Evaluation of traits and protein changes of Fornax cultivar of canola under salt stress treatment

Subject Areas : Stress Physiology

Mohammad Reza  Naghavi ¹ (Department of Agriculture, Payame Noor University (PNU), PO BOX 19395-4697 Tehran, Iran)
Abolfazl  Tavassoli ² (Department of Agriculture, Payame Noor University (PNU), PO BOX 19395-4697 Tehran, Iran)
Marouf  Khalili ³ (Department of Agriculture, Payame Noor University (PNU), PO BOX 19395-4697 Tehran, Iran)
Fatemeh  Rastegaripour ⁴ (Department of Agriculture, Torbat Heydariyeh University, Torbat Heydariyeh, Iran)

Received: 2022-12-07 Accepted : 2023-01-23 Published : 2022-07-01

Keywords: Salinity, Greenhouse, Oil crop, Proteomic analysis,

Abstract :

Canola oilis avegetable oilderived from a variety ofrapeseed with lowererucic acid contents. To investigate the mechanism of response to salinity induced by sodium chloride in canola, Fornax cultivar was evaluated in greenhouse conditions. Results showed that salinity stress affected morphological and physiological traits so that leaf relative water content and photosynthesis-related traits decreased while proline and glycine betaine concentrations were higher than in control plants. In addition, under stress conditions, imbalances in ionic concentrations were caused by an increase in sodium ion concentration and a decrease in intracellular potassium ion, as well as a decrease in the potassium / sodium ratio in leaf cells. Proteomic analysis results also detected 7 protein spots with expression difference and significant induction factor (IF) between control plants and severe salinity stress. Each protein, identified by mass spectrometry, was related to antioxidant defense, involved in photosynthetic light reaction, Calvin cycle, and nitrogen assimilation, respectively.

References:

Allen, J. F. 2003. Cyclic, pseudocyclic and noncyclic photophosphorylation: new links in the chain. Trends Plant Science, 8:15-19.

Alonso, M., M. J. Rozados, J. A. Vega, P. Perez-Gorostiaga, P. Cuinas, M. T. Fonturbel, and C. Fernandes. 2002. Biochemical responses of Pinus Pinaster tree to fire-induced trunk girdling and crown scorch: secondary metabolites and pigments as needle chemical indicators. Journal of Chemical Ecology, 28:687-700.

Andersson, I., and A. Backlund. 2008. Structure and function of Rubisco. Plant Physiology and Biochemistry, 46:275-291.

Ashraf, M., and Mc T. Neilly. 2004. Salinity tolerance in some brassica oilseeds. Critical Reviews in Plant Sciences, 23:154-174.

Bassi, R., D. Sandona, and R, Croe. 1997. Novel aspects of chlorophyll a/b-binding proteins. Physiology Plant, 100:769-779.

Bates, L. S., R. D. Walderen, and I. D. Taere. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39:205-207.

Cakmak, I. 2005. The role of potassium in alleviating detrimental effects of abiotic stresses in plants. Journal of Plant Nutrition Soil Science, 168:521-530.

Chimenti, C. A., J. Pearson, and A. J. Hall. 2002. Osmotic adjustment in Maize: Genetic variation and association with water uptake. In G. O. Edmeades (Ed.), Developing Drought and Low N-Tolerant Maize. (pp. 200-203). CIMMYT, Mexico.

Damerval, C., D. De Vienne., M. Zivy, and H. Thiellement. 1986. Technical improvements in two-dimensional electrophoresis increase the level of genetic variation detected in wheat-seedling roteins. Electrophoresis, 7:52-54.

Dhindsa, R. S. 1991. Drought stress, enzymes of glutathione metabolism, oxidation injury, and protein synthesis in Tortula ruralis. Plant Physiology, 95:648-651.

Dixon, D.P., A. Lapthorn, and R. Edwards. 2002. Plant glutathione transferases. Genome Biology, 3:1-10.

Edwards, R., D. P. Dixon, and V. Walbot. 2000. Plant glutathione S-transferases: enzymes with multiple functions in sickness and in health. Trends Plant Science, 5:193-198.

Francois, B. B. 2007. Effect of salinity on germination and seedling growth of canola. PhD thesis. Agricultural Sciences at the University of Stellenbosch. p.73.

Grieve, C. M., and S. R. Grattan. 1983. Rapid assay for determination of water solublequaternary ammonium compounds. Plant Soil, 70:303-307.

Hashimoto, M., M. Toorchi., K. Matsushita., Y. Iwasaki, and S. Komatsu. 2009. Proteome analysis of rice root plasma membrane and detection of cold stress responsive proteins. Protein and Peptide Letters, 16:685-697.

He, T., and G. R. Cramer. 1993. Growth and ion accumulation of two rapid-cycling Brassica species in response to seawater salinity. Plant Soil, 153:19-31.

Heide, H., H. M. Kalisz, and H. Follmann. 2004. The oxygen evolving enhancer protein 1 (OEE) of photosystem II in green algae exhibits thioredoxin activity. Journal of Plant Physiology, 161:139-149.

Herbert, B. 1999. Advances in protein solubilisation for two-dimensional electrophoresis. Electrophoresis, 20(4-5):660-663.

Holmstrom, K. O., S. Somersalo., A. Manda., T. E. Palva, and B. Welin. 2000. Improved tolerance to salinity and low temperature in transgenic tobacco producing glycine betaine. Journal of Experimental Botany, 51:177-185.

Hortensteiner, S, and B. Krautler. 2011. Chlorophyll breakdown in higher plants. Biochimica et Biophysica Acta, 1807: 977-988.

Houshmand, S., A. Arzani, S. A. M. Maibody, and M. Feizi. 2005. Evaluation of salt-tolerant genotypes of durum wheat derived from in vitro and field experiments. Field Crops Research, 91:345-354.

Ifuku, K., S. Ishihara, R. Shimamoto, K. Ido, and F. Sato. 2008. Structure, function, and evolution of the PsbP protein family in higher plants. Photosynthesis Research, 98:427-437.

Jin, J., K. Li, J. Qin, L. Yan, S. Wang, G. Zhang, X. Wang, Y. Bi. 2021. The response mechanism to salt stress in Arabidopsis transgenic lines over-expressing of GmG6PD. Plant Physiology Biology, 162:74-85.

Joseph, B, and D. Jini. 2010. Proteomic analysis of salinity stress-responsive proteins in plants. Asian Journal of Plant Science, 9:307-313.

Kattab, H. 2007. Role of glutathione and polyadenylic acid on the oxidative defense systems of two different cultivars of canola seedlings grown under saline condition. Austarilian Journal of Basic Appllied Science, 1(3):323-334.

Kausar, R., M. Arshad, A. Shahzad, and S. Komatsu. 2013. Proteomics analysis of sensitive and tolerant barley genotypes under drought stress. Amino Acid, 44:345-359.

Khalid, A., H. U. R. Athar, Z. U. Zafar, A. Akram, K. Hussain, F. Manzour, and M. Ashraf. 2015. Photosynthetic capacity of canola (Brassica napus L.) plants as affected by glycinebetaine under salt stress. Journal of Applied Botany and Food Quality, 88:78-86.

Khan, M. A., M. U. Shirazi, M. A. Khan, S. M. Mujtaba, E. Islam, S. Mumtaz, and M. Y. Ashraf. 2009. Role of proline, K⁺/Na⁺ ratio and chlorophyll content in salt tolerance of wheat. Pakistanian Journal of Botany, 41(2):633-638.

Kocheva, K., P. Lambrev, G. Georgiev, V. Goltsev, and M. Karabaliev. 2004. Evaluatlon of chlorophyll fluorescence and membrane injury in the leaves of barley cultivars under osmotic stress. Bioelectrochemistry, 63:127-124.

Komatsu, S., and N. Tanaka. 2004. Rice proteome analysis: A step toward functional analysis of the rice genome. Proteomics, 4:938-949.

Kumara, S. G., A. M. Reddy, and C. Sudhakar. 2003. NaCl effects on proline metabolism in two high yielding genotypes of mulberry (Morus alba L.) with contrasting salt tolerance. Plant Science, 165:1245-1251.

Macdonald, F. D., and B. B. Buchanan. 1997. The reductive pentose phosphate pathway and its regulation. In D.T. Dennis, D.H. Turpin, D.D. Lefebvre and D.B. Layzell (Eds.), Plant Metabolism (2nd ed., pp. 299-313). Essex: Addison Wesley Longman.

Mahmood, S. S., and H. R. Athar. 2003. Intra specific variability in sesame (Sesamum indicum L.) for various quantitive and qualitive attributes under differential salt regimes. Journal of Science Research, 14(2):177-186.

Maxwell, K., and G. N. Johnson. 2000. Chlorophyll fluorescence-a practical guide. Journal of Experimental Botany, 51:659-668.

Morant-Manceau, A., E. Pradier, and G. Tremblin. 2004. Osmotic adjustment, gas exchanges and chlorophyll fluorescence of a hexaploid triticale and its parental species under salt stress. Journal of Plant Physiology, 161:25-33.

Munns, R., R. A. James, and A. Lauchli. 2006. Approaches to increasing the salt tolerance of wheat and other cereals. Journal of Experimental Botany, 57:1025-1043.

Munns, R., and M. Tester. 2008. Mechanisms of salinity tolerance. Annual Review Plant Biology, 59:651-681.

Pandey, M., and S. Penna. 2017. Time course of physiological, biochemical, and gene expression changes under short-term salt stress in Brassica juncea L. The Crop Journal, 5(3):219-230.

Pessarakli, M. M., P. V. Morgan, and J. J. Gilbert. 2005. Dry- Matter yield, protein synthesis, starch, and fiber content of barley and wheat plants under two irrigation regimes. Journal of Plant Nutrition, 28(7):1227-1241.

Pillai, M. A., Z. Lihuang, and T. Akiyama. 2002. Molecular cloning, characterization, expression and chromosomal location of OsGAPDH, a submergence responsive gene in rice (Oryza sativa L.). Theoretical and Applied Genetics, 105:34-42.

Pinheiro, C., M. M. Chaves, and C. P. Ricardo. 2001. Alterations in carbon and nitrogen metabolism induced by water deficit in the stems and leaves of (Lupinus albus L.).
Journal of Experimental Botany, 52:1063-1070.

Ruuska, S. A., T. J. Andrews, M. R. Badger, G. D. Price, and S. von Caemmerer. 2000. The role of chloroplast electron transport and metabolites in modulating Rubisco activity in tobacco: insights from transgenic plants with reduced amounts of cytochrome b/f complex or glyceraldehyde 3-phosphate dehydrogenase. Plant Physiology, 122:491-504.

Saadia, M., A. Jamil, N. A. Akram, and M. Ashraf. 2012. A study of proline metabolism in canola (Brassica napus L.) seedlings under salt stress. Molecules, 17:5803-5815.

Sairam, R. K., K. V. Rao, and G. C. Srivastava. 2002. Differential response of wheat genotypes to long term salinity stress in relation to oxidative stress, antioxidant activity and osmolyte concentration. Plant Science, 163:1037-1046.

Sairam, R. K., and A. Tyagi. 2004. Physiology and molecular biology of salinity stress tolerance in plants. Current Science, 86:407-422.

Samaj, J., and J. J. Thelen. 2007. Plant proteomics. In J. Samaj and J. J. Thelen (Eds.), Plant proteomics. (pp. 20-32). Institute of Plant Genetics and Biotechnology, Slovak Republic.

Santos, C., A. Pereira., S. Pereira, and J. Teixeira. 2004. Regulation of glutamine synthetase expression in sunflower cells exposed to salt and osmotic stress. Science Horticulture, 103:101-111.

Schreiber, U., W. Bilper, and C. Neubauer. 1994. Chlorophyll fluorescence as a non-intrusive indicator for rapid assessment of in vivo photosynthesis. In E. D. Schulze and M. Caldwell (Eds.), Ecophysiology of photosynthesis. (Ecological studies, Vol 100). (pp. 49-70). Springer, Berlin Heidelberg, New York.

Sobhanian, H., K. Aghaei, and S. Komatsu. 2011. Changes in the plant proteome resulting from salt stress: Toward the creation of salt-tolerant crops? Journal of Proteomics, 74:1323-1337.

Stéphanie, M., Z. Bernard, and D. Habash. 2009. The importance of cytosolic glutamine synthetase in nitrogen assimilation and recycling. New Phytologist, 182:608-620.

Summart, J., P. Thanonkeo, S. Panichajakul, P. Prathepha, and M. T. Mc Manus. 2010. Effect of salt sress on growth, inorganic ion and proline accumulation in Thai aromatic rice, Khao Dawk Mali 105, Callus Culture. African Journal of Biotechnology, 9(2):145-152.

Takahashi, S., and N. Murata. 2008. How do environmental stresses accelerate photo inhibition? Trends Plant Science, 13:178-182.

Tamoi, M., M, Nagaoka, Y. Yabuta, and S, Shigeoka. 2005. Carbon metabolism in the Calvin cycle. Plant Biotechnology, 22:355-360.

Vaidyanathan, H., P, Sivakumar, R. Chakrabarty, and G. Thomas. 2003. Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.) differential response in salt-tolerant and sensitive varieties. Plant Science, 165:1411-1418.

Valdez-Aguilar, L., A. C. M. Grieve, J. Poss, and D. A. Layfie. 2009. Salinity and alkaline pH in irrigation water affect marigold plants: II.Mineral Ion Relations. Hortscience, 44(6):1726-1735.

Verslues, P. E., Y. S. Kim, and J. K. Zhu. 2007. Alterrd ABA, Proline and hydrogen peroxide in an Arabidopsis glutamate: glyoxylate aminotrasferase mutant. Plant Molecular Biology, 64:205-217.

Yang, Y. J. 2008. Proteome comparative analysis of Cryptochromes of Arabidopsis. Hunan University, Changsha.

Ye, J., S. Wang, F. Zhang, D. Xie, and Y. Yao. 2013. Proteomic analysis of leaves of different wheat genotypes subjected to PEG₆₀₀₀ stress and rewatering. Plant Omics Journal, 6(4):286-294.

Yıldız, M., N. Akçalı, and H. Terzi. 2015. Proteomic and biochemical responses of canola (Brassica napus L.) exposed to salinity stress and exogenous lipoic acid. Journal of Plant Physiology, 179:90-99.