The Correlation Between Vitamin D Deficiency and Vitamin D Receptor Polymorphisms and The Frequency of Type 2 Diabetes Mellitus Complications Among Egyptian Patients
Subject Areas : Journal of Chemical Health RisksSahar H. Ahmed 1 , Khaled E. Foad 2 , Amer E. Ahmed 3 , Nagla U. Mohamed 4 , Dina Sabry 5
1 - Lab Technology Department, Faculty of Applied Medical Science, Misr University For Science &Technology, Cairo, Egypt
2 - Internal medicine and endocrinology Department, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt
3 - Biotechnology Department, Faculty of Post graduate for Advanced Sciences, Beni-Suef University, Beni-Suef, Egypt
4 - Lab Technology Department, Faculty of Applied Medical Science, Misr University For Science &Technology, Cairo, Egypt
5 - Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt|Medical Biochemistry and Molecular Biology, Faculty of Medicine, Badr University in Cairo, Cairo, Egypt
Keywords: T2DM, Vitamin D deficiency, VDR gene polymorphism,
Abstract :
Diabetes mellitus and obesity are a growing overall medical issues, particularly in developed nations. Vitamin D deficiency is pandemic that has been involved in a different kind of disease. This study was intended to detect the effect of vitamin D deficiency and vitamin D receptor polymorphisms on type 2 diabetes mellitus (T2DM) patients through induction of obesity. 80 Egyptians T2DM patients and 20 non diabetic subjects were included in our research. Diabetic cases were categorized into two subgroups according to diabetic complications. PCR-RFLP for vit D receptor polymorphisms and vit D level were assessed to all subjects. The deficiency of vitamin D was more prevalent in diabetic cases than in control cases. There was significant difference between control and diabetic patients in genotyping of FokI (VDR 2228570 C) and TaqI (VDR rs731236 T) polymorphisms. The results of our study revealed that vitamin D deficiency could be a contributing factor for T2DM rising among Egyptian patients. Additionally, there was a genetic variation in the VDR genes FokI and TaqI related to diabetic mellitus in Egyptian patients.
1. Karpe F., Dickmann J.R., Frayn K.N., 2011. Fatty acids, obesity, and insulin resistance: time for a reevaluation. Diabetes. 60(10), 2441–2449
2. Christakos S., Dhawan P., Verstuyf A., Verlinden L., Carmeliet G., 2016. Vitamin D: Metabolism, Molecular Mechanism of Action, and Pleiotropic Effects. Physiol Rev. 96(1), 365–408.
3. Wacker M., Holick M.F., 2013. Vitamin D—Effects on Skeletal and Extra skeletal Health and the Need for Supplementation. Nutrients. 5(1), 111-148.
4. Bland R., Markovic D., Hills C.E., Hughes S.V., 2004. Expression of 25-hydroxyvitamin D3-1 alpha-hydroxylase in pancreatic islets. J Steroid Biochem Mol Biol. 89-90(1-5), 121-5.
5. Parekh D., Sarathi V., Shivane V.K., Bandgar T.R., Menon P.S., Shah N.S., 2010. Pilot study to evaluate the effect of short-term improvement in vitamin D status on glucose tolerance in patients with type 2 diabetes. Endocr Pract. 16, 600–608.
6. Pittas A.G., Lau J., Hu F.B., Dawson-Hughes B., 2007. The role of vitamin D and calcium in type 2 diabetes: a systematic review and meta-analysis. J Clin Endo Metab. 92(6), 2017–2029.
7. Chang E., Kim Y., 2016. Vitamin D decreases adipocyte lipid storage and increases NAD-SIRT1 pathway in 3T3-L1 adipocytes. Nutrition. 32(6), 702-8.
8. Patrick R.P., Ames B.N., 2014. Vitamin D hormone regulates serotonin synthesis. Part 1: relevance for autism. FASEB J. 28(6), 2398-413.
9. Wang J.H., Keisala T., Solakivi T., Minasyan A., Kalueff A.V., Tuohimaa P., 2009. Serum cholesterol and expression of ApoAI, LXR [beta] and SREBP2 in vitamin D receptor knock-out mice. The J Steroid Biochem. 13(3–5), 222–226.
10. Fleet J.C., DeSmet M., Johnson R., Li Y., 2012. Vitamin D and cancer: a review of molecular mechanisms. Biochem J. 441(1), 61–76.
11. Kostner K., Denzer N., Cornelia S.L., Klein M.R., Tilgen W., Reichrath J., 2009. The Relevance of Vitamin D Receptor (VDR) Gene Polymorphisms for Cancer: A Review of the Literature. Anticancer Research. 29(9), 3511-3536.
12. Harris S.S., Eccleshall T.R., Gross C., Dawson- Hughes B., Feldman D., 1997. The VDR start codon polymorphism (FokI) and bone mineral density in premenopausal American Black and White women. J Bone Miner Res. 12,1043-8.
13. Morrison N.A., Qi J.C., Tokita A., Kelly P.J., Crofts L., Nguyen T.V., Sambrook P.N., Eisman J.A., 1994. Prediction of bone density from Vitamin D receptor alleles. Nature. 367, 284-7.
14. Bayani M.A., Banasaz B., Saeedi F., 2014. Status of Vitamin-D in diabetic patients and compared it with healthy individuals. Caspian J Intern Med 5(1), 40-42.
15. Brijesh M., Saurav P., 2014. Prevalence of vitamin D deficiency in type-2 diabetes mellitus patients and its correlation with glycemic control. International Journal of Bioassays. 3(09) 3313-3317.
16. Shen L., Zhuang Q., Ji H.F., 2016. Assessment of vitamin D levels in type 1 and type 2 diabetes patients: Results from meta-analysis. Mol. Nutr. Food Res. 60(5), 1059–1067.
17. Ozder A., 2014. Lipid profile abnormalities seen in T2DM patients in primary healthcare in Turkey: a cross-sectional study. Lipids Health Disease, 13, 183.
18. Cui R., Qi Z., Zhou L., Li Z., Li Q, Zhang J., 2016. Evaluation of serum lipid profile, body mass index, and waistline in Chinese patients with type 2 diabetes mellitus, Clinical Interventions Aging. 11, 445–452.
19. Dash S., 2017. Correlation of HbA1c with Serum Lipid Profile in Patients with Type 2 Diabetes Mellitus. IOSR Journal of Dental and Medical Sciences. 16(05), 01-04.
20. Akinsegun A., Olusola D.A., Sarah J.O., Olajumoke O., Adewumi A., Majeed O., Anthonia O., Ebele U., Olaitan O., Olanrewaju A., Kingsley A., 2014. Mean platelet volume and platelet counts in type 2 Diabetes: Mellitus on treatment and non-diabetic mellitus controls in Lagos, Nigeria. Pan Afr Med J.18, 42.
21. Chen X., Fang L., Lin H., Shen P., Zhang T., Li H., Li X., Yu M., Xu C., Zhang J., Lu F., DU X., Hu R., Zhong J., 2017. The Relationship between Type 2 Diabetes and platelet indicators. Iran J Public Health. 46(9), 1211-1216.
22. Chuengsamarn S., Rattanamongkolgul S., Jirawatnotai S., 2014. Association between serum uric acid level and microalbuminuria to chronic vascular complications in Thai patients with type 2 diabetes. Journal of Diabetes and Its Complications. 28(2), 124–129.
23. Zhao Q., Laukkanen J.A., Li Q., Li G., 2017. Body mass index is associated with type 2 diabetes mellitus in Chinese elderly. Clinical Interventions in Aging. 2(12), 745–752.
24. Bae J.P., Lage M.J., Mo D., Nelson R., Hoogwerf B.J., 2016. Obesity and glycemic control in patients with diabetes mellitus: Analysis of physician electronic health records in the US from 2009–2011. Journal of Diabetes and Its Complications. 30(2), 212–220.
25. Rawoof A.M., Promela M., Arif A.B., SheikhIshaq, Rabia F., Parveez A.S., Sabhiya M., 2017. Vitamin D Receptor (VDR) Gene Polymorphism and Type 2 Diabetic mellitus (T2DM)-A Case Control Study in Ethnic Population of Kashmir Valley. Adv. Res. Gastroentero. Hepatol. 4(5), 555648.
26. Taneja N., Khadgawat R., Mani S., 2016. BsmI and TaqI polymorphisms in vitamin D receptor gene of type 2 diabetes mellitus patients from North India. Asian J Pharm Clin Res. 9(3), 186-189.
27. Sikander M., 2017. Relationship of ApaI VDR Gene Polymorphism to Type 2 Diabetes Mellitus in Pakistani Population. Electronic Journal of Biology. 13(4), 338-342.
28. Xu J.R., Y. Yang Y., Liu X.M., Wang Y.J., 2014. Association of VDR polymorphisms with type 2 diabetes mellitus in Chinese Han and Hui populations. Genetics and Molecular Research. 13(4), 9588-9598.
29. Angel B., Lera L., Márquez C., Albala C., 2018. The association of VDR polymorphisms and type 2 diabetes in older people living in community in Santiago de Chile. Nutrition and Diabetes. 8(1), 31.
30. AL-Darraji S.Z., AL-Azzawie H.F., AL-Kharsani A.M., 2017. Associations between TaqI Polymorphisms in Vitamin D Receptor Gene and Type 2 Diabetes Mellitus in Obese Iraqi Population. J Diabetes Metab. 8(4), 734.
31. Iyer A.P., New S.L., Khoja S., AL-Ghamdi M.A., Bahlas S., 2017. Association of Apa I polymorphism of vitamin D receptor gene with type 2 diabetes mellitus in Saudi population. Journal of Experimental Biology and Agricultural Sciences. 5(2), 271-276.
32. Mahjoubi I., Kallel A., Hédi Sbaï M., Ftouhi B., Halima M., Jemaa Z., FekiM., Slimane H., Jemaa R., Kaabachi1 N., 2016. Lack of association between FokI polymorphism in vitamin D receptor gene (VDR) & type 2 diabetes mellitus in the Tunisian population. Indian J Med Res. 144(1), 46-51.
33. Errouagui A., Benrahma H., Charoute H., Ghalim N., Barakat A., Kandil M., Rouba H., 2014. Relationship between Vitamin D Receptor (VDR) Gene Polymorphisms and susceptibility to Type 2 Diabetes Mellitus in Moroccans population. International Journal of Innovation and Applied Studies. 8(2), 503-514.