Assessment Bioremediation of Contaminated Soils to Petroleum Compounds and Role of Chemical Fertilizers in the Decomposition Process
Subject Areas : Journal of Chemical Health RisksH. Parvizi Mosaed 1 , S. Sobhan Ardakani 2 , M. Cheraghi 3
1 - Young Researchers & Elites Club, Hamedan Branch, Islamic Azad University, Hamedan, Iran
2 - Department of the Environment, Hamedan Branch, Islamic Azad University, Hamedan, Iran
3 - Department of the Environment, Hamedan Branch, Islamic Azad University, Hamedan, Iran
Keywords: soil, microorganisms, Bioremediation, petroleum hydrocarbons, chemical fertilizers,
Abstract :
Today oil removal from contaminated soil by new methods such as bioremediation is necessary. In this paper, the effect of chemical fertilizers and aeration on bioremediation of oil-contaminated soil has been investigated. Also the control group, (bioremediation of petroleum hydrocarbons in contaminated soil without treatment by chemical fertilizers and aeration treatment was examined. The condition of experiment is as following: those were treated 70 days in glass columns (30×30×30cm dimensions), ambient temperature (25-30 0C), relative humidity 70%, aeration operation with flow 0.7 lit/min. The total number of heterotrophic bacteria of break down oil and the total of petroleum hydrocarbons were analyzed using gas chromatography analysis. all experiments were replicated three times. The microbial population results for control soil, treated soil by aeration and treated soil by aeration and chemical fertilizers columns are 2.3×105, 1.04×1010, and 1.14×1011 CFU/gr, respectively. The concentrations of total petroleum hydrocarbons of remaining are 46965, 38124, and 22187 mg kg-1respectively. The obtained results show that the aeration operation and chemical fertilizers have effective role on degradation of petroleum hydrocarbon by oil degrading bacteria from soil.
- Norris R. D., Hinchee R. E., Brown R. A.,
- McCarty P. L., Semprini L., Wilson J. T.,
- Kampbell D. H., Reinhard M., Bower E. J.,
- Borden R. C., Vogel T. M., Thomas J. M., Ward
- C. H., 1994. Handbook of Bioremediation. Boca
- Raton, FL:CRC Press.
- U.S. Environmental Protection Agency
- (EPA).,1991. Soil Vapor Extraction Technology:
- Reference Handbook. Cincinnati, OH: Office of
- Research and Development. EPA/540/2-9.
- Frick C. M., Farrell R. E., Germida J. J., 1999.
- Assessment of Phytoremediation as an in situ
- technique for cleaning oil-contaminated sites,
- Petroleum Technology Alliance Canada, Calgary.
- Lundstedt S., 2003. Analysis of PAHs and their
- transformation products in contaminated soil and
- remedial processes, Solfjodern Offset AB, Umea,
- Sweden, pp: 55.
- Mills M. A., Bonner J. S., McDonald T. J., Paqe C.
- A., Autenrieth R. L., 2003. Intrinsic
- bioremediation of a petroleum impacted wetland.
- Marine Pollutin Bulletin, 46, pp 887-899.
- Chorom M., Sharifi H. S., Motamedi H., 2010.
- Bioremediation of a crude oil-polluted soil by
- application of fertilizers, Iran. J. Environ. Health.
- Sci. En, Vol. 7, No. 4, pp: 319-326.
- Prince R. C., Lessard R. R., Clark R. ,2003.
- Bioremediation of marine oil spills, Oil & Gas and
- Technology-Rev, 58 (4), 463-468.
- Mendelssohn I. A., Lin Q., 2003. Development of
- bioremediation for oil spill cleanup in costal
- wetland. OCS Study, MMS 2002-2048.
- Rittmann B. E., McCarty P. L., 2001.
- Enviromental Biotechnology: Principles and
- Applications, McGraw-Hill, New York, p. 695.
- Tang J., Niu X., Sun Q., Wang R., 2010.
- Bioremediation of Petroleum Polluted Soil by
- Combination of Ryegrass with Effective
- Microorganisms, Journal of Environmental
- Technology and Engineering, 3(2) : pp 80-86.
- Walworth J. L., Reynolds C. M., 1995.
- Bioremediation of a petroleum-contaminated cryic
- soil: effects of Phosphorous, nitrogen, and
- temperature. Journal of Soil Contam, 4:pp 299 -
- Facundo J., Marquez R., Hernandez V., Teresa
- Lamela M. A., 2000. Biodegraradation of diesel
- oil in soil by a microbial consortium, pp: 313-320.
- Milic J. S., Beskoski V. P., Ilic M.V., Ali S. M.,
- GojgicCvijovic G. D., Vrvic M. M.,
- Bioremediation of soil heavily contaminated
- with crude oil and its products: composition of the
- microbial consortium, J. Serb. Chem. Soc. 74 (4):
- pp 455âââ460.
- Osterreicher-Cunha P., Do Amaral Vargas Jr. E.,
- Remy Davee Guimaraes J., Mauro Pereira de
- Campos T., Maria Ferreira Nunes C., Costa A.,
- Dos Santos Antunes F., Isabel Pais da Silva M.,
- Maria Mano D., 2004. Evaluation of bioventing on
- a gasolineâââethanol contaminated undisturbed
- residual soil, Journal of Hazardous Materials, Vol.
- , pp 64-66.
- Schaefer M., Juliane F., 2007. The influence of
- earthworms and organic additives on the
- biodegradation of oil contaminated soil. Applied
- Soil Ecoogy, 36, pp: 53-62.
- USDA., 1993. ââÅSoil Survey Manual, United States
- Department of AgricultureââÂ, USA.
- AOAC (Association of Official Analytical
- Chemists), 2005. Methods of Analysis.,
- Washington D.C.
- Klute A. (ed.)., 1986. Methods of Soil
- Analysis.Part 1, 2nd ed. American Society of
- Agronomy and Soil Science Society of America,
- Madison, WI.
- Wrenn B. A., Venosa A. D., 1996. ââÅSelective
- enumeration of aromatic and aliphatic
- hydrocarbon degrading bacteria by a mostprobable-
- number procedureââÂ. Canadian Journal of
- Microbiology: 42 (2) :pp252âââ258.
- Gogoi B. K., Dutta N. N., Goswami P., Krishna
- Mohan T. R., 2003. A case study of
- bioremediation of petroleumhydrocarbon
- contaminated soil at a crude oil spill site.
- Advances in Environmental Research, 7: pp 767-
- Christopher S., Hein P., Marsden J., Shurleff A. S.,
- Evaluation of methods 3540 (soxhlet) and
- (Sonication) for evaluation of appendix IX
- analyses from solid samples. S-CUBED, Report
- for EPA contract 68-03-33-75, work assignment
- No.03, Document No. SSS-R-88-9436.
- U. S. EPA., 1984. Interalaboratory Comparison
- Stunt: Methods for volatile and semiâââvolatile
- compounds, Environmental monitoring systems
- laboratory. Office of Research and Development,
- Las Vegas, NV, EPA. 600/4- 84- 027.
- Kang W., Cheong J., Kim K., Chang J., 2011a.
- Remediation of TPH-Contaminated Soils in the
- Railroad Area by Using Soil Washing. Proc. Of
- Korea Society of Environmental Engineers., pp:
- -103.
- Kang W., Cheong J., Kim K., Chang J. Kim D.,
- b. Remediation of Petroleum-Contaminated
- Railroad Soils by Soil Washing. Proc. Of
- International Symposium on Mine reclamation.,
- pp: 160-161.
- Chungnam University, 2011.Verification report of
- soil remediation., pp: 8-9, 24-27.
- Margesin R., 2000. Potentiol of cold-adapted
- microorganisms for bioremediation of oil-polluted
- Alpine soils. International Biodeterioration &
- Biodegradation, 46:pp 3-10.
- Tajik M., 2004. Assessment of geoenvironmental
- effect of petroleum pollution on coastal sediments
- of Bushehr province. Iran.M.Sc. Thesis, tarbiat
- modares university, Tehran-Iran(in Persian) 97p.
- Ouyang W., Liu H., Murygina V., Yongyong Y.,
- Xiu Z., Kalyuzhnyi S. ,2005. Comparison of bioaugmentation
- and composting for remediation of
- oily sludge : A field-scale study in China. Process
- Biochemistry, 40: pp 3763-3768Prince,R.C. et al.,
- Bioremediationof oil spill. Oil & Gas and
- Technology-Rev, 58 (4), pp 463-468.
- Katsivela E., Moore E. R. B., Maroukli D.,
- Strompl C., Pieper D., Kalogerakis N., 2005.
- Bacterial community dynamics during in-situ
- bioremediation of petroleum waste sludge in
- landfarming sites. Biodegradation, 16: pp 169-180.