The impact Encapsulation Exerted by Tragacanth Gum on Viability and Staling of Lactobacillus Plantarum and Lactobacillus acidophilus During Baking and Storing Gluten-free Sorghum Bread

Subject Areas : Journal of Chemical Health Risks

Leila  Ghasemi ¹ (Department of Food Science and Technology, Faculty of Agriculture, Islamic Azad University, Damghan Branch, Iran.)
Leila  Nouri ² (Department of Food Science and Technology, Islamic Azad University, Damghan, Iran)
Abdorreza  Mohammadi Nafchi ³ (Department of Food Science and Technology, Damghan Branch, Islamic Azad University, Damghan, Iran|Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia)

Received: 2020-10-24 Accepted : 2022-06-06 Published : 2024-03-01

Keywords: encapsulation, Probiotic, Tragacanth, Gluten-free, Sorghum bread,

Abstract :

Supplementing bread probiotics is considered to be challenging because of the high baking temperatures. In this study the influence of encapsulation by tragacanth gum on the validity of Lactobacillus Plantarum and Lactobacillus acidophilus during baking and storing gluten-free sorghum bread for three days. Moreover, the effect of probiotics encapsulation on moisture content and hardness as two major factors of bread staling were investigated. The process of baking process reduced the observed validity of L. Plantarum and L. acidophilus by about 3 logs CFU/g in gluten-free sorghum bread significantly. Additionally, we found that Plantarum, L. acidophilus, and encapsulated L. during baking and storing processes strongly depend on matrix composition to survive. Encapsulation of probiotic cells by tragacanth gum can improve the viability of probiotic cells can be improved by encapsulating them by more than 2 log cycles in gluten-free sorghum bread during the storing process. The tragacanth gum showed a good protecting impact on L. Plantarum and L. acidophilus cells during 72 h storage. Overall, what the findings suggest is that encapsulating probiotics by tragacanth gum is a strategy promising to promote the survival of bacteria and delay staling of gluten-free sorghum bread.

References:

1. Zhang L., Taal M.A., Boom R.M., Chen X.D., Schutyser M.A., 2018. Effect of baking conditions and storage on the viability of Lactobacillus Plantarum supplemented to bread. LWT. 87, 318-325.


2. Marston K., Khouryieh H., Aramouni F., 2016, Effect of heat treatment of sorghum flour on the functional properties of gluten-free bread and cake. LWT-Food Sci and Tech. 65, 637-644.


3. Schober T.J., Bean S.R., Boyle D.L., 2007. Gluten-free sorghum bread improved by sourdough fermentation: biochemical, rheological, and microstructural background. J of Agri and Food Che. 55(13), 5137-5146.


4. de Souza E.L., de Albuquerque T.M.R., Dos Santos A.S., Massa N.M.L., de Brito Alves J. L., 2019. Potential interactions among phenolic compounds and probiotics for mutual boosting of their health-promoting properties and food functionalities–A review. Critical Reviews in Food Sci and Nutr. 59(10), 1645-1659.


5. Soukoulis C., Yonekura L., Gan H.H., Behboudi-Jobbehdar S., Parmenter C., Fisk I., 2014. Probiotic edible films as a new strategy for developing functional bakery products: The case of pan bread. Food Hyd. 39, 231-242.


6. Yonekura L., Sun H., Soukoulis C., Fisk I., 2014. Microencapsulation of Lactobacillus acidophilus NCIMB 701748 in matrices containing soluble fiber by spray drying: Technological characterization, storage stability, and survival after in vitro digestion. J of Functional Foods. 6, 205-214.


7. Zhang L., Huang S., Ananingsih V.K., Zhou W., Chen X.D., 2014. A study on Bifidobacterium lactic Bb12 viability in bread during baking. J of Food Eng. 122, 33-37.


8. AACC, 2000. Approved methods of the American Association of cereal chemists. American Association of Cereal Chemists. Approved Methods Committee. Vol. 1: Amer Assn of Cereal Chemists.


9. Mudgil D., Barak S., Khatkar B., 2016. Optimization of bread firmness, specific loaf volume, and sensory acceptability of bread with soluble fiber and different water levels. J of Cereal Sci. 70, 186-191.


10. Perdana J., Bereschenko L., Fox M.B., Kuperus J.H., Kleerebezem M., Boom R.M., Schutyser M.A., 2013. Dehydration and thermal inactivation of Lactobacillus Plantarum WCFS1: Comparing single droplet drying to spray and freeze-drying. Food Res Int. 54(2), 1351-1359.


11. Zhang L., Chen X.D., Boom R.M., Schutyser M.A., 2018. Survival of encapsulated Lactobacillus Plantarum during isothermal heating and bread baking. LWT. 93, 396-404.


12. Thang T.D., Hang H.T.T., Luan N.T., KimThuy D.T., Lieu D.M., 2019. Survival Survey of Lactobacillus acidophilus In Additional Probiotic Bread. Turkish Journal of Agriculture-Food Sci and Tech. 7(4), 588-592.


13. Trabelsi I., Ayadi D., Bejar W., Bejar S., Chouayekh H., Salah R.B., 2014. Effects of Lactobacillus Plantarum immobilization in alginate coated with chitosan and gelatin on antibacterial activity. Int J of Bio Mac. 64, 84-89.


14. Rosell C.M., Rojas J.A., De Barber C.B., 2001. Influence of hydrocolloids on dough rheology and bread quality. Food Hyd. 15(1), 75-81.


15. Bernela M., Ahuja M., Thakur R., 2016. Enhancement of anti-inflammatory activity of glycyrrhizic acid by encapsulation in chitosan-katara gum nanoparticles. Eur J of Pharma and Bio. 105, 141-147.


16. Guarda A., Rosell C.M., Benedito C., Galotto M.J., 2004. Different hydrocolloids as bread improvers and antistaling agents. Food Hyd. 18(2), 241-247.


17. Collar C., Martinez J., Rosell C., 2001. Lipid binding of fresh and stored formulated wheat bread. Relationships with dough and bread technological performance. Food Sci and Tech Int. 7(6), 501-510.


18. Tebben L. Li Y., 2019. Effect of xanthan gum on dough properties and bread qualities made from whole wheat flour. Cereal Chem. 96(2), 263-272.


19. Mandala I., 2005. Physical properties of fresh and frozen stored, microwave-reheated bread, containing hydrocolloids. J of Food Eng. 66(3), 291-300.


20. Shittu T.A., Aminu R.A., Abulude E.O., 2009. Functional effects of xanthan gum on composite cassava-wheat dough and bread. Food Hyd. 23(8), 2254-2260.