بررسی اثر نانوذره اکسیدروی بر رشد، رنگیزه‌های فتوسنتزی و اسمولیت‌های محلول گیاه نوروزک (Salvia leiifolia Benth.) تحت شرایط تنش شوری
محورهای موضوعی : ژنتیک
مهدی آخوندی
1
,
مریم نیاکان
2
,
هما محمودزاده
3
,
مجید دشتی
4
1 - گروه زیست‌شناسی، دانشگاه آزاد اسلامی، واحد گرگان، گرگان، ایران
2 - گروه زیست‌شناسی، دانشگاه آزاد اسلامی، واحد گرگان، گرگان، ایران
3 - گروه زیست‌شناسی، دانشگاه آزاد اسلامی، واحد مشهد، مشهد، ایران
4 - مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی خراسان رضوی، سازمان تحقیقات،
آموزش و ترویج کشاورزی، مشهد، ایران
کلید واژه: پرولین, شاخص‌های رشد, قند محلول, : Salvia leriifolia Benth, تنش شوری, کلروفیل نانواکسیدروی,
چکیده مقاله :
تنش شوری یکی از اصلی‌ترین عوامل محدودکننده رشد و تولید گیاهان می‌باشد. عنصر روی ازجمله عناصر ضروری در رشد و فرایندهای متابولیسمی می‌باشد که مصرف آن سبب مقاومت گیاهان به تنش‌های محیطی می‌شود. از سوی دیگر کاربرد عناصر در شکل نانو به‌علت سهولت جذب عناصر امروزه مورد توجه قرارگرفته است. به‌منظور بررسی اثر محلول‌پاشی نانواکسیدروی در شرایط تنش شوری، آزمایشی در قالب طرح فاکتوریل بر پایه طرح کاملاً تصادفی در سه تکرار اجرا شد. عوامل آزمایشی شامل دو سطح نانواکسیدروی (2 و 4 میلی‌گرم بر لیتر) و 5 سطح شوری (صفر، 50، 100، 150 و 200 میلی‌مولار) نمک کلرید سدیم در کنار شاهد بود. نتایج حاصل از این تحقیق نشان داد که طول ساقه، وزن‌تر گیاه، تعداد برگ، سطح برگ و میزان رنگیزه‌های فتوسنتزی و همچنین میزان کربوهیدرات‌های محلول در سطو مختلف شوری کاهش معنی‌داری یافت. از سوی دیگر تیمار 4 میلی‌گرم در لیتر نانواکسیدروی با تأثیر مثبتی بر پارامترهایی رشد، شاخص پایداری کلروفیل، میزان کلروفیل و کاروتنوئیدها، پرولین و قندهای محلول برگ و ریشه داشت. همچنین بررسی اثر متقابل داده‌ها نشان داد که بیشترین میزان طول ریشه و وزن‌تر کل در سطح 50 میلی‌مولار کلرید سدیم و نیز میزان پرولین وکلروفیل مربوط به سطح 100 میلی‌مولار کلرید سدیم همراه با محلول‌پاشی نانواکسیدروی با غلظت 4 میلی‌گرم در لیتر بود. به‌طورکلی در این تحقیق مشخص شد مصرف نانواکسیدروی با غلظت 4 میلی‌گرم در لیتر توانست سبب بهبود رشد گیاه و اسمولیت‌های سازگار و در نتیجه بهبود پاسخ گیاه نوروزک به تنش شوری گردد.
Salinity stress is one of the main factors limiting the growth and production of plants. Zinc is one of the essential elements in the plant growth and metabolic processes whose application causes the plants to withstand environmental stresses. On the other hand, application of nano-material has been considered recently for the ease of uptake. In order to investigate the effect of foliar application of ZnO nanoparticles under salinity stress, a factorial experiment was conducted based on a completely randomized design with three replications. Treatments consisted of two levels of ZnO solution at concentrations of 2 and 4 mg.l-1 and NaCl salinity at five levels of 0 (control), 50, 100, 150, and 200 mM. Results showed that stem length, plant fresh weight, leaf number, leaf area, and photosynthetic pigments content, as well as soluble carbohydrate content were significantly decreased compared to control especially at 200 mM salinity level. On the other hand, foliar application of 4 mgr/l nano zinc oxide through positive effects on parameters such growth parameters, chlorophyll stability index, chlorophylls, carotenoids, proline and soluble sugars content of leaf and root. Also, analysis of the interaction of effects of the treatments showed that maximum root length and total fresh weight at 50 mM NaCl and maximum proline and chlorophyll at 100 mM NaCl were observed in spraying nano zinc oxide with a concentration of 4 mg/l. In general, the results of the study showed that the application of 4 mg/l nano-zinc oxide resulted in an increase in the growth and compatible osmolytes of Salvia leriifolia Benth., improving its response to salinity stress.
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Latef, A.A.H.A., Alhmad, M.F.A., and Abdelfattah, K.E. (2017). The possible roles of priming with ZnO nanoparticles in mitigation of salinity stress in lupine (Lupinus termis) plants. Journal of Plant Growth Regulation, 36(1):60-70.
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Alpaslan, M., Inal, A., Gunes, A., Cikili, Y. and Oscan, H. (1999). Effect of zinc treatment on the alleviation of sodium and chloride injuryin tomato (Lycopersicon esculentum L.) grown under salinity. Turkish Journal of Botany, 23:1-6.
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Bates, L.S. (1973). Rapid determination of free proline for water-stress studies. Plant Soil, 39: 205–207.
Ben Taarit, M., Msaada, K., Hosni, K. and Marzouk, B., (2012). Physiological changes and essential oil composition of clary sage (Salvia sclarea L.) resette leaves as affectedby salinity. Acta Physiologiae Plantarum, 33:153-162.
Betran, F.J., Beck, D., Banziger, M. and Edmeades, G.O. (2003). Secondary traits in parental inbreeds and hybrids under stress and nonstress environments in tropical maize. Field Crops Research, 83:51-65.
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Chaparzadeh, N., Najjar-Khodabakhsh, A., Pazhang, M. and Zarandi-Miandoab, L. (2015). Effect of salinity and ascorbic acid on growth, water and osmotic relations of Lepidium sativum. Iranian Journal of Plant Biology, 7(24):39-52.
Chaves, M.M., Flexas, J. and Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany, 103: 551-560.
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Croser, C., Renault, S., Franklin, J. and Zwiazek, J. (2001). The effect of salinity on the emergence and seedling growth of Picea morian, Piccea glausa and Pinus banksiana. Environmental Pollution, 115:6-16.
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Doganlar, Z.B., Demir, K., Basak, H. and Gul, I. (2010). Effects of salt stress on pigment and total soluble protein contents of the three different Tomato cultivars. African Agricultural Journal. 5 (15): 2056-2065.
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Ehdaie, B., Alloush, G.A. Madore, M.A. and Waines, J.G. (2006). Genotypic variation for stem reserves and mobilization in wheat: I. postanthesis changes in internode dry matter. Crop Science, 46: 735- 746.
Ershad Langroudi, M. and Sedaghathoor, S. (2012). Effect of different media and salinity levels on growth traits of Rosemary (Rosmarinus officmali: L.). American-Eurasian Journal of Agriculture and Environment Science, 12 (9): 1134-1142.
Fathi, A., Zahedi, M., Torabian, Sh. and Khoshgoftar, A. (2017). Response of Wheat genotypes to foliar spray of ZnO and Fe nanoparticles under salt stress. Journal of Plant Nutrition, 40(10):1376–1385.
Fazeli Kakhki, S. and Goldani, M. (2018). Effects of Zinc oxide nanoparticles (ZnO) on improving morpho-physiological, yield and its components of Soybean (Glycine max L.) var. Williams under salinity stress. Journal of Crop Ecophysiology (Agriculture Science), 12(46(2)):253-268.
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Abbasi, A. and Enayati, V. (2013). Decrease of cell defense mechanisms efficiency and oxidative stress accruing in lake of Mg condition. Iranian Journal of Dryland Agriculture, 1 (4): 41-52.
Latef, A.A.H.A., Alhmad, M.F.A., and Abdelfattah, K.E. (2017). The possible roles of priming with ZnO nanoparticles in mitigation of salinity stress in lupine (Lupinus termis) plants. Journal of Plant Growth Regulation, 36(1):60-70.
Abdel-Haliem A.E.F., Hegazy S.H., Hassan N.S. and Naguib D.M. (2017). Effect of silica ions and nano silica on rice plants under salinity stress. Ecological Engineering, 99: 282–289.
Aghaei Joubani, K., Taei, N., Kanani, M.R. and yazdani, M. (2015). Effect of salt stress on some physiological and biochemical parameters of two Salvia species. Journal of Plant Process and Function Iranin Society of Plant Physiology, 3 (9):85-96.
Aghighi Shahverdi, M., Omidi, H. and Tabatabaei, S. (2017). Effect of foliar application of selenium, boron and iron on root morphological and photosynthesis pigments of stevia (Stevia rebaudiana Bertoni) under salinity stress. Journal of Iranian Plant Ecophysiological Research, 33(6):1017-1033.
Alharby, H.F., Metwali, E.M.R., Fuller, M.P. and Aldhebiani, A.Y. (2016). Impact of application of zinc oxide nanoparticles on callus induction, plant regeneration, element content and antioxidant enzyme activity in tomato (Solanum lycopersicum Mill.) under salt stress. Archives of Biological Science, 68(4):723-735.
Alpaslan, M., Inal, A., Gunes, A., Cikili, Y. and Oscan, H. (1999). Effect of zinc treatment on the alleviation of sodium and chloride injuryin tomato (Lycopersicon esculentum L.) grown under salinity. Turkish Journal of Botany, 23:1-6.
Arvin, P. (2015). Effect of gibberellin on some morphological traits, photosynthetic pigments content and proline in savory (Satureja hortensis L.) under salinity stress conditions. Journal of Agricultural Research, 7(2): 90-104.
Askary, M., Maghsoudi Moud, A.A. and Saffari, V.R. (2013). Investigation of some physiological characteristics and grain yield of corn (Zea mays L.) hybrids under salinity stress. Journal of Crop Production and Processing Isfahan University of Technology. 3 (9):93-104.
Askary, M., Talebi, Seyed M., Amini, F., Balout, B. and Dousti, A. (2017). Effects of iron nanoparticles on Mentha piperita L. under salinity stress. Biologija. 63:65-75.
Babaei, K., Sharifi, RS., Pirzad, A. and Khalilzadeh, R. (2017). Effects of bio fertilizer and nano Zn-Fe oxide on physiological traits, antioxidant enzymes activity and yield of wheat (Triticum aestivum L.) under salinity stress. Journal of Plant Interactions.12:381–389.
Bates, L.S. (1973). Rapid determination of free proline for water-stress studies. Plant Soil, 39: 205–207.
Ben Taarit, M., Msaada, K., Hosni, K. and Marzouk, B., (2012). Physiological changes and essential oil composition of clary sage (Salvia sclarea L.) resette leaves as affectedby salinity. Acta Physiologiae Plantarum, 33:153-162.
Betran, F.J., Beck, D., Banziger, M. and Edmeades, G.O. (2003). Secondary traits in parental inbreeds and hybrids under stress and nonstress environments in tropical maize. Field Crops Research, 83:51-65.
Broadley, M.R., White, P.J, Hammond, J.P, Zelko, I. and Lux, A. (2007). Zinc in plants. New Phytologist, 173 (4): 677-702.
Chaparzadeh, N., Najjar-Khodabakhsh, A., Pazhang, M. and Zarandi-Miandoab, L. (2015). Effect of salinity and ascorbic acid on growth, water and osmotic relations of Lepidium sativum. Iranian Journal of Plant Biology, 7(24):39-52.
Chaves, M.M., Flexas, J. and Pinheiro, C. (2009). Photosynthesis under drought and salt stress: regulation mechanisms from whole plant to cell. Annals of Botany, 103: 551-560.
Chinnusamy, V., Jagendorf, A. and Zhu, J.K. (2005). Understanding and improving salt tolerance in plants. Crop Science, 45:437-448.
Croser, C., Renault, S., Franklin, J. and Zwiazek, J. (2001). The effect of salinity on the emergence and seedling growth of Picea morian, Piccea glausa and Pinus banksiana. Environmental Pollution, 115:6-16.
Dashti, M., Kafi, M., Astaraei, A. and Zabihi, H. (2018). Investigation of yield and yield components response of Salvia leriifolia Benth. To the biological and organic Fertilizers. Zeitschrift fur Arznei-und Gewurzpflanzen, 22:84-90.
Dashti, M., Kafi, M., Tavakoli, H. and Mirza, M. (2015)(b). Effect of water deficit on water relation, photosynthesis and osmolytes accumulation of salvia leriifolia Benth. Iranian Journal of Field Crops Research, 12(4):813-821.
Doganlar, Z.B., Demir, K., Basak, H. and Gul, I. (2010). Effects of salt stress on pigment and total soluble protein contents of the three different Tomato cultivars. African Agricultural Journal. 5 (15): 2056-2065.
Dubois, M. and Gilles, K.A. (1956). Colorimetric method for determination of sugar and related substances. Analytical Chemistry, 25(3): 350-354.
Ehdaie, B., Alloush, G.A. Madore, M.A. and Waines, J.G. (2006). Genotypic variation for stem reserves and mobilization in wheat: I. postanthesis changes in internode dry matter. Crop Science, 46: 735- 746.
Ershad Langroudi, M. and Sedaghathoor, S. (2012). Effect of different media and salinity levels on growth traits of Rosemary (Rosmarinus officmali: L.). American-Eurasian Journal of Agriculture and Environment Science, 12 (9): 1134-1142.
Fathi, A., Zahedi, M., Torabian, Sh. and Khoshgoftar, A. (2017). Response of Wheat genotypes to foliar spray of ZnO and Fe nanoparticles under salt stress. Journal of Plant Nutrition, 40(10):1376–1385.
Fazeli Kakhki, S. and Goldani, M. (2018). Effects of Zinc oxide nanoparticles (ZnO) on improving morpho-physiological, yield and its components of Soybean (Glycine max L.) var. Williams under salinity stress. Journal of Crop Ecophysiology (Agriculture Science), 12(46(2)):253-268.
Ghorbanli, M. and Niakan, M. (2005). The effect of drought stress on soluble sugar, total protein, proline, phenolic compound, chlorophyll content and nitrate reductase activity in Soybean (Glycine max L. CV. Gorgan3). Journal of Science (Kharazmi University). 5(1-2): 537-550.
Hendawy, S.F.K. and Khalid, A. (2005). Response of sage Salvia officinalis L. plants to zinc application under different salinity levels. Journal of Applied Sciences Rrsearch. 1: 147-155.
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