ارزیابی و مقایسه میزان فنل، فلاونوئید کل، رزوراترول و ظرفیت آنتی‌اکسیدانی در میوه گونه‌های دارویی (Vitis vinifera)، (vera Pistacia)، (Sambucus nigra) و (Ilex spinigera)

محورهای موضوعی : بیوشیمی

روح اله دستور ¹ , داود بخشی ² , علیرضا علی اکبر ³

1 - دانشجوی دکتری گروه باغبانی، گیاهان دارویی و معطر، پردیس دانشگاهی2، دانشگاه گیلان، رشت، ایران
2 - دانشیار گروه علوم باغبانی، دانشکده علوم کشاورزی، دانشگاه گیلان، رشت، ایران
3 - دانشیار گروه شیمی دانشکده علوم پایه، دانشگاه گیلان، رشت، ایران

تاریخ دریافت : 1395/12/06 تاریخ پذیرش : 1396/04/04 تاریخ انتشار : 1396/06/01

کلید واژه: انگور, آنتی‌اکسیدان, پسته, فلاونویید, آقطی سیاه, خاس, فنل و رزوراترول, کروماتوگرافی مایع,

چکیده مقاله :

رزوراترول یک پلی فنل با ظرفیت آنتی اکسیدانی بالا است که توانایی مهار یا به تاخیر انداختن طیف گسترده‌ای از بیماری‌ها را دارد. انگور قرمز، پسته، آقطی سیاه وخاس از منابع رزوراترول هستند که در کشور ما به وفور وجود دارند. به همین منظور، در سال 1395 نمونه‌های گیاهی از 5 منطقه مختلف کشور جمع آوری گردید و عصاره‌گیری نمونه‌ها با استفاده از روش خیساندن (ماسراسیون) در مرحله رسیدگی کامل میوه انجام گرفت. سپس میزان فنل، فلاونویید کل، ظرفیت آنتی اکسیدانی و رزوراترول در انگورسیاه رقم شاهانی (Vitis vinifera cv. shahani) و رقم شاهرودی (Vitis vinifera cv. shahrodi)، پسته (vera cv. ohadiPistacia)، آقطی سیاه (Sambucus nigra) و خاس (Ilex spinigera) به‌ترتیب با روش های فولین-سیوکالتو، کلریمتری آلومینیوم کلراید و ظرفیت مهار رادیکال های DPPH و کروماتوگرافی مایع با کارایی بالا (HPLC) اندازه گیری شد. نتایج نشان داد که بیشترین مقدار فنل در پوست میوه آقطی سیاه و بیشترین مقدار فلاونوئید در پوست انگور شانی مشاهده شد. همچنین، بیشترین ظرفیت آنتی اکسیدانی در پوست آقطی سیاه و خاس گزارش شد. انگور شانی نیز غنی ترین منبع رزوراترول بود (003/0±04/0 میلی گرم بر گرم وزن خشک)، اما آقطی سیاه و دانه پسته اوحدی هم دارای مقادیر قابل توجهی رزوراترول بودند. به طورکلی، منابع گیاهی مورد بررسی در این تحقیق، قابلیت کاربرد در صنایع دارویی برای استخراج آنتی‌اکسیدان های طبیعی را دارا هستند.

چکیده انگلیسی:

Resveratrol is one of the most polyphenol with antioxidant capacity which has ability to inhibit or retard a wide variety of disease. Vitis vinife L., Pistacia vera L., Sambucus nigra L. and Ilex spinigera Loes. , which are abundant resveratrol sources in Iran. Therefore the fruits of these plants in ripening stage were collected in 2015 from different regions of Iran  and were extracted by maceration method. The total phenol, flavonoid, resveratrol content and antioxidant capacity were measured by using Folin-Ciocalteu, aluminum chloride colorimetric assay, high performance liquid chromatography procedure (HPLC) and DPPH radical scavenging, respectively. The results were showed that  the highest of total phenol and antioxidant capacity were observed  in Sambucus  nigra  and Ilex spinigera, while the  Vitis vinifera had the richest source of resveratrol (0/04±0.003 mg/g DW) and flavonoid, respectively. So in conclusion all of these plants could be a good natural antioxidant  with the  high resveratrol content in pharmaceutical industry

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