Alpha Lipoic Acid Impact on Apoptosis-Related Gene Expression in Mature Mouse Oocyte
Subject Areas :
Journal of Chemical Health Risks
Zahrah Khezli
1
,
Saeed Zavareh
2
,
Mojdeh Salehnia
3
1 - Anatomy Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2 - School of Biology, Damghan University, Damghan, Iran
3 - Anatomy Department, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Received: 2022-08-22
Accepted : 2023-11-08
Published : 2024-03-01
Keywords:
GV oocytes,
Alpha-lipoic acid,
Apoptotic genes,
Abstract :
Utilizing antioxidants offers a promising strategy for mitigating the effects of oxidative stress. This study was designed to assess the influence of alpha-lipoic acid (ALA) on the maturation of mouse oocytes and their apoptosis-related genes. Germinal vesicle oocytes, obtained from female mice aged 6-8 weeks, were subjected to in vitro maturation. Among these, 488 oocytes were exposed to 100 μM ALA, while 506 oocytes matured without ALA over a 16-hour duration. Subsequent evaluations were conducted to determine oocyte maturation rates. A portion of the mature oocytes at the metaphase II (MII) stage underwent in vitro fertilization, while the remaining oocytes were utilized to analyze the expression of Caspase 3, Bad, Bax, and Bcl2 through real-time RT-PCR. To quantify cell numbers, resulting blastocysts were stained with DAPI (4',6-diamidino-2-phenylindole). The group treated with ALA exhibited significantly higher rates of MII oocytes (77.30%) and a greater proportion of embryos developing into the blastocyst stage (33.87%) in comparison with the control group (55.81% and 25.06%, respectively; P<0.05). Moreover, the average cell count within blastocysts significantly increased in the ALA-treated group (82.37) compared to the control group (68.5; P<0.05). Furthermore, the pro-apoptotic genes expression decreased significantly, while the expression of the Bcl2 gene exhibited a significant increase in the ALA-treated group in comparison to the control group (P<0.05). In conclusion, the supplementation of the maturation medium for mouse oocytes with ALA resulted in improvements in oocyte development and overall embryo quality. This effect was attributed to the downregulation of pro-apoptotic genes and the upregulation of the anti-apoptotic gene.
References:
Yang H., Kolben T., Meister S., Paul C., van Dorp J., Eren S., Kuhn C., Rahmeh M., Mahner S., Jeschke U., 2021. Factors influencing the in vitro maturation (IVM) of human oocyte. Biomedicines. 9(12), 1904.
Telfer E.E., Andersen C.Y., 2021. In vitro growth and maturation of primordial follicles and immature oocytes. Fertility and Sterility. 115(5), 1116-1125.
Scaravelli G., Zacà C., Setti P. E. L., Livi C., Ubaldi F. M., Villani M.T., Greco E., Coccia M.E., Revelli A., Ricci G., 2021. Fertilization rate as a novel indicator for cumulative live birth rate: a multicenter retrospective cohort study of 9,394 complete in vitro fertilization cycles. Fertility and Sterility. 116(3), 766-773.
Gong X., Li H., Zhao Y., 2022. The improvement and clinical application of human oocyte in vitro maturation (IVM). Reproductive Sciences. 29(8), 2127-2135.
Walls M., Hunter T., Ryan J., Keelan J., Nathan E., Hart R., 2015. In vitro maturation as an alternative to standard in vitro fertilization for patients diagnosed with polycystic ovaries: a comparative analysis of fresh, frozen and cumulative cycle outcomes. Hum Reprod. 30(1), 88-96.
Kirillova A., Smitz J.E., Sukhikh G.T., Mazunin I., 2021. The role of mitochondria in oocyte maturation. Cells. 10(9), 2484.
Gao W., Jin Y., Hao J., Huang S., Wang D., Quan F., Zhang M., Zhang J., Ren W., Yu X., 2021. Hydroxyurea affects in vitro porcine oocyte maturation through increased apoptosis and oxidative stress. Bioscience Reports. 41(4), BSR20203091.
Amoushahi M., Salehnia M., Ghorbanmehr N., 2018. The mitochondrial DNA copy number, cytochrome c oxidase activity and reactive oxygen species level in metaphase II oocytes obtained from in vitro culture of cryopreserved ovarian tissue in comparison with in vivo‐obtained oocyte. Journal of Obstetrics and Gynaecology Research. 44(10), 1937-1946.
Malekzadeh Kebria M., Salehnia M., Zavareh S., Moazzeni S.S., 2020. The effect of sodium selenite on apoptotic gene expression and development of in vitro cultured mouse oocytes in comparison with in vivo obtained oocytes. Veterinary Research Forum. 11(4), 377.
Moshaashaee T., Zavareh S., Pourbeiranvand S., Salehnia M., 2021. The Effect of Sodium Selenite on Expression of Mitochondrial Transcription Factor A during In Vitro Maturation of Mouse Oocyte. Avicenna Journal of Medical Biotechnology. 13(2), 81.
Zavareh S., Karimi I., Salehnia M., Rahnama A., 2016. Effect of in vitro maturation technique and alpha lipoic acid supplementation on oocyte maturation rate: focus on oxidative status of oocytes. International Journal of Fertility & Sterility. 9(4), 442.
Zavareh S., Talebi A., Hasanzadeh H., 2015. Amending in vitro culture condition to overcome oxidative stress in assisted reproduction techniques (ART). Archives of Advances in Biosciences. 6(2), 135-148.
Truong T., Gardner D., 2017. Antioxidants improve IVF outcome and subsequent embryo development in the mouse. Human Reproduction. 32(12), 2404-2413.
Hatami S., Zavareh S., Salehnia M., Karimi T. L., Isaac, 2014. Comparison of oxidative status of mouse pre-antral follicles derived from vitrified whole ovarian tissue and vitrified pre-antral follicles in the presence of alpha lipoic acid. J Obstet Gynaecol Res. 40(6), 1680-1688.
Moghimi Khorasgani A., Moradi R., Jafarpour F., Ghazvinizadehgan F., Ostadhosseini S., Heydarnezhad A., Fouladi-Nashta A.A., Nasr-Esfahani M.H., 2021. Alpha-lipoic acid can overcome the reduced developmental competency induced by alcohol toxicity during ovine oocyte maturation. Cell J. 23(2), 164-173.
He Y., Wang Y., Zhang H., Zhang Y., Quan F., 2021. Alpha‐lipoic acid improves the maturation and the developmental potential of goat oocytes in vitro. Reproduction in Domestic Animals. 56(4), 545-554.
He W.S., Sun Y., Li Z., Yang H., Li J., Wang Q., Tan C., Zou B., 2022. Enhanced antioxidant capacity of lipoic acid in different food systems through lipase‐mediated esterification with phytosterols. Journal of the Science of Food and Agriculture. 102(15), 7115.
Rahnama A., Zavareh S., Ghorbanian M.T., Karimi I., 2013. The Effects of cAMP-elevating agents and alpha lipoic acid on in vitro maturation of mouse germinal vesicle oocytes. Journal of Reproduction & Infertility. 14(4), 173.
Hatami S., Zavareh S., Salehnia M., Lashkarbolouki T., Ghorbanian M. T., Karimi I., 2013. Total Oxidative status of Mouse Vitrified Pre-Antral Follicles with Pre-Treatment of Alpha Lipoic Acid. Iranian Biomedical Journal. 18(3), 181-183.
Ghaemi S.R., Salehnia M., Valojerdi M.R., 2008. The effect of progesterone and exogenous gonadotropin on preimplantation mouse embryo development and implantation. Experimental Animals. 57(1), 27-34.
Hatami S., Zavareh S., Salehnia M., Lashkarbolouki T., Ghorbanian M. T., Karimi I., 2014. The impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries. Iranian Journal of Reproductive Medicine. 12(1), 57.
Talebi A., Zavareh S., Kashani M. H., Lashgarbluki T., Karimi I., 2012. The effect of alpha lipoic acid on the developmental competence of mouse isolated preantral follicles. Journal of Assisted Reproduction and Genetics. 29(2), 175-183.
Kafshgiri S.K., Attaranzadeh A., Ghasemi F., 2021. The effect of Alpha-lipoic acid in glyphosate treated granulosa cells on the human folliculogenesis genes. Research Square. 1 (1), 1-16.
Lee A.Y., Lee M.H., Lee S., Cho E.J., 2018. Neuroprotective effect of alpha-linolenic acid against Aβ-mediated inflammatory responses in C6 glial cell. Journal of Agricultural and Food Chemistry. 66(19), 4853-4861.
Bernardi P., Petronilli V., Di Lisa F., Forte M., 2001. A mitochondrial perspective on cell death. Trends in Biochemical Sciences. 26(2), 112-117.
Elmore S., 2007. Apoptosis: a review of programmed cell death. Toxicologic Pathology. 35(4), 495-516.
Zoheir K.M., Harisa G.I., Allam A.A., Yang L., Li X., Liang A., Abd-Rabou A.A., Harrath A.H., 2017. Effect of Alpha lipoic acid on in vitro development of bovine secondary preantral follicles. Theriogenology. 88, 124-130.
Chen S., Liu G., Long M., Zou H., Cui H., 2018. Alpha lipoic acid attenuates cadmium-induced nephrotoxicity via the mitochondrial apoptotic pathways in rat. J Inorg Biochem. 184, 19-26.
