Relationship of Lipid Factors in Blood Serum and Seminal Plasma of Afshari Rams

Subject Areas : Camel

A. Pirestani ¹ , E. Ziya Motalebipour ²

1 - Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran|Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran|Medicinal Plants Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2 - Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran|Medicinal Plants Research Center, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran|Department of Agriculture, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran

Received: 2022-09-13 Accepted : 2022-09-13 Published : 2022-09-01

Keywords: sperm, blood, Lipid, Seminal plasma, Afshari ram,

Abstract :

The aim of this study was to investigate the relationship of lipid factors of seminal plasma and blood serum in Afshari rams. Four Afshari rams were selected with a mean weight of 50 ± 5 kg and the mean age of 2.5 years. At the same time, blood and semen samples were collected to evaluate total cholesterol, triglycerides, high-density lipoprotein (HDL), and low-density lipoprotein (LDL), while beta-hydroxybutyric acid (BHBA) was measured only in blood samples. After adding semen to the extender, sperm motility, morphology and viability were evaluated by the CASA software, Papanicolaou and Eosin-Nigrosin staining, respectively. The sperm parameters of seasonal and non-seasonal breeding were assessed in pre-freezing and post-thawing. The results of this study showed that cholesterol and HDL levels of ram were significantly higher in seasonal breeding than non-seasonal breeding in both blood and semen samples (P<0.05). In non-seasonal breeding, LDL and triglyceride levels of blood and semen samples were significantly increased respectively (P<0.05). In seasonal breeding, the sperm parameters such as motility, morphology, and viability of rams were significantly higher in pre-freezing. In non-seasonal breeding, total motility was higher significant in post-thawing (P<0.05). Consequently, in the current study, there was a significant correlation between triglyceride of blood and triglyceride of seminal plasma. Also, there was a significant correlation between blood LDL and seminal LDL/HDL ratios for non-seasonal records. Blood LDL/HDL was also significantly correlated with the seminal cholesterol/HDL, which could improve the sperm quality parameters of Afshari ram in the season and non-seasonal breeding.

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