همبستگی شیموتایپ‌های Ramalina farinacea با عوامل اکولوژیکی در ایران با استفاده از LC-ESI-MS/MS
محورهای موضوعی : فیتوشیمی
مریم شریفی
1
*
,
ایرج مهرگان
2
*
,
محمد سهرابی
3
,
کامبیز لاریجانی
4
,
هری سیپمن
5
1 - دانشگاه آزاد واحد علوم تحقیقات تهران
2 - گروه زیست شناسی- واحد علوم و تحقیقات
3 - گروه زیست فناوری صنعتی و محیط زیست، پژوهشکده زیست فناوری، سازمان پژوهش های علمی و صنعتی ایران، تهران، ایران
4 - گروه زیست شناسی، دانشکده علوم وفناوری‌های همگرا، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران
5 - باغ و موزه گیاه شناسی برلین- داهلم، آلمان
کلید واژه: ارتفاع, پروفیل شیمیایی, جهت شیب, شیموتایپ, کروماتوگرافی مایع-طیف‌سنجی جرمی, گونه Ramalina farinacea,
چکیده مقاله :
در مطالعه حاضر، خواص فیتوشیمیایی را مشخص کردیم که به طور خاص در پنج اکوتیپ متمایز Ramalina farinacea از ایران (شامل کردکوی، شاهرود، گرگان، فریم و گلوگاه) مرتبط بودند تا تنوع شیموتایپ های درون گونه‌ها تشخیص داده شوند. تفاوت‌های کیفی بین پروفیل‌های شیمیایی با استفاده از کروماتوگرافی مایع-طیف‌سنجی جرمی (LC-ESI-MS/MS) در چند مرحله بررسی گردید. بر اساس نتایج LC-ESI-MS/MS، مواد شیمیایی زیر شناسایی شدند: متابولیت‌های (1) Atranol، (2) Divaric acid، (3) Evernic acid، (4) Ramalinaic acid، (5) Squamatic acid، (6) Sekikaic acid، (7) 4’-0-Methyl norsekikaic acid، (8) Glomellonic acid، (9) Conprotocetraric acid، (10) Usnic acid، (11) Nephrosterinic acid، (12) Dihydroxyoctadecenoic acid، (13) Hypoconstictic acid و دو ماده نا‌شناخته. آنالیزهاي آماري با استفاده از نرم‌افزار SPSS به روش Ward بر پایه صفات کمی و کیفی صورت گرفت که دندروگرام با دو خوشه اصلی ایجاد شد و نزدیکی آرایه‌هاي فریم و گلوگاه باهم در یک زیر خوشه و در زیر خوشه دیگر گرگان، در خوشه‌ی دیگر هم نزدیکی شاهرود و کردکوی کاملاً مشهود است. همچنین ضرایب همبستگی صفات مورد مطالعه، همبستگی مثبت جهت شیب، ارتفاع، رطوبت، بیشینه و میانگین دما با شیموتایپ‌ها را نشان داده که میتواند تفاوت‌هایی که در پروفیل‌های شیمیایی اکوتیپ‌های گلسنگ مشاهده می‌شود را توجیه نماید.
This study, identified specific phytochemical properties associated with five distinct ecotypes of Ramalina farinacea from Iran (including Kordkooy, Shahrud, Gorgan, Farim, and Galugah) to determine intraspecific chemotype diversity. In several steps, qualitative differences in chemical profiles were investigated using liquid chromatography-tandem mass spectrometry (LC-ESI-MS/MS). Based on LC-ESI-MS/MS results, the following chemicals were identified: Metabolites (1) Atranol, (2) Divaric acid, (3) Evernic acid, (4) Ramalinaic acid, (5) Squamatic acid, (6) Sekikaic acid, (7) 4’-0-Methyl norsekikaic acid, (8) Glomellonic acid, (9) Conprotocetraric acid, (10) Usnic acid, (11) Nephrosterinic acid, (12) Dihydroxyoctadecenoic acid, (13) Hypoconstictic acid and Two unknown substances. Statistical analyses using Ward's method in SPSS were performed based on quantitative and qualitative traits. The dendrogram revealed two main clusters. The arrays of Farim and Galugah were closely grouped in one subcluster, while Gorgan formed another subcluster. In a separate cluster, Shahrud and Kordkooy showed proximity. Correlation coefficients between the studied traits showed a positive correlation of slope direction, altitude, humidity, maximum, and mean temperature with the chemotypes. This could explain the observed differences in the chemical profiles of the lichen ecotypes, these findings may confirm the observed variations in the chemical profiles of the lichen ecotypes.
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