Optimization of Ultrasound-Assisted Extraction Conditions for Pigment Compounds of the Brown Algae Sargassum angustifolium Using Response Surface Methodology

Subject Areas :

Niloofar  Aghajanpoor Sourkohi ¹ (Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran)
Aria  Babakhani ² (1Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Iran 2 Department of Marine Science, Caspian Sea Basin Research Center, University of Guilan, Rasht, Iran)

Received: 2023-05-12 Accepted : 2023-08-04 Published : 2023-09-20

Keywords: Pigment, Sargassum angustifolium, Ultrasound-Assisted Extraction, Brown algae, Optimization,

Abstract :

In this study, the extraction of S. angustifolium brown alga pigments was investigated by ultrasound to identify process variables and optimize the extraction conditions. Experimental design using central composite design (CCD) with four independent variables including the percentage of ethanol concentration (at three levels of 50, 75 and 100%), extraction time (at three levels of 10, 20 and 30 minutes), solid-liquid ratio (at three levels 1: 5, 1:10 and 1:15) and the power of ultrasound (at three levels 80, 240 and 400 watts) was performed for the responses of chlorophyll a, total chlorophyll, total carotenoids and fucoxanthin. The results showed that the highest amount of total fucoxanthin and carotenoids was obtained in 75% ethanol, duration 20 minutes, solid-liquid ratio 1:5 and ultrasonic power of 240 watts. In the mentioned optimal conditions, fucoxanthin and total carotenoids were 0.42 and 1.11 mg/g, respectively. The values obtained in the experiment have a logical relationship with the values predicted by the software, which indicates the appropriateness of the models used.