Effect of Budding Method, Rootstock Age and Cut below Budding Union on Budding Success in Persian Walnut
Subject Areas : MicrobiologyF. Ghamari Hesabi 1 , Y. Sharafi 2 , S.J. Tabatabaei 3 , V. Grigurian 4
1 - Department of Horticulture, Shahed University, Tehran, Iran
2 - Department of Horticulture, Shahed University, Tehran, Iran
3 - Department of Horticulture, Shahed University, Tehran, Iran
4 - Department of Horticulture, Tabriz University, Tabriz, Iran
Keywords: Persian walnut, Budding, Callus formation, grafting,
Abstract :
Budding is a good method of propagation in the Persian walnut (Juglans regia L.)., However, success of budding is low due to higher root pressure as well as the presence of phenolic compounds in the xylem sap. This study was conducted to compare various budding methods and cut below budding union on two and three-year-old Persian walnut under field conditions. The experiment was conducted based on a completely randomized block design with three replications. The first factor of experiment was budding methods at three levels (T-budding, invert T-budding and patch budding). The second factor was rootstock age at two levels (two-year and three-year old rootstocks).The third factor was to make cuts in the rootstock below budding union at two levels (cut below budding and no cut below budding). The results showed that budding method and rootstock age have significant effect on early percentage of budding success. The highest percentage of budding success (100%) was observed in three-year-old rootstock with T and inverted T-budding as well as in two-year-old rootstock with patch budding and the least success were gained in inverted T-budding on two-year-old rootstocks (69%). The highest callus formation was observed in patch budding on two-year-old, which is not differed by cut below budding treatment. The least callus formation was observed in T-budding without cut on two-year old rootstock (38%). Budding methods have a significant effect on secondary success (survival) at 1%. Patch budding method with 69% survival compared to other methods had most secondary success. Generally, in this study, patch budding had more callus formation, primary and secondary success compared to other budding methods.
Ebrahimi A, Vahdati K (2006) Improved success of Persian walnut grafting under environmentally controlled conditions. International Journal of Fruit Science. 6(4), 3-12. [In
Persian].
FAO. 2013. FAOSTAT database results. http://faostat3.Fao.org.faostat.
Gandev S (2007) Budding and grafting of the walnut (Juglans regia L.) and their effectiveness in Bulgaria (Review). Bulgarian Journal of Agricultural Science. 13, 683-689.
Gandev S, Arnaudov V (2011) Propagation method of epicotyl grafting in walnut (Juglans regia L.) under production condition. Bulgarian Journal of Agricultural Science. 17, 173-176.
Hartmann HT, Kester DE, Davies FTJR, Geneve LR (2002) Plant Propagation: Principles and Practices. Seventh Edition. Regents / Prentice Hall International Editions, Englewood Cliffs, New Jersey, 880.
Jacobs DF, Woeste KE, Wilson BC, McKenna JR (2006) Stock quality of black walnut (Juglans nigra L.) seedlings as affected by-sib seed source and nursery sowing density.
Acta Horticulturae. 705, 374-380
Lantos A (1990) Bench grafting of walnut. Acta
Horticulturae. 284, 53-57.
Pinghai D, Rongting X (1993) Effect of phenols on the survival of walnut grafting. Acta
Horticulturae. 311, 134-140.
Rezaee R, Vahdati K (2008) introducing a simple and efficient procedure for top working Persian walnut trees. Journal of the American Pomological Scociety. 62, 21-26. [In Persian]
Rezaee R, Vahdati K, Grigoorian V, Lizade M (2008) Walnut grafting success and bleeding rate as affected by different grafting methods and seedling vigour. Journal of Horticultural Science and Biotechnology. 83, 94-99. [In Persian]
Vahdati K (2003) Nursery management and grafting of walnut. Khaniran Publication, Tehran, Iran. pp. 99. [In Persian].
Weber GC, Mac Daniels LH (1969) Propagation, p. 29. In: R.A. Jaynes (Eds). Handbook of North American Nut Trees. Northern Nut Growers Assn., Knoxville, Tenn.