The Effect of Hydroalcoholic Extract of Berberis vulgaris on Wound Healing of Diabetic Wistar Rats

Subject Areas : Journal of Chemical Health Risks

Mansoureh Pashaee ¹ , Abdolhossein Shiravi ² , Vida Hojati ³

1 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran

Received: 2016-09-17 Accepted : 2018-10-29 Published : 2016-09-17

Keywords: Rats, Diabetes, Barberry, Skin lesions,

Abstract :

Diabetes mellitus is one of the most common diseases of the endocrine system. Barberry (Berberis vulgaris), as a common herb incorporated in treatment of various diseases, is used as anti-itch and anti-microbial medicine as well as lowering blood sugar and blood pressure. In this study, the effects of a hydroalcoholic extract of B. vulgaris on skin wound healing of diabetic rats were investigated. Forty eight male Wistar rats weighting 180 to 230 g were divided into four groups: control group (non-treated non-diabetic rats), sham group (oserin-treated diabetic rats), experimental 1 group (non-treated diabetic rats), experimental 2 group (B. vulgaris extract-treated diabetic rats) and then, 3-cm-length cuts were created on the skin of the dorsal lumbar region and then the wounds were treated on a daily basis. Lesion length measurements were conducted during the experiments using a caliper. For the purpose of histological studies, wound samples were taken from each group and finally, statistical data analysis was conducted using the one-way ANOVA test in addition to the Duncan test performed using the SPSS software. The results indicated that the second experimental group had the best influence on wound healing compared to others. Accordingly, the B. vulgaris extract might be able to improve the wound healing process along with reducing the lesion length due to its anti-diabetic and antimicrobial effects in rats after 21 days.

References:

1. Nakamura U., Iwase M., Uchizono Y., Sonoki K., Sasaki N., Imoto H., Goto D., Iida M., 2006. Rapid intracellular acidification and cell death by H2O2 and alloxan in pancreatic beta cells. Free Radic Biol Med. 40(11), 2047-2055.
2. Tripathi B.K., Srivastava A.K., 2006. Diabetes mellitus: complications and therapeutics. Med Sci Monit. 12, 130-47.
3. Soleimani M., Asgari M.R., Babamohammadi H., Nobahar M., Vafaee A.A. 2006. Comprehensive Textbook of Medical-Surgical (Bruner, Lakman and Fyps). Bashari. First Edition.
4. Rojhan M.M. 2006. Herbal Medicine. Educational organizations. Alavi Publication.
5. Forghani B., 1995. Barberry in the treatment of diabetes. The 1st Conference of Process of Barberry. Birjand. 11-12 November.
6. Sharifi H., Rahmanpour M., Dadravesh D., 2012. A review of effective herbal medicines for high blood pressure. Bojnourd, North Khorasan University of Medical Sciences.
7. Konczak I., Zhang W., 2004. Anthocyanins-More than nature's colours. J Biomed Biotechnol. 5, 239-240.
8. Zhang Q., Cai L., Zhong G., Luo W., 2010. Simultaneous determination of jatrorrhizine, palmatine, berberine, and obacunone in Phellodendri Amurensis Cortex by RP-HPLC. Zhongguo Zhong Yao Za Zhi. 35(16), 2061-2064.
9. Kuo C.L., Chi C.W., Liu T.Y., 2004. The anti-inflammatory potential of berberine in vitro and in vivo. Cancer Lett. 203(2), 127-137.
10. Gürbüz I., Akyüz C., Yeşilada E., Şener B., 2000. Antiulcerogenic effect of Momordica charantia L. fruits on various ulcer models in rats. J Ethnopharmacol. 71, 77-82.
11. Handa S.S., Chawla A.S., Maninder S., 1989. Hypoglycemic plants- review. Fitotrapia. 60, 195-202.
12. Asuzu I.U., Onu O.U., 1990. Anti-ulcer activity of the ethanolic extract of Combretum dolichopetalum root. Int J Crude Drug Res. 28(1), 27-32.
13. Boisnic S., 2005. Healing effect of spary containing Rhealba oat colloidal extract in an invitro recons reconstitution model of skin. Tissue React. 27(3), 83-89.
14. Chevallier A., 2001. The encyclopedia of medicinal plants. 2nd ed., London: Dorling Kindersley.
15. Chen M., Christensen S.B., Theander T.G., Kharazmi A., 1994. Antileishmanial activity of licochalcone A in mice infected with Leishmania major and in hamsters infected with Leishmania donovani. Antimicrob Agents Chemother. 38(6), 1339-1344.
16. Mortazavi A., Mirzababaiee S, Elhami Rad A., 2001. Schematic design process and processing of barberry. Industrial Research Organization of Iran, Khorasan Center.
17. Khorshidzadeh F., 1984. Review in the natural colors and color combinations in barberry plant. DVM Thesis of Tehran University.
18. Kumar B., Vijayakumar M., Govindarajan R., Pushpangadan P., 2007. Ethnopharmacological approaches to wound healing exploring medicinal plants of India. J Ethnopharmacol. 114(2), 103-113.
19. Beutler E., Kelly B.M., 1963. The effect of sodium nitrate on red cell glutathione. Experientia. 19, 96-97.
20. Dweck A.C., 2002. Herbal medicine for the skin. Their chemistry and effects on skin and mucous membranes. Pers Care Mag. 3(2), 19-21.
21. Wang C., Li J., Lv X., Zhang M., Song Y., Chen L., 2009. Ameliorative effect of berberine on endothelial dysfunction in diabetic rats induced by high fat diet and streptozotocin. Eur J Pharmacol. 620(1ââ‚‌“3), 131-137.
22. Wang Y, Campbell T, Perry B, Beaurepaire C, Qin L. 2010. Hypoglycemic and insulin-sensitizing effects of berberine in high-fat diet- and streptozotocin-induced diabetic rats. Metabolism. 60(2), 298-305.
23. Yin J, Gao Z, Liu D, Liu Z, Ye J. 2008. Berberine improves glucose metabolism through induction of glycolysis. Am J Physiol Endocrinol Metab. 294(1), 148-156.
24. Wu L.Y., Ma Z.M., Fan X.L., Zhao T., Liu Z.H., Huang X., Li M.M., Xiong L., Zhang K., Zhu L. L., Fan M., 2010. The anti-necrosis role of hypoxic preconditioning after acute anoxia is mediated by aldose reductase and sorbitol pathway in PC12 cells. Cell Stress Chaperones. 15(4), 387-394.
25. Kong W.J., Zhang H., Song D.Q., Xue R., Zhao W., Wei J., 2009. Berberine reduces insulin resistance through protein kinase C-dependent up-regulation of insulin receptor expression. Metabolism. 58(1), 109-119.
26. Yin J., Xing H., Ye J., 2008. Efficacy of berberine in patients with type 2 diabetes mellitus. Metabolism. 57(5), 712-717.
27. Zhang H., Wei J., Xue R., Wu J.D., Zhao W., Wang Z.Z., Wang S.K., Zhou Z.X., Song D.Q., Wang Y.M., Pan H.N., Kong W.J., Jiang J.D., 2010. Berberine lowers blood glucose in type 2 diabetes mellitus patients through increasing insulin receptor expression. Metabolism. 59(2), 285-292.
28. Lu S.S., Yu Y.L., Zhu H.J., Liu X.D., Liu L., Liu Y.W., Wang P., Xie L., Wang G.J., 2009. Berberine promotes glucagon-like peptide-1 (7-36) amide secretion in streptozotocin-induced diabetic rats. J Endocrinol. 200(2), 159-165.