Investigating the Effect of Eugenol on Regulating the Inflammatory Pathways under the Control of miR-223-3p in the Glioblastoma

Subject Areas : Journal of Chemical Health Risks

Nasrollah Naghdi ¹ , Mahsa Asadi ² , Mehdi Safari ³ , Babak Gholamine ^{4 *} , Fakhriyeh Mazalzadeh ⁵

1 - Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 - Research Committee of Dezful University of Medical Sciences, Dezful, Iran
3 - Assistant Professor Department of Health in Disasters and Emergencies, School of Public Health and Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 - Department of Pharmacology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 - Nursing and Midwifery School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Received: 2024-11-23 Accepted : 2025-04-28 Published : 2025-06-15

Keywords: Eugenol, Glioblastoma, miR-223-3p, Marker, Treatment,

Abstract :

MicroRNA-223 (miR-223) has become a significant regulator in various biological processes, particularly in gliomas and inflammatory responses. Research indicates that miR-223 is crucial in modulating drug resistance in glioma stem cells (GSCs), particularly in response to temozolomide (TMZ) treatment. Elevated levels of miR-223 correlate with increased chemoresistance, linked to the downregulation of PAX6 and activation of the PI3K/AKT signaling pathway. Conversely, overexpression of miR-223 enhances the sensitivity of glioblastoma (GBM) cells to radiation-induced apoptosis, suggesting it is an impending therapeutic target. Additionally, miR-223 is connected with epithelial-mesenchymal transition (EMT) in GBM, promoting invasive properties and correlating with the expression of metalloproteinases and vascular endothelial growth factor (VEGFA). In the context of inflammation, miR-223 regulates immune cell functions by targeting NLRP3, a key component of the inflammasome, thereby influencing neutrophil and macrophage activity. Its role in mitigating acute lung injury and liver failure by inhibiting NET formation further underscores its importance in inflammatory diseases. Overall, miR-223 represents a dual-function molecule with implications in cancer progression and inflammatory responses, highlighting its potential as a biomarker and healing objective in gliomas and related conditions. Overall, the findings suggest that both eugenol and miR-223 play significant roles in glioblastoma biology, offering avenues for further research and potential therapeutic interventions.

 

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