسیداسیون لیپیدی، نقش مهمی در برابر سمیت اکسیداتیو بنزوپیرن ایفا می کند که حتی می تواند در حیوانات آزمایشگاهی بهتر از ویتامین E عمل کند.
Benzopyrene is known as a carcinogen compound and an increaser of oxidative stress and apoptosis. Rhamnus frangula L. (Siatooseh) contains flavonoids with strong antioxidant activity that protect cells against oxidative stress and cell death. The aim of the present study was to investigate the antioxidant effects of Siatooseh against the effects of exposure to benzopyrene (BaP) compared to vitamin E in mice. Male mice were randomly divided into 11 normal control groups, receiving olive oil (benzopyrene solvent), vitamin E, BaP, Siatooseh in three doses, BaP with different doses of Siatooseh, and BaP with vitamin E. In order to compare the average data, statistical tests of one-way analysis of variance (ANOVA) and Tukey's test were used. The results showed that BaP injection significantly decreases antioxidants and increases MDA in blood. These changes were significantly improved in the group exposed to high doses of Siatooseh, while vitamin E treatment was not able to reduce the oxidative stress caused by benzopyrene. The results of this study showed that hydroalcoholic extract of Siatooseh plays an important role against oxidative toxicity caused by benzopyrene by improving antioxidant parameters and reducing lipid peroxidation, which can even work better than vitamin E in laboratory animals.
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