Microsatellite Analysis DNA for Controlled Breeding of Cervidae and Genetic Breeding of White Colored Cervus elaphus in Czech Republic

Subject Areas : Camel

M. Emst ¹ , L. Putnova ² , J. Pokoradi ³ , J. Matouskova ⁴ , R. Zidek ⁵ , L. Marsalkova ⁶ , J. Lamka ⁷

1 - Department of ForestProtection and Game Management FFWT, MendelUniversity, Brno, Czech Republic
2 - Department of Animal Morphology, Physiology and Genetics FA, MendelUniversity, Brno, Czech Republic
3 - Xcell SlovakiaBreeding Services, SlovakRepublic
4 - Department of ForestProtection and Game Management FFWT, MendelUniversity, Brno, Czech Republic
5 - Department of Hygiene and Food Safety, Faculty of Biotechnology and Food Science, SlovakUniversityof Agriculture, SlovakRepublic
6 - Department of Hygiene and Food Safety, Faculty of Biotechnology and Food Science, SlovakUniversityof Agriculture, SlovakRepublic
7 - Department of Pharmaceutical Chemistry and Drug Control, Faculty of Pharmacy in Hradec Králové, Charles University, Prague, CzechRepublic

Received: 2011-11-01 Accepted : 2011-11-30 Published : 2012-09-01

Keywords: Microsatellite, Breeding, white colored deer, <i>Cervus elaphus</i>,

Abstract :

Aterritorial isolation is adanger to the game population, causing deterioration called the inbreeding depression. The typical signs are anomalous body proportions, such as short lower jaw, shortened lifetime, weak physique and lower birth rate. This is caused by low number of game in the community. White colored deer is in the location of the CzechRepublicbred in separation since 18th century. For their preservation, it was necessary to refresh the population by 'wild colored' Cervus elaphus. By this way, it was reduced the inbreeding depression and population was saved. Simultaneously white colored, beige colored, red colored, combined colored and spotted individuals started to appear. For further breeding development (retention of good health condition, fertility, inbreeding reduction and increase of white colored deer population), practical exploitation of molecular genetics methods were made by deer identification and verification of family relationships. Proven results were used for compilation of breeding groups with target of gradual reduction in: inbreeding, negative consequences of inbreeding depression and increase of white deer population (the origin from Kashmiror Persia). These breeding mechanisms are considered as breeding with controlled reproduction. This project is researching genetic variances within separated white deer population in game-preserve Žleby from 2004 to 2010. The idiotypes have been determined for thirteen microsatellite frequencies (BM888, OarFCB5, RM188, RT1, RT13, T26, T156, T193)and the genetic diversity, heterozygote contribution, polymorphic information content (PIC) and inbreeding factor (f) have been assessed during the project elaboration. Software Power Marker V 3.25 was used for elaboration of obtained results. The project results demonstrated the exploitation of molecular genetic methods for controlled game reproduction.

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