Investigating the Propagation of Seedless, Seeded and Ornamental Barberry Seedlings through Tissue Culture
Subject Areas : Journal of Chemical Health Risks
1 - Department of Agriculture, Damghan Branch, Islamic Azad University, Damghan, Iran
Keywords: Barberry, Tissue Culture, Cultivation environment, Ornamental,
Abstract :
In order to investigate the reproduction of seedless, seeded and ornamental barberry shrubs through tissue culture, a factorial experiment in the form of a completely randomized design in three replications and with three factors, the first factor of three types of barberry shrubs (seedless, seeded and ornamental), the second factor of BAP hormone at four levels (0, 1, 2, 3 mg l-1) and the third factor of IBA hormone at two levels (0 and 0.25 mg l-1) was done in the laboratory of Islamic Azad University, Damghan branch in 2019. The culture medium used was based on Murashige and Stooge (MS). After preparing the culture medium and sterilizing it by autoclave, it was distributed in the test tubes. After preparation, the samples were cultured in a sterile environment and then the samples were stimulated to grow using growth hormones. The investigated traits included germination, number of leaves, length, width and surface of leaves, ratio of width to length of leaves, fresh weight and dry weight of leaves. After collecting information, the data obtained from the experiment was organized and then analysis of variance was performed with SAS statistical software and comparison of means with Duncan's multi-range test. Pearson's correlation coefficient was used in order to determine the relationships of traits. The main effects and some interaction effects on the time of greening, number of leaves, length, width, leaf area and leaf width to length ratio, leaf fresh weight and dry weight were significant. The correlation coefficient shown between the dry weight of leaves with the number of leaves (r=0.88**), leaf length (r=0.74**), leaf width (r=0.74**), leaf area (r=0.75**), green leaf weight(r=0.96**) positive correlation was significant at 1% probability level.
1. Kafi M., Balandari A., 2012. Berberis production and processing technology. Center of Excellence agronomy faculity of agriculture of Ferdowsi University of Mashhad.
2. Balandari A., 1995. The effect of gibberellic acid and ether on fruit characteristics and ease of harvesting seedless barberry. Scientific and Industrial Research Organization of Iran - Khorasan Center.
3. Laei Gh., R. Laei R., Afshari H., 2008. Planting seedless barberry to deal with drought and water shortage crisis, the first national conference of saffron and barberry, Islamic Azad University of Kainat.
4. Sazmand M., Safarnejad Rafiee F., Alamdari. B., 2012a. the effect of growth regulating hormones on direct regeneration in barberry Berberis vulgaris var. Asperma in in vitro conditions, the third national conference of agricultural biotechnology of Iran (vegetable, animal and industrial), Mashhad, Ferdowsi University of Mashhad.
5. Hajizadeh Khanamani R., 2016. The effect of different factors on callus induction in Berberis vulgaris L., Master's thesis in the field of plant breeding. Faculty of Agriculture, Birjand University.
6. Manii A., 1990. Basics of growing fruit trees. Technical Publications of Iran.
7. Shah S.C., singh-Bhujwon H., 1999. Rooting behaviour of berheris cuttings as infjuenced by plant growth regulators.progressive-Horeicujture. 31(1-2), 98-100.
8. Knox G.W., Hamilton D.F., 1982. Rooting of Berberis and ligustrum cultings from stock plants grown at selected light intensities.scienta-horiculturae.16 (1), 85-90.
9. Molinar F.J.R., Mackay W.A., Wall M.M., Carenas M., 1996. Micopropagation of agrita (berberis) Lrifoliat Moric. Hort Sci.31 (6), 1030-1032.
10. Karhu S., Hakala K., 1990. Rooting in vitro of micro propagated barberry (Berberis thunbergii) shoots. Ann. Agr. Fenn. 29, 179-185.
11. Lafferrie J.E., Ahrendt L .W.A., 1977.transfer species and intraspecific texa from mania to berberis Botanicheskii-Zharnal. 82(9), 95-99.
12. Salami M., 2014. Propagation of barberry (Berberis vulgaris L.) by tissue culture method. Master's thesis, Ferdowsi University of Mashhad - Faculty of Agriculture and Natural Resources.
13. Sazmand M., Safarnejad Rafiee F., Alamdari B., 2012. investigation of the effect of different concentrations of plant growth hormones on callus formation of Berberis vulgaris, National Conference on Environment and Plant Production, Semnan, Islamic Azad University, Semnan branch.
14. Ziaratnia M., Salmani A., 2012, Effect of growth regulators on callus induction seedless Berberis (Berberis vulgaris var asperam L.), Third National Conference on Agricultural Biotechnology in Mashhad.
15. Mackay W.A., Molinar Jr. F., Wall M.M., Cardenas M., 1996. Micro propagation of agarita, Berberis trifoliata Moric. Hort Science. 31, 1030-1032.
16. Arena M. E., Pastur G. M., Vater G., 2000. In vitro propagation of Berberis buxifolia Lam. Biocell. 24:73-80.
17. Rounsaville T.J., Touchell D.H., Ranney T.G., Blazich F.A., 2011. Micro propagation of Mahonia ‘Soft Caress. Hort Science. 46(7), 1010-1014.
18. Sazmand M., Safarnejad A., 2016. Induction of callus and breeding of Berberis vulgaris under in vitro conditions. Journal of Genetic Research and Breeding of Pasture and Forest Plants of Iran. 24(1), 92-101.
19. MezgiNejad Z., 2018. Investigating the in vitro viability of Berberis vulgaris L. callus, Master's thesis in the field of plant breeding. Birjand University- Faculty of Agriculture.
20. Mohammadi M., 2013. In vitro cultivation of seedless barberry (berberis vulgaris var. asperma). Master's thesis. Ferdowsi University of Mashhad - Faculty of Agriculture.
21. Nazari A., 2013. Investigating the relationship between height above sea level and quantitative and qualitative traits in wild species of seeded barberry in Shahrood. Master's thesis in horticulture. Faculty of Agriculture, Islamic Azad University, Damghan branch.
22. Fallahi J., Rizvani Moghadam P., Nasiri Mahalati M., 2010. The effect of harvest date and quantitative and qualitative indicators of Bidaneh rosehip fruit. Iranian Agricultural Research Journal. 8(2), 234-225.
23. Jain A.K., Bashir M., 2010. In Vitro Propagation of a Medicinal Plant Portulaca grandiflora. Hook. World Journal of Agricultural Sciences. 6, 327-330.
24. Nazari A.R, Afshari H., Sinki J.M., Bagheri S., Laei Gh, 2015. The relationship between height above sea level and physiological traits of wild wild barberry (Berberis vulgaris) ecotypes in Shahrood region. Journal of Seedling and Seed Farming. 1(2), 119-126.
25. Agricultural statistics of Iran, 2020. The third volume of garden products.
26. Balandari A., Ziaratnia F., 2012. Investigating the possibility of reproduction of Barberry by the method of tissue culture and rooting of cuttings. Scientific and Industrial Research Organization of Iran - Khorasan Center.
27. Mohammadi M., Bagheri, A., Mushtakhi N., Marashi S.H., Balandari A, Chelvarforosh N., 2011. A review of the effects of browning inhibitory agents and growth regulators on callus induction in seedless barberry (Berberis vulgaris var. asperma), 2nd Iran International Conference on Biotechnology.
28. Sadsi K., 2019. Optimizing micro propagation of Khorasani barberry plant. Master's thesis, Faculty of Humanities, Department of Horticultural Science and Engineering, University of Science and Art affiliated to Academic Jihad.