Arbuscular Mycorrhizal fungi and precursor feeding improve protein and trypthopan decarboxylase enzyme content in Cathaaranthus roseus L. Samaneh Rahmatzadeh1*, Jalil Khara1 and Seyed Kamal Kazemitabar2
Subject Areas : Phytochemistry
Keywords: Mycorrhiza, SDS-PAGE, Catharanthus roseus L, tryptophan,
Abstract :
To obtain a better understanding of the mycorrhizas as an elicitor, and precursor feeding effects on the protein content and TIAs biosynthesis in Catharanthus roseus L., foliar application of four concentrations of tryptophan (0, 150, 250 and 350 mg/l) on some growth parameters and chemical compositions of mycorrhizal plants were investigated. The results revealed that the total protein content in the shoots and roots was higher respectively in G. etunicatum and G. versiforme mycorrhizal plants treated by 350 mg/l tryptophan, and this enhancement was significant at 5% statistical level. The evaluation of SDS-PAGE obtained results showed that the application of mycorrhizas and tryptophan led to significant accumulation of a 50-55 kDa M.W protein in the shoots and roots which probably could be attributed to TDC enzyme. From this observation we could suggest that the treatment of both mycorrhizas and tryptophan may be resulted in high expression of TDC enzyme.