اثر تمرینات استقامتی بر پیام رسانی سنتز اسیدهای صفراوی در موش های مبتلا به کبد چرب غیرالکلی

محورهای موضوعی : فصلنامه زیست شناسی جانوری

مهری قلی پور ¹ , نجمه رضائیان ² , محمد کریمی ³ , صادق چراغ بیرجندی ⁴

1 - گروه فیزیولوژی ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران
2 - گروه فیزیولوژی ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران
3 - گروه فیزیولوژی ورزش، دانشگاه صنعتی قم، قم، ایران
4 - گروه فیزیولوژی ورزشی، واحد بجنورد، دانشگاه آزاد اسلامی، بجنورد، ایران

تاریخ دریافت : 1401/10/25 تاریخ پذیرش : 1401/12/05 تاریخ انتشار : 1402/03/01

کلید واژه: کبد چرب غیرالکلی, اسیدهای صفراوی, پیام رسانی, فعالیت ورزشی استقامتی,

چکیده مقاله :

بیماری کبد چرب غیرالکلی (NAFLD) شایع ترین اختلال عملکرد کبدی و یکی از علل مهم بروز مرگ و میر ناشی از بیماری های مزمن کبدی است. هدف از مطالعه حاضر بررسی اثر هشت هفته تمرینات استقامتی بر اسیدهای صفراوی (BA) کبدی، بیان نسبی گیرنده ایکس فارنزوئید (FXR)، عامل رشد فیبروبلاست 15 (FGF15)، گیرنده 4 عامل رشد فیبروبلاست (FGFR4) و پروتئین بتا کلوتو (KLB) در موش‌های مبتلا به کبد چرب غیرالکلی بود. در این مطالعه تجربی تعداد 16 سر موش صحرایی نر نژاد ویستار با وزن تقریبی 120-160 گرم، پس از القای شرایط NAFLD، با شش هفته رژیم غذایی پرچرب به طور تصادفی در دو گروه مساوی (8 سر در هر گروه) شامل گروه‌های؛ کنترل (C) و تمرین استقامتی (E) تقسیم شدند. گروه تمرین E در یک پروتکل تمرین فزاینده دویدن روی تردمیل با شدت 65 درصد حداکثر اکسیژن مصرفی و تواتر 5 روز متوالی در هفته شرکت کردند. 48 ساعت پس از آخرین جلسه تمرین، آزمودنی‌ها کشته شده و نمونه گیری بافت کبد و نمونه خون برای ارزیابی متغیرهای تحقیق انجام گردید. تجزیه و تحلیل داده ها با استفاده از آزمون t مستقل انجام شد. یافته ها نشان داد پروتکل تمرین E در مقایسه با گروه کنترل، منجر به کاهش معنادار در مقادیر کبدی BA شد (0001/0= p). همچنین افزایش معنادار در بیان نسبی FXR، FGF15، FGFR4 و مقادیر پروتئین KLB مشاهده شد (0001/0 = p). در مجموع به نظر می رسد فعالیت ورزشی استقامتی می توانند اثرات مثبتی بر متغیرهای درگیر در پیام رسانی سنتز اسیدهای صفراوی کبدی داشته باشد.

چکیده انگلیسی:

Non-alcoholic fatty liver disease (NAFLD) is the most common liver dysfunction and one of the most important causes of death from chronic liver diseases. The purpose of this study is to investigate the effect of eight weeks of endurance training compared to resistance training on liver bile acids (BA), relative expression of farnesoid X receptor (FXR), fibroblast growth factor 15 (FGF15), fibroblast growth factor receptor 4 (FGFR4) and beta protein Klotho (KLB) in rats with non-alcoholic fatty liver disease. In this experimental study, 16 Wistar rats with an approximate weight of 120-160 grams, after induction of NAFLD conditions, with six weeks of high-fat diet were randomly divided into two equal groups (8 rats in each group), including; Control (C), endurance training (E). group E participated in an incremental treadmill exercise protocol with an intensity of 65% of maximal oxygen consumption and a frequency of 5 sessions per week. 48 hours after the last training session, the subjects were sacrificed and liver tissue and blood samples were taken to evaluate the research variables. Data were analyzed by using independent t-test. The findings showed that E training protocols led to a significant decrease in liver BA values (p = 0.0001). Also, in the E training group, a significant increase in the relative expression of FXR, FGF15, FGFR4 and KLB protein levels  was observed (p = 0.0001). In conclusion, it seems that endurance exercise can have positive effects on the variables involved in the signaling of bile acid synthesis. .

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