Identification and Screening of Homozygous and Heterozygous Almond Progenies from Self-Pollinated Touno Cultivar Using PCR

Subject Areas : Microbiology

P. Najafi ¹ , A. Imani ² , S.M. Miri ³ , M. Zinalabdini ⁴

1 - Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran
2 - Department of Horticultural, Seed and Plant Improvement Iinstitute (SPII), Karaj, Iran
3 - Department of Horticulture, Karaj Branch, Islamic Azad University, Karaj, Iran
4 - Department of Molecular Genomics, Agricultural Biotechnology Research Institute (ABRII), Karaj, Iran

Received: 2015-11-26 Accepted : 2015-11-26 Published : 2015-11-01

Keywords: Hybrid, Almond, Self-compatible, Self-pollination,

Abstract :

Self-incompatibility is one of the most important difficulties in almond production which reduce fruit set dramatically and makes orchard management difficult. Therefore, breeding almond to produce self-compatible genotypes is very important. In this research identification and screening of 86 almond progenies obtained from selfing Touno after the self-pollination by PCR reaction with specific primers of CEBASf and AS1. PCR results confirmed the situation of self-compatible hybrids. In addition, it indicated that, frequencies of Sf, and S1 was 100% and 50% in progenies respectively. Self-compatible hybrids had been identified that can be used in almond breeding programs particularly to development the monoculture of almond orchards. So to identify and screening homozygous self-compatibility almonds be capable of be another step towards creating monoculture of almond and use in breeding programs further.