Changes of morphological and physiological traits in Acer velutinum Boiss seedlings under drought stress

Subject Areas : Plant Physiology

Mokarram  Ravanbakhsh ¹ (Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran)
Babak  Babakhani ² (Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon,Iran)
Mahmood  Ghasemnezhad ³ (Department of Horticultural Science, University of Guilan, Guilan, Iran)
fariba  Serpooshan ⁴ (Department of Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran)
Mohamad Hassan  Biglouie ⁵ (5Department of Water Engineering, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.)

Received: 2021-08-27 Accepted : 2021-12-05 Published : 2023-01-01

Keywords: Water deficit, biomass, Antioxidant enzymes,

Abstract :

Water is needed for numerous metabolic functions and its loss can modify all characters of plant life. Acer velutinum is a fast-growing species and significant diffuse-porous hardwood in afforestation and reforestation. However, the impact of water stress on A. velutinum has not yet been studied. In this study, the impacts of water deficit on growth, biomass, leaf area, pigment contents, relative water content, free proline, malondialdehyde, superoxide dismutase, and peroxidase activities were studied to describe the morpho-physiological mechanisms related with their drought resistance. One-year Seedlings were exposed to four drought treatments (100, 75, 50, and 25% of the field capacity). The Drought treatments had negative impacts on A. velutinum growth, biomass, and physiological characteristics, as exhibited by decreased plant height, basal diameter, biomass, relative water content, and pigments level, while Proline, MDA concentration, SOD and POD activities multiplied. Drought increased SLA and LAR at mild and moderate treatment, while decreased SLA and LAR at severe treatment, also the growth declining in aboveground biomass was more than the root under severe treatment, so the Root mass ratio (RMR) and Root: Shoot ratio (R/S) expanded. Our data demonstrate that A. velutinum seedlings have the capacity to adapt to water deficit and can develop some experimental advice for velvet maple afforestation and reforestation.

References:

Abid, M., S. Ali, L.K. Qi, R. Zahoor, Z.Tian, D. Jiang, J.L. Snider and T. Dai .2018. Physiological and biochemical changes during drought and recovery periods at tillering and jointing stages in wheat (Triticum aestivum L.). Scientific Reports, 8(1)1–15.

Bargali, K., A.Tewari. 2004. Growth and water relation parameters in drought-stressed Coriaria nepalensis seedlings. Journal of Arid Environments, 58(4), 505-512.

Barros, V., A. Melo, M. Santos, L. Nogueira, G. Frosi and M. G. Santos. 2020. Different resource-use strategies of invasive and native woody species from a seasonally dry tropical forest under drought stress and recovery. Plant Physiology and Biochemistry, 147: 181–190.

Barrs, H.D. and P.E. Weatherley. 1962. A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian journal of biological sciences, 15, 413–428.

Bates L. S. 1973. Rapid determination of free proline for water -stress studies. Plant and Soil, 207: 205–207.

Bradford, M.M. 1976. A rapid and sensitive method for quantification of microgram quantities of pro- tein utilizing the principle of protein-dye binding. Analytical Biochemistry 72: 248–254.

Chakhchar, A., S. Wahbi, M. Lamaoui, A. Ferradous, A. El Mousadik, S. Ibnsouda-Koraichi, A. Filali-Maltouf, E. Cherkaoui, Modafar and C. El Modafar. 2015. Physiological and biochemical traits of drought tolerance in Argania spinosa. Journal of Plant Interactions, 10: 252–261.

Díaz-lópez, L., V. Gimeno, I. Simón, V. Martínez, W.M. Rodríguez-ortega, F. García-sánchez. 2012. Jatropha curcas seedlings show a water conservation strategy under drought conditions based on decreasing leaf growth and stomatal conductance. Agricultural Water Management, 105: 48–56

Farhadi, M., M. Tigabu, A.G. Arian, M.M. Sharifani, A. Daneshvar, and P.C. Oden. 2013. Pre-sowing treatment for breaking dormancy in Acer velutinum Boiss. seed lots. Journal of Forestry Research, 24: 273–278.

Gao, S., Y. Wang, S. Yu, Y. Huang, H. Liu, W. Chenand  X. He. 2020. Effects of drought stress on growth, physiology and secondary metabolites of Two Adonis species in Northeast China. Scientia Horticulturae, 259, 108795.

Ge, Y., X. He, J. Wang, B. Jiang, R. Ye  and X. Lin. 2014. Physiological and biochemical responses of Phoebe bournei seedlings to water stress and recovery. Acta Physiologiae Plantarum, 36, 1241-1250.

Giannopolitis, C.N., S.K. Ries, and J.M.C. Gutteridge. 1977. Superoxide dismutases: I. Occurrence in higher plants. Plant physiology, 59: 309–314.

Guo, J., Y. Yang, G. Wang, L. Yang and X. Sun. 2010. Ecophysiological responses of Abies fabri seedlings to drought stress and nitrogen supply. Physiologia Plantarum, 139: 335–347.

Guo, X., W. Guo, Y. Luo, X. Tan, , N. Du and R. Wang. 2013. Morphological and biomass characteristic acclimation of trident maple (Acer buergerianum Miq.) in response to light and water stress. Acta Physiologiae Plantarum, 35:1149–1159.

Guo, X., Y. J.J. Luo, Z. W.W. Xu, M. Y.Y. Li and W.-H.H. Guo. 2019. Response strategies of Acer davidii to varying light regimes under different water conditions. Flora, 257:151423.

Guo, Y.Y., H.Y. Yu, M.M. Yang, D.S. Kong and Y.J. Zhang. 2018. Effect of Drought Stress on Lipid Peroxidation, Osmotic Adjustment and Antioxidant Ezyme Activity of Leaves and Roots of Lycium ruthenicum Murr. Seedling. Russian Journal of Plant Physiology, 65: 244–250.

Hu, H., H. Chen, T. Hu, J. Zhang. 2012. Adaptability comparison between the seedlings of Eucalyptus grandis and Alnus cremastogyne under the condition of continuous drought stress. Journal of Agricultural Science, 4(4), 75-86.

Irigoyen, J.J., D.W. Emerich, and Sanchez-Diaz, M. 1992. Water stress induce changes in concentration of proline and total soluble sugar in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum 84:55-60

Khaleghi, A., R. Naderi, C. Brunetti, B.E. Maserti, S.A. Salami and M. Babalar. 2019. Morphological, physiochemical and antioxidant responses of Maclura pomifera to drought stress. Scientific Reports, 9: 1–12.

Lei, Y., C. Yin and C. Li. 2006. Differences in some morphological, physiological, and biochemical responses to drought stress in two contrasting populations of Populus przewalskii. Physiologia Plantarum, 127: 182–191.

Li, H.H., G. Han, Q.J. Wang and J. Fan. 2008. Effects of brassinolide on the survival, growth and drought resistance of Robinia pseudoacacia seedlings under water-stress. New Forests, 35: 255–266.

Liu, B., J.Liang, G.Tang, X. Wang, F. Liu, and  Zhao, D. 2019. Drought stress affects on growth, water use efficiency, gas exchange and chlorophyll fluorescence of Juglans rootstocks. Scientia Horticulturae, 250, 230-235.

Lichtenthaler, H.K. 1987. Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes. Methods in Enzymology, 148: 350–382.

Medeiros, D.B., E.C. da Silva, R.J.M.C. Nogueira, M.M. Teixeira, and M.S. Buckeridge. 2013. Physiological limitations in two sugarcane varieties under water suppression and after recovering. Theoretical and Experimental Plant Physiology, 25: 213–222.

 Naghdi, R., A. Solgi, E.R. Labelle and E.K. Zenner. 2016. Influence of ground-based skidding on physical and chemical properties of forest soils and their effects on maple seedling growth. European Journal of Forest Research, 135: 949–962.

 Naji, H.R., M.F. Nia, M. Kiaei, H. Abdul-Hamid, M. Soltani and A. Faghihi. 2016. Effect of intensive planting density on tree growth, wood density and fiber properties of maple (Acer Velutinum Boiss.). IForest, 9:325–329.

Picchio, R., F. Tavankar, M. Nikooy, G. Pignatti, R. Venanzi and A. Lo Monaco. 2019. Morphology, growth and architecture response of beech (Fagus orientalis Lipsky) and maple tree (Acer velutinum Boiss.) seedlings to soil compaction stress caused by mechanized logging operations. Forests, 10:(9)771.

Plewa, M.J., S.R. Smith and E.D. Wagner. 1991. Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation Research, 247: 57–64.

Ramezani, E., M.R. Marvie Mohadjer, H.D. Knapp, H. Ahmadi and H. Joosten. 2008. The late-Holocene vegetation history of the Central Caspian (Hyrcanian) forests of northern Iran. Holocene, 18: 307–321.

Sarker, U., S. Oba. 2018. Catalase, superoxide dismutase and ascorbate-glutathione cycle enzymes confer drought tolerance of Amaranthus tricolor. Scientific Reports, 8: (1)1–12.

Tariq, A., K. Pan, , O.A. Olatunji, C. Graciano, Z. Li, F. Sun, L. Zhang, X. Wu, W. Chen, D. Song, D. Huang, T. Xue and A. Zhang. 2018. Phosphorous fertilization alleviates drought effects on Alnus cremastogyne by regulating its antioxidant and osmotic potential. Scientific Reports, 8: 5644.

Toscano, S., E. Farieri, A. Ferrante and D. Romano. 2016. Physiologic al and biochemical responses in two ornamental shrubs to drought stress. Frontiers in plant science, 7: 645.

Wang, S., D. Liang, C. Li, , Y. Hao, F. Ma and H. Shu. 2012. Influence of drought stress on the cellular ultrastructure and antioxidant system in leaves of drought-tolerant and drought-sensitive apple rootstocks. Plant Physiology and Biochemistry. 51: 81–89.

 Wu, F., W. Bao, F. Li and N. Wu. 2008. Effects of drought stress and N supply on the growth, biomass partitioning and water-use efficiency of Sophora davidii seedlings. Environmental and Experimental Botany, 63: 248–255.

Wu, J., J. Li, Y. Su, Q. He, J. Wang, Q. Qiu and J. Ma. 2017. A morphophysiological analysis of the effects of drought and shade on Catalpa bungei plantlets. Acta Physiologiae Plantarum, 39:(3), 80.

Wu, M., W.H. Zhang, C. Ma and J.Y. Zhou. 2013. Changes in morphological, physiological, and biochemical responses to different levels of drought stress in chinese cork oak (Quercus variabilis Bl.) seedlings. Russian Journal of Plant Physiology. 60: 681–692.

Yang, F., L. F. Miao. 2010. Adaptive responses to progressive drought stress in two poplar species originating from different altitudes. Silva Fennica, 44(1), 23-37.

Ying, Y.Q., L.L. Song, D.F. Jacobs, L. Mei, P. Liu, S.H. Jin and J.S. Wu. 2015. Physiological response to drought stress in Camptotheca acuminata seedlings from two provenances. Frontiers in Plant Science, 6: 1–8.

Zhang, Y., T. Yu, W. Ma, C. Tian, Z. Sha and J. Li. 2019. Morphological and physiological response of Acer catalpifolium Rehd. Seedlings to water and light stresses. Global Ecology and Conservation, 19: e00660