بررسی خصوصیات فیزیولوژیکی و فعالیت آنتی‌اکسیدانی ریحان (Ocimum basilicum cv. Keshkeni luvelou)تحت سطوح مختلف متیلجاسمونات و سمیت سرب
محورهای موضوعی : ژنتیک
سمیه اسدی
1
(گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران)
محمد مقدم
2
(گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه فردوسی مشهدف مشهد، ایران.)
عبدالله قاسمی پیربلوطی
3
(مرکز تحقیقات گیاهان دارویی، دانشگاه آزاد اسلامی واحد شهرقدس، تهران، ایران)
امیر فتوت
4
(گروه علوم خاک، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران)
کلید واژه: پرولین, محتوای نسبی آب, رنگیزه‌های فتوسنتزی, تنظیم کننده رشد, نشت الکترولیت,
چکیده مقاله :
آزمایشی به منظور بررسی تاثیر متیل جاسمونات بر خصوصیات فیزیولوژیکی و فعالیت آنتی اکسیدانی گیاه ریحان تحت سمیت سرب بصورت فاکتوریل در قالب طرح کاملا ً تصادفی در سه تکرار در گلخانه تحقیقاتی دانشگاه فردوسی مشهد انجام شد. تیمارهای آزمایش شامل سه سطح نیترات سرب (صفر (شاهد)، 200 و 400 میلی گرم در کیلوگرم خاک) و محلول پاشی متیل جاسمونات در سه غلظت (صفر، 5/0 و 1 میلی‌مولار) بودند. صفات مورد ارزیابی شامل میزان کلروفیل a و b، کلروفیل کل، کارتنوئیدها، نشت الکترولیت، رطوبت نسبی، فعالیت آنتی‌اکسیدانی، کربوهیدرات های محلول، فنل کل و پرولین بود. نتایح حاصل از این مطالعه نشان داد که در گیاهان تیمار شده با سرب، میزان پرولین، محتوای نسبی آب، کلروفیلa ، فعالیت آنتی اکسیدانی، فنل کل در مقایسه با گیاهان شاهد کاهش معنی داری نشان داد و کاربرد متیلجاسمونات (5/0 میلی مولار) در شرایط تنش سرب، باعث افزایش این صفات گردید. علاوه بر این در شرایط تنش سرب، میزان نشت الکترولیت به میزان قابل توجهی افزایش یافت و محلول پاشی متیل جاسمونات سبب کاهش آن شد. بنابراین، استفاده از متیلجاسمونات برای حفظ عملکرد اقتصادی گیاهان تحت تنش قابل توجیه است. همچنین استفاده از غلظت‌های 5/0 و 1 میلی مولار آن در شرایط تنش عنصر سنگین سرب با تحریک ساخت آنتی اکسیدانت ها، منجر به کاهش و تعدیل اثرات این تنش می شود.
This study was performed in order to evaluate the effect of methyl jasmonate on some physiological characteristics and antioxidant activity of basil under lead toxicity, based on a factorial and completely randomized design with three replications at Ferdowsi University of Mashhad Research Greenhouse. The experimental treatments included Pb (NO3)2) at three levels of (0, 200, and 400 mg/kg soil) and foliar application of methyl jasmonate at three concentrations (0, 0.5, and 1 mM). Measured traits included chlorophyll a, b, and total, carotenoids, electrolyte leakage, relative water content, antioxidant activity, soluble carbohydrate, total phenol, and proline. Results showed that in the plants treated with lead, the amount of proline, relative water content, chlorophyll a, antioxidant activity and total phenol reduced in comparison with control plants and application of methyl jasmonate (0.5 mM) under lead stress increased these traits. Furthermore, electrolyte leakage increased significantly under lead stress, while it was decreased by spraying with methyl jasmonate. Therefore, using methyl jasmonate is justified for protecting economic performance of plants under stress. Also, application of 0.5 and 1 mM methyl jasmonate reduced and adjusted the effects of stress in the plants under lead stress through stimulating production of antioxidants.
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Abdala, G., Miersch, O., Kramell, R., Vigliocco, A., Agostini, E., Forchetti, G. and Alemano, S. (2003). Jasmonate and octadecanoid occurrence in tomato hairy roots. Endogenous level changes in response to NaCl. Plant Growth Regulation. 40: 21-27.
Al-Amier, H. and Craker, L. E. (2007). In-vitro selection for stress tolerant spearmint. Issues in New Crops and New Uses, pp. 306-310.
Aldoobie, N.F. and Beltagi, M.S. (2013). Physiological, biochemical and molecular responses of common bean (Phaseolus vulgaris L.) plants to heavy metals stress. African Journal of Biotechnology. 12(29): 4614-4622.
Amirjani, M., Abnosi, M.H., Mahdieh, M. and Ghareshekhlou, S. (2015). Study of the effect of lead on the activity of antioxidant enzymes, proline and total alkaloids of callus of Catharantus roseus. Cell and Tissue Journal. 6(1): 9-21. (In Persian).
Arabaci, D. and Bayram, E. (2004). The effect of nitrogen fertilization and different plant densities on some agronomic and technologic characteristic of Ocimum basilicum L. (Basil). Journal of Plant Biology Research. 3(4): 255-62.
Arora, A., Byrem, T.M., Nair, M. and Strasburg, G.M. (2000). Modulation of liposomal membrane fluidity by flavonoids and isoflavonoids. Archives of Biochemistry and Biophysics. 373:102–109.
Asadi Karam, A., Asrar, Z. and Keramat, B. (2013). Effect of methyl-jasmonate treatment on the content of phenolic compounds and PAL activity in Lepidium sativum under the toxicity of copper. Journal of Plant Process and Function. 2(6): 89-96. (In Persian).
Ayala-Zavala J.F., Wang, S.Y., Wang, C.Y. and Gonzalez-Aguilar, G.A. (2005). Methyl jasmonate in conjunction with ethanol treatment increases antioxidant capacity, volatile compounds and post-harvest life of straw berry fruit. European Food Research and Technology. 221: 731-8.
Babst, B.A., Ferrieri, R.A., Gray, D.W., Lerdau, M., Schyler, D.J., Schueller, M., Thorpe, M.R. and Orians, C.M. (2005). Jasmonic acid induces rapid changes in carbon transport and partitioning in populus. New Phytologist. 167: 63-72.
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Barrientos Carvacho, H., Pérez, C., Zúñiga, G. and Mahn, A. (2014). Effect of methyl jasmonate, sodium selenate and chitosan as exogenous elicitors on the phenolic compounds profile of broccoli sprouts. Journal of the Science Food and Agriculture. 63(1): 20-31.
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