مقایسه تأثیر ویتامین E و بره موم (پروپولیس) بر اینترلوکین-5 و بیان ژن STAT1 در موش های ویستار مبتلا به کبد چرب غیرالکلی

محورهای موضوعی : فصلنامه زیست شناسی جانوری

سعیده بارفروش ¹ , مریم بنانج ^{2 *} , رامین حاجی خانی ³ , هنگامه علی بیک ⁴

1 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
2 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران
4 - گروه زیست شناسی، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران

تاریخ دریافت : 1400/12/11 تاریخ پذیرش : 1401/07/23 تاریخ انتشار : 1401/12/01

کلید واژه: ویتامینE, بره موم, کبد چرب غیرالکلی, ژن STAT1, اینترلوکین-5,

چکیده مقاله :

بیماری کبد چرب غیرالکلی (NAFLD) یک نگرانی فزاینده برای سلامتی در سراسر جهان است. متأسفانه دانشمندان موفق به یافتن روش درمانی مناسب برای این بیماری نشده اند و نیاز به تحقیقات بیشتری دارد. مطالعات نشان داده اند که درمان با آنتی اکسیدان ها در این بیماری مؤثر است. در این مطالعه، اثر دو آنتی اکسیدان مختلف بر NAFLD القا شده به رت های ویستار نر ارزیابی شد. این مطالعه بر روی 32 سر رت نر ویستار با وزن 200 گرم انجام شد که بصورت تصادفی به چهار گروه هشت تایی تقسیم شدند. موش ها توسط امولوسیون پرچرب به میزان 2 میلی گرم بر کیلوگرم به مدت 40 روز با گاواژ تغذیه شدند. پس از ابتلا به کبدچرب، میزان اینترلوکین-5 (IL-5) سرمی اندازه گیری شد و همچنین با استفاده از Real-time PCR، میزان بیان ژن STAT1 تعیین گردید. یافته ها نشان داد که میزان IL-5سرمی در گروه بیمار بصورت معناداری نسبت به گروه کنترل افزایش داشت و تیمار گروه ها با ویتامین E و بره موم میزان اینترلوکین-5 را بصورت معناداری نسبت به گروه بیمار کاهش داد (05/0 > p). بعلاوه، بیشترین کاهش مربوط به گروه بره موم بود (05/0 > p). از طرفی دیگر، میزان بیان ژن STAT1 نیز در گروه مبتلا به کبدچرب بصورت معناداری نسبت به گروه کنترل افزایش دارد (05/0 > p)؛ درحالیکه، پس از تیمار با آنتی اکسیدان ها بیان این ژن کاهش یافت (05/0 > p). براساس نتایج بدست آمده، استفاده از بره موم و همچنین ویتامین E به عنوان آنتی اکسیدان های طبیعی با کاهش اینترلوکین التهابی و همچنین کاهش بیان ژن موثر در شکل گیری NAFLD، نقش درمانی امیدوارکننده ای در کبد چرب غیرالکلی دارند.

چکیده انگلیسی:

Nonalcoholic fatty liver disease (NAFLD) is a growing health concern worldwide. Unfortunately, scientists have not succeeded in finding a suitable treatment method for this disease, and more research is needed. Studies have shown that treatment with antioxidants is effective in this disease. In this study, the effect of two different antioxidants on NAFLD induced in male Wistar rats was evaluated. This study was conducted on 32 male Wistar rats weighing 200 grams, which were randomly divided into four groups of eight. Mice were fed by gavage with high-fat emulsion at the rate of 2 mg/kg for 40 days. After suffering from fatty liver, the level of interleukin-5 (IL-5) in the serum was measured and also the expression level of STAT1 gene was determined using real-time PCR. The results showed that the level of serum IL-5 in the patient group significantly increased compared to the control group, and the treatment groups with vitamin E and propolis, significantly decreased the level of interleukin-5 compared to the patient group (p < 0.05). In addition, the greatest decrease was related to the propolis group (p < 0.05). On the other hand, the level of STAT1 gene expression in the fatty liver group is significantly increased compared to the control group (p < 0.05); Whereas, after treatment with antioxidants, the expression of this gene decreased (p < 0.05). Based on the obtained results, the use of propolis and also vitamin E as natural antioxidants by reducing the inflammatory interleukin and also reducing the expression of genes effective in the formation of NAFLD, have a promising therapeutic role in non-alcoholic fatty liver disease.

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