Biomonitoring of Heavy Metals in Blood and Urine of African Children from OwerriMetropolis, Eastern Nigeria
Subject Areas : Journal of Chemical Health RisksAndrew Verla Wirnkor 1 , Verla Ngozi 2 , Ajero Medo 3 , Lele Chioma 4 , Ugochukwu Ngozi 5 , Enyoh Christian 6
1 - Group Research in Analytical Chemistry, Environment and Climate Change (GRACE&CC), Department of Chemistry, Imo State University (IMSU), PMB 2000 Owerri, Imo State, Nigeria
2 - Department of Environmental Technology, School of Environmental Technology Federal University of Technology, Owerri, Imo State Nigeria
3 - Department of Animal and Environmental Biology, Imo State University (IMSU), PMB 2000 Owerri, Imo State, Nigeria
4 - Department of Biochemistry, IMSU, Owerri, Imo State, Nigeria
5 - Department of Nursing Science, IMSU Nursing and Health Science, Orlu, Imo State, Nigeria
6 - Group Research in Analytical Chemistry, Environment and Climate Change (GRACE&CC), Department of Chemistry, Imo State University (IMSU), PMB 2000 Owerri, Imo State, Nigeria
Keywords: environment, Toxicity, Trend, concentration, biomarker, Carcinogenic, Diagnostic ratios,
Abstract :
Childhood illnesses have been linked to elevated heavy metals in children’s bodies. Such studies are lacking in developing countries despite the fact that African children could be most exposed to heavy metals. This study determines the concentrations of heavy metals in blood and urine of children in selected schools within Owerri metropolis. Consent and due process were followed to obtain blood and urine samples from 60 children. Samples were digested with nitric and hydrochloric acids for 6 hours at 90o C and analyzed using Perkin Elmer 400 A analyst atomic absorption spectrometer. Mean concentrations in blood was Pb (4.517±1.599 mgl-1); Cd (1.04±0.671 mgl-1); Ni (5.612±1.237 mgl-1); Mn (7.198±4.705 mgl-1) and Cr (0.288 ±0.412 mgl-1). In urine; Pb (1.912±1.219 mgl-1); Cd (0.39±0.138 mgl-1); Ni (3.988±1.230 mgl-1); Mn (3.099±0.990 mgl-1) and Cr (20.773 ±10.449 mgl-1) were generally lower than concentrations in blood. Maximum metals concentrations in blood were higher than values for USA Academy of pediatrics. Except at WBP, Cr was highest concentrations within each school. Order of prevalence (%) was Pb> Ni > Cr >Mn> Cd in blood and Cd >Mn>Pb> Ni > Cr in urine. Variability revealed moderate to high with Cr (143%) as highest for blood while Pb (63.76%) was highest in urine. There was low relationship between metal in blood and urine as R2 values ranged between manganese (0.006) to nickel (0.216). The prominent trend of metal was Mn - Cr as highest and lowest concentrations respectively. Metal diagnostic ratios revealed very undesirable values for Cd (4.05) and Mn (3.545) in blood. Currently no standards for metals in blood and urine for African children, metal concentrations in the present study were elevated. Government agencies and policy makers need to act in time to reduce the potential danger in the near future.
1. Verla A.W.,Verla E.N., 2017. Risk associated with heavy metal concentrations in children playground soils of Owerri metropolis, Imo state, Nigeria. Wor News Nat Sci. WNONS (10), 49-69.
2. Sarah E.C., Ezekiel M., Ericka G.J., Emily C.M., Andrew S.S., Doreen B., Troy C.L.,Ezekiel M.,2018. Assessment of blood levels of heavy metals including lead and manganese in healthy children living in the Katanga settlement of Kampala, Uganda.BMC Pub Health. 18:717 https://doi.org/10.1186/s12889-018-5589-0.
3. Mbah C.N.,AnikweM.A.N., 2010. Variation in heavy metal contents on roadside soils along a major express way in South east Nigeria. New York Sci J. 3(10), 103-107.
4. De Miguel E.I., Iribarren E., Chacon A.O., CharlesworthS., 2006. Risk based evaluation of the exposure of children to trace element in playground in Madrid, Spain.Chemosphere.23(4), 874 – 882.
5. Clozel B., Ruban V., Durand C.,Conil P., 2006. Origin and mobility of heavy metals in contaminated sediments from retention and infiltration ponds.Applied Geochem. (21), 1781–1798.
6. ATSDR (Agency for Toxic Substances and Disease Registry), 2003. Toxicological Profile for Selenium.U.S. Department of Health and Human Services Atlanta, GA, Atlanta, GA, USA.
7. OHCHR., 2016).The Rights of the Child and Hazardous Substances and Wastes.https://www.ohchr.org/EN/Issues/Environment/ToxicWastes/Pages/RightsoftheChildHazardousSubstancesWastes.aspx (Accessed January 04,2019).
8. Goullé J.P., Le Roux P., Castanet M., Mahieu L., Guyet-Job S., Guerbet M., 2015. Metallic profile of whole blood and plasma in a series of 99 healthy children.J Anal Toxicol. 39,707–13
9. Yu-Mei H., Chih-Ying L., Ssu-Ning C., Wei-Jen C., Horng-Sheng S., Shicieu-Rung H., Ming-I L., Shu-Chi M., Ru-Lan H., 2017. Association of blood heavy metals with developmental delay and health status of children. NatSciRepor. 7(43), 6-8.
10. Bocca B.F,2005. Development of methods for the quantification of essential and toxic elements in human biomonitoring.Ann 1st Super Sanita. 41,165-170.
11. Abdel R.G.M., Al-Batanony M.A., Mahrous O.A., Abo- Salem M.E., Gabr H.M., 2012.Environmental lead exposure among primary school children in Shebin El-Kom District, Menoufiya Governorate, Egypt.Int J Occup Environ Med. 3(4), 186–94.
12. Ciidikova P., Sadeva G., Detiradeva R.,Prakova G., Platikonova M., 2015.Blood concentrations of heavy metals amongst environmentally exposed residents of Starta Zagora municipality (Bulgaria). Trakia J Sci. 4, 33-40.
13. Etzel R.A., Pediatric Environmental Health, 2nded Elk Grove Village, IL. American Academy of Pediatrics; 2003; p. 293.
14. Ljung K., Schinus O., Otabbong E., 2006.Metal in soil of children’s urban environment in the small northern European city of Uppsala.SciTotal Environ. 366, 749-759.
15. Onweremadu E.U., Amaechi J.U., Ndukwu B.N.,2011. Vertical distribution of cadmium and lead on soils affected by metropolitan refuse disposal in Owerri, Southeastern Nigeria.Iranica J Ener Environ. 2, 62-67.
16. Akan J.C., Sodipo O.A., Liman Y., Chellube Z.M., 2014. Determination of heavy metals in blood, urine and water samples by inductively coupled plasma atomic emission spectrophotometer and fluoride using ion-selective electrode. J Anal Bioanal Tech. 5(9), 1-7.
17. Verla E.N., Spiff A.I.,Horsfall M.J.,2015.A preliminary survey of heavy metals concentrations in children playground withinOwerri Metropolis, Imo State, Nigeria. Res J Chem Sci. 5(11), 1-6.
18. Jawhar L., Alfred B., Xavier D., Bouchra B., Badiaa L., 2014. Blood lead, cadmium and mercury among children from urban, industrial and rural areas of Fez Boulemane Region (Morocco): relevant factors and early renal effects. Int JOccupMedEnvironHealth. 27(4), 641 – 659.
19. Ogunbileje J.O., SadagoparamanujamV.M., Anetor J.I., Farombi E.O., Akinosun O.M., Okorodudu A.O., 2013. Lead, mercury, cadmium, chromium, nickel, copper, zinc, calcium, iron, manganese and chromium (VI) levels in Nigeria and United States of America cement dust. Chemosphere.90 (11), 2743–9.
20. Orish E.O., Jason L.B., Sabina S., Judith T.Z., 2014. Metal pollution in Nigeria: a biomonitoring update.J Health Pollu. 6, 40-52.
21. Chinedu E.I., Roselyn F. N., Tochi E.E., Uzochukwu O. E., Lucy C. I., Darlinton A. Verla E.N., 2016. Anthropogenic impact and geo-accumulation of heavy metal levels of soils in Owerri, Nigeria, Brit. J ApplSciTechn. 12(1),1-9.
22. Peter H., Helmut D. K., 2006. Biomonitoring of 30 trace metals elements in urine of children and adults by ICP-MS. ClinicaChimica Acta.365, 310-318.
23. Adams S.V., Newcomb P.A., 2014.Cadmium blood and urine concentrations as Measures of exposure: NHANES 1999–2010. J Expo Sci Environ Epidemiol. 24, 163–170.
24. Ha M., Kwan H.J., Leen J.H., Kim H.C., Lee K.J., Park I., Lim Y.W., Lee J.H., Kim Y., SeoJ.H., 2014.Korean Environmental health survey in children and adolescents (KO,EHS-C). Survey designe and pilot study results on selected biomarkers. Int J Hyg Environ Health, 217, 260-270.
25. Cornelis R., Heinzow B., Herber R.F., 1996. Sample collection guidelines for trace elements in blood and urine.IUPAC Commission of Toxicology.J Trace Elem Med Boil. 10, 103-127.
26. Aitio A., Jarvisalo J., 1985. Boilogicalmonitoring of occupational exposure to toxic chemicals.collection processes, storage of specimens. AnnClin Lab Sci. 15, 121-139.
27. Debora E.K., Jennifer B.R., Gwendolyn A.M., 2011. Testing for toxic element: afocus on arsenic, cadmiun, lead and mercury.Lab Medecine. 42(12), 735-742.
28. Okonkwo J.O., Lwenje S.M., Mtetwa V.S., Gumedze P.N., Shilongonyane M.M., 2001.Determination of urinary lead in school children in Manzini, Swaziland, Southern Africa.The Enviromentalist. 21, 205-209.
29.Mostafa G.A., El-Shahawi H.H., Mokhtar A., 2009. Blood lead levels in Egyptian children from high and low lead-polluted areas: Impact on cognitive function. Acta Neurol Scand. 120(1), 30–7.
30. Mahugija J.A.M., Kasenya Z.S., Kilulya K.F., 2018. Levels of heavy metals in urine samples of school children from selected industrial and non-industrial areas in Dar es Salaam, Tanzania. AfriHealth Sci.18(4), 1226-1235.
31. Ahamed M., Verma S., Kumar A., Siddiqui M.K., 2010. Blood lead levels in children of Lucknow, India. EnvironToxicol. 25, 48–54.
32. Cousillas A.Z., Manay N., Pereira L., Alvarez C., Coppes Z., 2005.Evaluation of lead exposure in Uruguayan children. Bull. Environ. Contam.Toxicol.75, 629–36.
33. Health Canada: Report on Human Exposure to Environmental Chemicals in Canada 2010: Results of the biomonitoring component of the 2007–2009 Canadian Health Measures Survey, 2010. http://www.hc-sc.gc.ca/ewhsemt/pubs/contaminants/chms-ecms/section8 (Accessed January 21, 2019).
34. De Burbure C., Buchet J.P., Bernard A., Leroyer A., Nisse C., Haguenoer J.M., 2003.Biomarkers of renal effects in children and adults with low environmental exposure to heavy metals. J Toxicol Environ Health A. 66(9),783–98.
35. Leroyer A., Nisse C., Hemon D., Gruchociak A., Salomez J.L., Haguenoer J.M., 2000. Environmental lead exposure in a population of children in northern France: Factors affecting lead burden. Am J Ind Med. 38, 281–9.
36. Wyrwala E.M., Dziubaneka G., Skrzypek M., Hajok I. 2010. Study of the health effects of long-term exposure to cadmium and lead in a region of Poland, Int. J Environ. Health Res.20(2) 81-86.
37. Bernard A., 2004. Renal dysfunction induced by cadmium: biomarkers of critical effects. Biometals.17, 519-523.
38. Gullegher C.M., Kovach J.S.,Meliker J. R., 2008. Urinary cadmium and osteoporosis in U.S women greater than or equal to 50 years of age: NHANES 1988-1994 and 1999-2004. Environ Health Perspect. 116, 1338-1343.
39. Nawror T., Plusquin M., Hogervorst J., 2006. Environmental exposure to cadmium and risk of cancer: A prospective population- based study. Lancet Oncol. 7, 119-126.
40. Mayumi T., Eiji S., Seieechi M., Rie T., Ayako S., Shunsuke A., Nasafumi S., Chihaya K., Megumi Y., Yasuhiro I., Koichi K., Toshihiro K., 2018.The association between whole blood concentration of heavy metals in pregnant women and premature births: The Japan Environment and children’s study (JES). Environ Res. 166, 562-569.
41. ATSDR, 2013. Environmental Health and Medicine Education.Chromium Toxicity.Clinical Assessment - Laboratory Tests.https://www.atsdr.cdc.gov/csem/csem.asp?csem=10&po=12 (Accessed January 5, 2019).
42. Goullé J., Mahieu L., Castermant J., Neveu N., Bonneau L., Lainé G., Bouige D., Lacroix C., 2005. Metal and Metalloid multi-elementary ICPMS validation in whole blood, plasma, urine and hair Reference values.ForenSci Int.153, 39–44.
43. Domínguez-Cortinas G., Díaz-Barriga F., Martínez-Salinas R.I., Cossío P., Pérez-Maldonado I.N., 2013. Exposure to chemical mixtures in Mexican children: high-risk scenarios. EnvironSciPollu Res Inte.20(1), 351–357
44. Annao W.L., Nurdans D.A.K., Cheung C.C., Mingh W., 2008. Heavy metals concentrations of surface dust from e-waste recycling and its human health implications in Southeast China. Environ SciTechn. 42(7),2671- 2680.
45. Alimonti A., Bocca B., Mattei D., Pino A., 2011. Programme for biomonitoring the Italian Population Exposure (PROBE): internal dose of metals. IstitutoSuperior disanità.
46. Adams J.B., Audhya T., McDonough-Means S., Rubin R.A., Quig D., Geis E., et al., 2012.Toxicological status of children with Autism vs. Nuerotypical children and the Association with Autism Severity.BiolTraElemRes. 151(2), 171-180.
47. WHO., 2000. Air Quality Guidelines For Europe, 2nd Ed, Copenhagen,WHO Regional Publications, European Series No 91.2000.
48. Karri V., Schuhmacher M., Kumar V., 2016. Heavy metals (Pb, Cd, as and MeHg) as risk factors for cognitive dysfunction: a general review of metal mixture mechanism in brain. Environ ToxicolPharmacol. 48, 203–13.
49. Huo X., Peng L., Xu X.J., Zheng LK, Qiu B, Qi ZL, Zhang B, Han D, Piao ZX (2007) Elevated blood lead levels of children in Guiyu, an electronic waste recycling town in China. EnvironHealth Perspect. 15, 1113-1117.
50. Enyoh C.E., Verla A.W., Egejuru N.J., 2018. pHvariations and chemometric assessment of borehole water in Orji, Owerri Imo State, Nigeria. JEnviron Anal Chem. 5(2), 1-9.
51. Adams J., Howsmon D.P., Kruger U., Gies E., Fimbres V., 2017.Significant association of urinary toxic metals and autism-related symptoms – a nonlinear statistical analysis with cross validation.PloS ONE. 12(1), 23-31.
52. Verla E.N.,Horsfall M. J., Orubite K. O., 2016. Total extractible of manganese, cobalt, nickel.copper and zinc in soils of children’s playgrounds within Owerri Metropolis Imo State, Nigeria. Res J ChemSci. 6(3),38-47.
53. De Miguel E., Iribarren I., Chacon E., Ordonez A., Charlesworth S. 2006. Risk based evaluation of the exposure of children to trace element in playground in Madrid (Spain).Chemosphere. 23, 874-882.
54. Ballatori N., 2002. Transport of toxic metals by molecular mimicry.Environ Health Pros.110 (suppl 5), 689-694.