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Manuscript ID : JCHR-2107-1364 (R1) Visit : 137 Page: 671 - 682

10.22034/jchr.2022.1935870.1364

Article Type: Original Research

The Impact of Artemisia absinthium Hydro-ethanolic Extract on Oxidative Stress in the Brain in a Seizure Model Induced by Pentylenetetrazole in Mice

Subject Areas : Journal of Chemical Health Risks

Roghayeh  Rashidi 1 (Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran)
Mohammad Reza  Mahdinezhad 2 (Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran)
Mohammad-Ali  Sobhanifar 3 (Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran)
Narges  Marefati 4 (Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran)
Hassan  Rakhshandeh 5 (Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran)
Mahmoud  Hosseini 6 (Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran|Neuroscience Research Center, Mashhad University of Medical Sciences, Mashhad, Iran)

Received: 2021-07-18 Accepted : 2022-07-25 Published : 2023-12-01

Keywords: Oxidative stress, epilepsy, Artemisia absinthium, Pentylenetetrazole,

Abstract :

Oxidative stress has a crucial role in epileptic seizures. Several studies have shown the protective effect of Artemisia absinthium (A. absinthium) against neuronal damage and oxidative stress. In the current research, the effect of A. absinthium on oxidative stress indicators in an animal model of seizure provoked by injecting pentylenetetrazole (PTZ) was estimated in mice. The mice were allocated into the following groups: a control group in which vehicle was administered; PTZ group (a single dose of 100 mg kg-1, ip); and other groups, which daily received 6.25-200 mg kg-1 of the A. absinthium extract during 3 consecutive days before PTZ. The first recorded MCS and minimal clonic seizure and the first generalized tonic-clonic seizure (GTCS) latencies were analyzed. The brain segments, including the cortex and hippocampus of the animals, were then removed and harvested for oxidative stress evaluation. The extract significantly postponed the onsets of the MCS and GTCS when injected before PTZ. The seizure attacks provoked by PTZ also increased MDA in the cortex and hippocampus to levels greater than the control (P<0.001). In addition, the extract had an ameliorative effect on MDA concentration in the cortex and hippocampus (P<0.05-P<0.001). A lower concentration of total thiol was observed in the brain of the PTZ injected mice than the control ones (P<0.01- P<0.001). Pretreatment with the extract corrected the thiol level in the brain tissue (P<0.05- P<0.001). The current research shows that A. absinthium hydro-ethanolic extract has considerable anti-oxidant properties in a PTZ-induced seizure model in mice.

References:


  1. Pourzaki M., Homayoun M., Sadeghi S., Seghatoleslam M., Hosseini M., Ebrahimzadeh Bideskan A., 2017. Preventive effect of Coriandrum sativum on neuronal damages in pentylentetrazole-induced seizure in rats. Avicenna J Phytomed.7(2), 116-128.
    2.
  2. Sander J.W., 2003. The epidemiology of epilepsy revisited. Current Opinion in Neurology. 16(2), 165-170.
    3.
  3. Meador K.J., 2002. Cognitive outcomes and predictive factors in epilepsy. Neurology. 58(8 suppl 5), 21-26.
    4.
  4. Kudin A.P., Kudina T.A., Seyfried J., Vielhaber S., Beck H., Elger C.E., Kunz W.S., 2002. Seizure‐dependent modulation of mitochondrial oxidative phosphorylation in rat hippocampus. European Journal of Neuroscience. 15(7), 1105-1114.
    5.
  5. Mehla J., Reeta K., Gupta P., Gupta Y. K., 2010. Protective effect of curcumin against seizures and cognitive impairment in a pentylenetetrazole-kindled epileptic rat model. Life Sciences. 87(19-22), 596-603.
    6.
  6. Hosseini M., Harandizadeh F., Niazamand S., Soukhtanloo M., Mahmoudabady M., 2013. Antioxidant effect of Achillea wilhelmsii extract on pentylenetetrazole (seizure model)-induced oxidative brain damage in Wistar rats. Indian J Physiol Pharmacol. 57(4), 418-424.
    7.
  7. Rösche J., Uhlmann C., Fröscher W., 2010. Cognitive deficits and psychiatric disorders in patients with new-onset epilepsy. Fortschritte der Neurologie-Psychiatrie. 78(1), 18-26.
    8.
  8. Gupta Y.K., Briyal S., 2006. Protective effect of vineatrol against kainic acid induced seizures, oxidative stress and on the expression of heat shock proteins in rats. European Neuropsychopharmacology. 16(2), 85-91.
    9.
  9. Ramesh K.V., Padmavathi K., 2010. Assessment of immunomodulatory activity of Euphorbia hirta L. Indian Journal of Pharmaceutical Sciences. 72(5), 621-625.
    10.
  10. Rakhshandah H., Hosseini M., 2006. Potentiation of pentobarbital hypnosis by Rosa damascena in mice. Indian Journal of Experimental Biology. 44(11), 910-912.
    11.
  11. Rakhshandeh H., Heidari A., Pourbagher-Shahri A.M., Rashidi R., Forouzanfar F., 2021. Hypnotic Effect of A. absinthium Hydroalcoholic Extract in Pentobarbital-Treated Mice. Neurology Research International. 21, 2021.
    12.
  12. Ueda J., Kato J., 1980. Isolation and identification of a senescence-promoting substance from wormwood (Artemisia absinthium L.). Plant Physiology. 66(2), 246-249.
    13.
  13. Wake G., Court J., Pickering A., Lewis R., Wilkins R., Perry E., 2000. CNS acetylcholine receptor activity in European medicinal plants traditionally used to improve failing memory. Journal of Ethnopharmacology. 69(2), 105-114.
    14.
  14. Guarrera P.M., 2005. Traditional phytotherapy in Central Italy (Marche, Abruzzo, and Latium). Fitoterapia. 76(1), 1-25.
    15.
  15. Mahmoudi M., Ebrahimzadeh M., Ansaroudi F., Nabavi S., Nabavi S., 2009. Antidepressant and antioxidant activities of Artemisia absinthium L. at flowering stage. African journal of Biotechnology. 8(24), 7170-7175.
    16.
  16. Bora K.S., Sharma A., 2010. Neuroprotective effect of Artemisia absinthium L. on focal ischemia and reperfusion-induced cerebral injury. Journal of Ethnopharmacology. 129(3), 403-409.
    17.
  17. Oliveira J.S., Porto L.A., Estevam C.D.S., Siqueira R.D.S., Barreto P.B., Niculau E.D.S., Blank A.F., Almeida R.N.D., Marchioro M., Quintans-Júnior L.J., 2009. Phytochemical screening and anticonvulsant property of Ocimum basilicum leaf essential oil. Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas. 8(3), 195-202.
    18.
  18. Rezaeinodehi A., Khangholi S., 2008. Chemical composition of the essential oil of Artemisia absinthium growing wild in Iran. Pak J Biol Sci. 11(6), 946-949.
    19.
  19. Guzmán-Gutiérrez S.L., Bonilla-Jaime H., Gómez-Cansino R., Reyes-Chilpa R., 2015. Linalool and β-pinene exert their antidepressant-like activity through the monoaminergic pathway. Life Sciences. 128, 24-29.
    20.
  20. Ekiert H., Knut E., Świątkowska J., Klin P., Rzepiela A., Tomczyk M., 2021. Artemisia abrotanum L. (Southern Wormwood)-History, Current Knowledge on the Chemistry, Biological Activity, Traditional Use and Possible New Pharmaceutical and Cosmetological Applications. Molecules. 26(9), 2503-2523.
    21.
  21. Rajabian A., Hosseini A., Hosseini M., Sadeghnia H.R., 2019. A Review of Potential Efficacy of Saffron (Crocus sativus L.) in Cognitive Dysfunction and Seizures. Prev Nutr Food Sci. 24(4), 363-372.
    22.
  22. Baradaran Rahimi V., Askari V.R., Hosseini M., Yousefsani B.S., Sadeghnia H.R., 2019. Anticonvulsant Activity of Viola tricolor against Seizures Induced by Pentylenetetrazol and Maximal Electroshock in Mice. Iran J Med Sci. 44(3), 220-226.
    23.
  23. Seghatoleslam M., Alipour F., Shafieian R., Hassanzadeh Z., Edalatmanesh M.A., Sadeghnia H.R., Hosseini M., 2016. The effects of Nigella sativa on neural damage after pentylenetetrazole induced seizures in rats. J Tradit Complement Med. 6(3), 262-268.
    24.
  24. Babakhani B., Janbaz F., Ebrahimzadeh M.A., 2018. Influence of different extraction methods on antioxidant and antibacterial activities of Nonnea lutea. Journal of Mazandaran University of Medical Sciences. 27(156), 129-145.
    25.
  25. Shahnazi R., Mehrdadfar F., Ebrahimzadeh M. A., 2018. Impact of extraction methods on total phenolic and flavonoid contents, antioxidant and antihypoxic properties of Allium ampeloprasum in Mice. Journal of Mazandaran University of Medical Sciences. 27(158), 27-44.
    26.
  26. Marefati N., Mokhtari-Zaer A., Beheshti F., Karimi S., Mahdian Z., Khodamoradi M., Hosseini M., 2019. The effects of soy on scopolamine-induced spatial learning and memory impairments are comparable to the effects of estradiol. Horm Mol Biol Clin Investig. 39(3), 12.
    27.
  27. Karami R., Hosseini M., Mohammadpour T., Ghorbani A., Sadeghnia H. R., Rakhshandeh H., Vafaee F., Esmaeilizadeh M., 2015. Effects of hydroalcoholic extract of Coriandrum sativum on oxidative damage in pentylenetetrazole-induced seizures in rats. Iranian Journal of Neurology. 14(2), 59-66.
    28.
  28. Khodabakhshi T., Beheshti F., Hosseini M., Mousavi S.M., Rakhshandeh H., Sadeghnia H.R., Aghaei A., 2017. Effect of Ocimum basilicum hydro-alcoholic extract on oxidative damage of brain tissue following seizures induced by pentylenetetrazole in mice. Physiology and Pharmacology. 21(4), 295-303.
    29.
  29. Ebrahimzadeh A.B., Hosseini M., Mohammadpour T., Karami R., Khodamoradi M., Nemati H.K., Alavi H., 2011. Effects of soy extract on pentylenetetrazol-induced seizures in ovariectomized rats. Journal of Chinese Integrative Medicine. 9(6), 611-618.
    30.
  30. Hosseini M., Sadeghnia H.R., Salehabadi S., Alavi H., Gorji A., 2009. The effect of L-arginine and L-NAME on pentylenetetrazole induced seizures in ovariectomized rats, an in vivo study. Seizure. 18(10), 695-698.
    31.
  31. Hosseini M., Harandizadeh F., Niazmand S., Soukhtanloo M., Faizpour A., Ghasemabady M., 2014. The role for nitric oxide on the effects of hydroalcoholic extract of Achillea wilhelmsii on seizure. Avicenna Journal of Phytomedicine. 4(4), 251-259.
    32.
  32. Hosseini M., Pkan P., Rakhshandeh H., Aghaie A., Sadeghnia H.R., Ghasemzadeh Rahbardar M., 2011. The effect of hydro-alcoholic extract of citrus flower on pentylenetetrazole and maximal electroshock-induced seizures in mice. World Applied Sciences Journal. 15(8), 1104-1109.
    33.
  33. Hosseini M., Pourganji M., Khodabandehloo F., Soukhtanloo M., Zabihi H., 2012. Protective effect of l-arginine against oxidative damage as a possible mechanism of its Bene. cial properties on spatial learning in ovariectomized rats. Basic and Clinical Neuroscience. 3(5), 36-44.
    34.
  34. Vafaee F., Hosseini M., Sadeghinia H.R., Hadjzadeh M.A.R., Soukhtanloo M., Rahimi M., 2014. The effects of soy extract on spatial learning and memory damage induced by global ischemia in ovariectomised rats. The Malaysian Journal of Medical Sciences: MJMS. 21(3), 19-30.
    35.
  35. Pourganji M., Hosseini M., Soukhtanloo M., Zabihi H., Hadjzadeh M.A.R., 2014. Protective role of endogenous ovarian hormones against learning and memory impairments and brain tissues oxidative damage induced by lipopolysaccharide. Iranian Red Crescent Medical Journal. 16(3), 13954-13962.
    36.
  36. Helmstaedter C., Sadat-Hossieny Z., Kanner A.M., Meador K.J., 2020. Cognitive disorders in epilepsy II: Clinical targets, indications and selection of test instruments. Seizure. 83, 223-231.
    37.
  37. Kanner A.M., Helmstaedter C., Sadat-Hossieny Z., Meador K., 2020. Cognitive disorders in epilepsy I: Clinical experience, real-world evidence and recommendations. Seizure. 83, 216-222.
    38.
  38. Vafaee F., Hosseini M., Hassanzadeh Z., Edalatmanesh M.A., Sadeghnia H.R., Seghatoleslam M., Mousavi S.M., Amani A., Shafei M.N., 2015. The Effects of Nigella Sativa Hydro-alcoholic Extract on Memory and Brain Tissues Oxidative Damage after Repeated Seizures in Rats. Iran J Pharm Res. 14(2), 547-557.
    39.
  39. Ebrahimzadeh-Bideskan A.R., Mansouri S., Ataei M.L., Jahanshahi M., Hosseini M., 2018. The effects of soy and tamoxifen on apoptosis in the hippocampus and dentate gyrus in a pentylenetetrazole-induced seizure model of ovariectomized rats. Anat Sci Int. 93(2), 218-230.
    40.
  40. Homayoun M., Shafieian R., Seghatoleslam M., Hosseini M., Ebrahimzadeh-Bideskan A., 2020. Protective impact of Rosa damascena against neural damage in a rat model of pentylenetetrazole (PTZ)-induced seizure. Avicenna J Phytomed. 10(6), 574-583.
    41.
  41. Anaeigoudari A., Hosseini M., Karami R., Vafaee F., Mohammadpour T., Ghorbani A., Sadeghnia H. R., 2016. The effects of different fractions of Coriandrum sativum on pentylenetetrazole-induced seizures and brain tissues oxidative damage in rats. Avicenna J Phytomed. 6(2), 223-235.
    42.
  42. Akyuz E., Kullu I., Arulsamy A., 2021. Melatonin as an Antiepileptic Molecule: Therapeutic Implications via Neuroprotective and Inflammatory Mechanisms. ACS Chemical Neuroscience. 12(8), 1281-92.
    43.
  43. Ilhan A., Aladag M.A., Kocer A., Boluk A., Gurel A., Armutcu F., 2005. Erdosteine ameliorates PTZ-induced oxidative stress in mice seizure model. Brain Research Bulletin. 65(6), 495-499.
    44.
  44. Zhu X., Dong J., Han B., Huang R., Zhang A., Xia Z., Chang H., Chao J., Yao H., 2017. Neuronal nitric oxide synthase contributes to PTZ kindling epilepsy-induced hippocampal endoplasmic reticulum stress and oxidative damage. Frontiers in Cellular Neuroscience. 11, 377-393.
    45.
  45. Choopankareh S., Vafaee F., Shafei M.N., Sadeghnia H.R., Salarinia R., Zarepoor L., Hosseini M., 2015. Effects of melatonin and theanine administration on pentylenetetrazole-induced seizures and brain tissue oxidative damage in ovariectomized rats. Turk J Med Sci. 45(4), 842-849.
    46.
  46. He L. ., Hu M.B., Li R.L., Zhao R., Fan L.H., He L., Lu F., Ye X., Huang Y.L., Wu C.J., 2021. Natural Medicines for the Treatment of Epilepsy: Bioactive Components, Pharmacology and Mechanism. Front Pharmacol. 12, 604040-604068.
    47.
  47. Yang N., Guan Q.W., Chen F.H., Xia Q.X., Yin X.X., Zhou H.H., Mao X.Y., 2020. Antioxidants Targeting Mitochondrial Oxidative Stress: Promising Neuroprotectants for Epilepsy. 6687185-6687199.
    48.
  48. Golechha M., Bhatia J., Arya D.S., 2010. Hydroalcoholic extract of Emblica officinalis Gaertn. affords protection against PTZ-induced seizures, oxidative stress and cognitive impairment in rats. Indian Journal of Experimental Biology. 48(5), 474-478.
    49.
  49. Ilhan A., Gurel A., Armutcu F., Kamisli S., Iraz M., 2005. Antiepileptogenic and antioxidant effects of Nigella sativa oil against pentylenetetrazol-induced kindling in mice. Neuropharmacology. 49(4), 456-464.
    50.

 

 

 

  1. Szopa A., Pajor J., Klin P., Rzepiela A., Elansary H.O., 2020. Artemisia absinthium L.-Importance in the History of Medicine, the Latest Advances in Phytochemistry and Therapeutical, Cosmetological and Culinary Uses. Plants. 9(9), 1063-1096.
    2.
  2. Zeng K.W., Liao L.X., Song X.M., Lv H.N., Song F.J., Yu Q., Dong X., Jiang Y., Tu P.F., 2015. Caruifolin D from Artemisia absinthium L. inhibits neuroinflammation via reactive oxygen species-dependent c-jun N-terminal kinase and protein kinase c/NF-κB signaling pathways. European Journal of Pharmacology. 767, 82-93.
    3.
  3. Kharoubi O., Slimani M., Aoues A., 2011. Neuroprotective effect of wormwood against lead exposure. Journal of Emergencies, Trauma and Shock. 4(1), 82-88.
    4.
  4. Amat N., Upur H., Blažeković B., 2010. In vivo hepatoprotective activity of the aqueous extract of Artemisia absinthium L. against chemically and immunologically induced liver injuries in mice. Journal of Ethnopharmacology. 131(2), 478-484.
    5.
  5. Bora K.S., Sharma A., 2011. Evaluation of antioxidant and free-radical scavenging potential of Artemisia absinthium. Pharmaceutical Biology. 49(12), 1216-1223.
    6.
  6. Wani H., Shah S.A., Banday J.A., 2014. Chemical composition and antioxidant activity of the leaf essential oil of Artemisia absinthium growing wild in Kashmir, India. Aust J Pharm. 3(2), 90-94.
    7.
  7. Hallal N., Kharoubi O., Benyettou I., Tair K., Ozaslan M., Aoues A., 2016. In vivo amelioration of oxidative stress by Artemisia absinthium L. administration on mercuric chloride toxicity in brain regions. J Biol Sci. 16, 167-177.
    8.
  8. Al-Malki A.L., 2019. Shikimic acid from Artemisia absinthium inhibits protein glycation in diabetic rats. International Journal of Biological Macromolecules. 122, 1212-1216.
    9.

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