The Influence of Drinking Water Containing Lemon Juice and Thyme Supplemented Diet on Performance and some Blood Parameters of Broilers under Heat Stress

Subject Areas : Camel

ح.  بهبودی ¹ (Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran)
ا.  اسماعیلی‪پور ² (Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran)
ر.  میرمحمودی ³ (Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran)
م.  مظهری ⁴ (Department of Animal Science, Faculty of Agriculture, University of Jiroft, Jiroft, Iran)

Received: 2015-03-27 Accepted : 2015-04-30 Published : 2016-03-01

Keywords: chicken, heat stress, lymphocyte, Thyme, heterophil, lemon juice,

Abstract :

This study was conducted to find out the effects of drinking water containing lemon juice and thyme supplemented diet on growth performance, serum concentration of cholesterol and triglycerides, heterophils (H), lymphocytes (L), H/L ratio and enzyme activity of glutathione peroxidase (GPX) in broilers under heat stress condition. The experiment was carried out as a 2 × 3 factorial arrangement with 2 levels of lemon juice (0 and 2 mL/L of drinking water) and 3 levels of thyme (0, 0.5 and 1 % of diet). Each dietary treatment consisted of 4 replicate pens (9 birds/pen). The birds were kept at 34 ˚C ± 2 (9.00 to 17.00) from 25 to 42 d of age. Feed intake (FI), body weight gain (BWG), feed conversion ratio (FCR), H/L ratio and GPX activity and serum cholesterol concentration were not affected (P>0.05) by individual addition of lemon juice or thyme; whereas, combination effect of the two additives improved performance and reduced H/L ratio and serum triglycerides concentration (P≤0.05). Concurrent usage of 0.5 thyme and 2 mL/L of lemon juice increased FI and BWG by 229 and 165 g, respectively. Addition of 0.5 and 1% thyme to the diet and 2 mL/L lemon juice to drinking water reduced the serum triglycerides concentration by 9.8, 11.1 and 8.5 mg/dL, respectively (P≤0.05). It is concluded that the concurrent administration of 0.5% of thyme to diet and 2 mL/L lemon juice to drinking water provides a potential protective management strategy in preventing heat-stress-related reduction in the performance of broiler chickens.

References:

Amad A.A., Männer K., Wendler K.R, Neumann K. and Zentek J. (2011). Effects of a phytogenic feed additive on growth performance and ileal nutrient digestibility in broiler chickens. Poult. Sci. 90, 2811-2816.

Aviagen. (2007). Parent Stock Management Manual: Ross 308. Aviagen Ltd., Newbridge, UK.

Borges S., Da Silva A.F., Ariki J., Hooge D. and Cummings K. (2003). Dietary electrolyte balance for broiler chickens exposed to thermoneutral or heat stress environments. Poult. Sci. 82, 428-435.

Elagib H.A. and Omer H. (2012). Effect of dietary ascorbic acid on performance and immune response of heat stressed broiler chicks. Pakistan J. Nutr. 11, 216-220.

Ertas O.N., Güler T., Çiftçi M., Dalkilic B. and Simsek U.G. (2005). The effect of an essential oil mix derived from oregano, clove and anise on broiler performance. Int. Poult. Sci. 4, 879-884.

Gross W.B. and Siegel H.S. (1983). Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian. Dis. 27, 972-979.

Gursu M.F., Onderci M., Gulcu F. and Sahin K. (2004). Effects of vitamin C and folic acid supplementation on serum paraoxonase activity and metabolites induced by heat stress in vivo. Nutr. Res. 24, 157-164.

Hashemipour H., Kermanshahi H., Golian A. and Veldkamp T. (2013). Effect of thymol and carvacrol feed supplementation on performance, antioxidant enzyme activities, fatty acid composition, digestive enzyme activities and immune response in broiler chickens. Poult. Sci. 92, 2059-2069.

Hippenstiel F., Abdel-Wareth A.A.A., Kehraus S. and Südekum K.H. (2011). Effects of selected herbs and essential oils and their active components on feed intake and performance of broilers: a review. Arch. Geflügelk. 75, 226-234.

Hosseini S.A., Meimandipour A., Alami F., Mahdavi A. and Mohiti-Asli M. (2013). Effects of ground thyme and probiotic supplements in diets on broiler performance, blood biochemistry and immunological response. Italian J. Anim. Sci. 12, 116-120.

Kadam A.S., Lonkar V.D., Patodkar V.R., Kolangath S.M. and Bhosale T.A. (2009). Comparative efficacy of supplementation of natural (citrous limon juice), herbal and synthetic vitamin C on the immune response of broiler chicken during summer stress. Asian J. Poult. Sci. 3, 57-62.

Kutlu H. and Forbes J. (1993). Changes in growth and blood parameters in heat-stressed broiler chicks in response to dietary ascorbic acid. Livest. Prod. Sci. 36, 335-350.

Lambert R.J.W., Skandamis P.N., Coote P.J. and Nychas G.J.E. (2001). A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J. Appl. Microbiol. 91, 453-462.

Lawrence B.M. (2005). Antimicrobial / Biological Activity of Essential Oils. Allured Publishing Corporation, Illinois, United States.

Lin H., Jiao H., Buyse J. and Decuypere E. (2006). Strategies for preventing heat stress in poultry. World’s Poult. Sci. J. 62, 71-86.

Mashaly M.M., Hendricks G.L., Kalama M.A., Gehad A.E., Abbas A.O. and Patterson P.H. (2004). Effect of heat stress on production parameters and immune responses of commercial laying hens. Poult. Sci. 83, 889-894.

McDowell L. (1989). Vitamins in Animal Nutrition Comparative Aspects to Human Nutrition. Academic Press, London, UK.

McKee J., Harrison P. and Riskowski G. (1997). Effects of supplemental ascorbic acid on the energy conversion of broiler chicks during heat stress and feed withdrawal. Poult. Sci. 76, 1278-1286.

Nedyalka V., Yanishlieva E.M., Marinovaa M.H., Gordon V. and Raneva G. (1999). Antioxidant activity and mechanism of action of thymol and carvacrol in two lipid systems. Food Chem. 64, 59-66.

Pisoschi A.M., Pop A., Negulescu G.P. and Pisoschi A. (2011). Determination of ascorbic acid content of some fruit juices and wine by voltammetry performed at pt and carbon paste electrodes. Molecules. 16, 1349-1365.

Radwan N.L., Hassan R.A., Qota E.M. and Fayek H.M. (2008). Effect of natural antioxidant on oxidative stability of eggs and productive and reproductive performance of laying hens. Int. J. Poult. Sci. 7, 134-150.

Sahin K., Sahin N. and Yaralıoglu S. (2002). Effects of vitamin C and vitamin E on lipid peroxidation, blood serum metabolites and mineral concentrations of laying hens reared at high ambient temperature. Biol. Trace Elem. Res. 85, 35-45.

SAS Institute. (2001). SAS^®/STAT Software, Release 8. SAS Institute, Inc., Cary, NC. USA.

Sengül T., Yurtseven S., Cetin M., Kocyigit A. and Sogut B. (2008). Effect of thyme (Thymus vulgaris) extracts on fattening performance, some blood parameters, oxidative stress and DNA damage in Japanese quails. J. Anim. Feed Sci. 17, 608-620.

Siegel H.S. (1995). Stress, strains and resistance. Br. Poult. Sci. 36, 3-22.

Tatli Seven P., SevenI., Yılmaz M. and Simsek G. (2008). The effects of Turkish propolis on growth and carcass characteristics in broilers under heat stress. Anim. Feed Sci. Technol. 146, 137-148.

Thanissery R., Kathariou S. and Smith D.P. (2014). Rosemary oil, clove oil, and a mix of thyme-orange essential oils inhibit Salmonella and Campylobacter in vitro. J. Appl. Poult. Res. 23, 221-227.

Toghyani M., Tohidi M., Gheisari A.A. and Tabeidian S.A. (2010). Performance, immunity, serum biochemical and hemato-logical parameters in broiler chicks fed dietary thyme as alternative for an antibiotic growth promoter. African J. Biotechnol. 9, 6819-6825.