اثر شش هفته تمرین هوازی و عصاره دارچین بر بیان ژن لپتین در بافت کبد موش های نر تغذیه شده با رژیم غذایی پرچرب

محورهای موضوعی : فصلنامه زیست شناسی جانوری

رقیه حسینی ¹ , طاهره باقرپور ² , نعمت اله نعمتی ³

1 - گروه تربیت بدنی و علوم ورزشی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
2 - گروه تربیت بدنی و علوم ورزشی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران
3 - گروه تربیت بدنی و علوم ورزشی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران

تاریخ دریافت : 1399/08/10 تاریخ پذیرش : 1399/10/09 تاریخ انتشار : 1401/06/01

کلید واژه: چاقی, کبد, عصاره دارچین, لپتین, ورزش هوازی,

چکیده مقاله :

دو عامل سطح لپتین سرم به واسطه تاثیر بر اشتها و توانایی کاهش انرژی دریافتی و میزان فعالیت بدنی به واسطه توانایی افزایش انرژی مصرفی، نقش بسیار مهم و تعیین کننده‌ای در کنترل وزن و توده چربی بدن دارند. مصرف برخی ترکیبات گیاهی ضد چاقی نظیر دارچین نیز می تواند در کاهش توده چربی بدن و کنترل چاقی موثر و مفید باشد. پژوهش تجریی حاضر با هدف تعیین اثرات اجرای شش هفته تمرین هوازی (پنج روز در هفته)، مصرف عصاره دارچین (200 میلی گرم به ازای هر گیلوگرم از وزن بدن) و غذای پرچرب (150 میلی لیتر امولسیون غذای پرچرب مازاد بر رژیم غذایی روزانه) بر بیان ژن لپتین در بافت کبد موش های نر تغذیه شده با رژیم غذایی پرچرب در قالب یک طرح پژوهشی چند گروهی با گروه کنترل انجام شد. برای بررسی میزان بیان ژن یا mRNA پروتئین‌های مورد نظر از روش Real Time-PCR استفاده شد. اثرات رژیم غذایی پرچرب, ترکیب رژیم غذایی پرچرب و تمرین هوازی، ترکیب رژیم غذایی پرچرب و دارچین و ترکیب رژیم غذایی پرچرب و تمرین هوازی و دارچین بر متغیر لپتین در بافت کبد چشمگیر و معنی دار بود, به طوری که مصرف غذای پرچرب باعث تغییرات نامطلوب بیان ژن شده ولی اجرای شش هفته تمرین و مصرف عصاره دارچین باعث تعدیل اثرات نامطلوب رژیم غذایی پرچرب شده و تغییرات مطلوبی را در بیان ژن و وزن بدن و وزن بافت کبد ایجاد کرده اند. به علاوه ترکیب اجرای شش هفته تمرین و عصاره دارچین به مراتب اثرات مطلوب تری را در تعدیل اثرات نامطلوب رژیم غذایی بر بیان این ژن داشته است. بنابراین توصیه می شود که همراه با اجرای تمرینات ورزشی هوازی از عصاره دارچین نیز استفاده شود.

چکیده انگلیسی:

Given their effect on appetite and the ability to reduce energy intake and physical activity, serum leptin levels play a key and decisive role in controlling body weight and fat mass. Consumption of some anti-obesity plant compounds such as cinnamon can also be useful in reducing body fat mass and controlling obesity. This experimental study was aimed at determining the effects of six weeks of aerobic exercise (five days a week), consumption of cinnamon extract (200 mg per kg of body weight) and high-fat food (150 ml of high-fat food emulsion in excess of daily diet). Expression of leptin gene in the liver tissue of male rats fed a high-fat diet was performed in a multi-group research design with a control group. Real Time-PCR was used to evaluate the expression of the gene or mRNA of the proteins. Takara Viragen’s kit was employed to prepare PCR master mix for cDNA. The effects of high-fat diet, combination of high-fat diet and aerobic exercise, combination of high-fat diet and cinnamon and combination of high-fat diet, aerobic exercise, and cinnamon on leptin variable in liver tissue were considerable and significant so that high-fat diet caused adverse changes in the expression of this gene. Nevertheless, six weeks of exercise and consumption of cinnamon extract moderated the adverse effects of a high-fat diet and caused favorable changes in the expression of this gene and body weight and liver tissue weight. In addition, the combination of six weeks of exercise and cinnamon extract had far more favorable effects in modulating the adverse effects of diet on the expression of this gene. Hence, it is recommended to use cinnamon extract along with aerobic exercise. .

منابع و مأخذ:

1. Badalzadeh R., Shaghaghi, M., Mohammadi M., Dehghan, G., Mohammadi Z. 2014. The effect of cinnamon extract and long-term aerobic training on heart function, biochemical alterations and lipid profile following exhaustive exercise in male rats. Coronary Artery Disease; 2: 8. [In Persion]


2. Bouassida A., Chamari K., Zaouali M., Feki Y., Zbidi A. 2010. Review on leptin and adiponectin responses and adaptations to acute and chronic exercise. British Journal of Sports Medicine, 44(9): 620-630.


3. Bouassida A., Zalleg D., Bouassida S. 2006. Leptin, Its Implication in Physical Exercise and Training. Journal of Sports Science and Medicine, 5: 172-181.


4. Bouassida A., Zalleg D., Zaouali M., Gharbi N., Feki Y., Tabka, Z. 2004. Effects of Exercise supra-maximal surles concentrations de la leptine plasmatique. Sciences and Sports, 19: 136-138.


5. Dishman, R.K., Washburn, R.A. and Heath, G.W. 2004. Physical Activity Epidemiology. Human Kinetics, Champaign.


6. Eftekhari E., Zafari, A., Gholami M., 2016.Physical activity, lipid profiles and leptin, The Journal of sports medicine and physical fitness, 56(4): 465-469.


7. Essig D.A.. Alderson N.L.. Ferguson M.A.. Bartoli, W.p.. Durstine J.L. 2000. Delayed Effects of Exercise on the Plasma Leptin Concentration. Metabolism, 49: 359-399.


8. Fatouros, I.G., Tournis, S., Leontsini, D., Jamurtas, A.Z., Sxina, M., Thomakos, P., Manousaki M., Douroudos I., Taxildaris K., Mitrakou A. 2005. Leptin and adiponectin responses in overweight inactive elderly following resistance training and detraining are intensity related. The Journal of Clinical Endocrinology and Metabolism, 90: 5970-5977.


9. Fisher J.S.. Van pelt R.E.. Zinder, O Landt M... Kohrt W.M.. 2001. Acute exercise effect on postabsorptive serum leptin. Journal of Applied Physiology, 91: 680-686.


10. Gippini A., Mato A., Peino R., Lage, M., Dieguez C., Casanueva F.F. 1999. Effect of resistance exercise (body building) training on serum leptin levels in young men. Implications for relationship betwtween body mass index and serum leptin. Journal of Endocrinological Investigation, 22: 824-828.


11. Gomez-Merino, D., Chennaui, M., Drogou C., Bonneau D., Guezennee, C.Y. 2002. Decrease in serum leptin after prolonged physical activity in men. Medicine and Science in Sports and Exercise, 34: 1594-1599.


12. Hasani-Ranjbar S., Nayebi, N., Larijani, B., Abdollahi, M. 2009. A systematic review of the efficacy and safety herbal medicine used in the treatment of obesity, World Journal of Gastroenterology, 1525: 3073. [In Persion]


13. Hickey M.S., Houmard J.A..Considine, R.V., Tyndall, G.L., Midgette, J.B., Gvigan, K.E., Weidner, M.L., McCammon, M.R., Caro, J.F.1997. Gender-dependent effects of exercise training on serum leptin levels in humans. American Journal of Physiology, 272: E562-E566.


14. Houmard, J.A., Cox J.H., Mac-Lean, P.S., Barakat, H.A..2000. Effect of short-term exercise training on leptin and insulin action. Metabolism, 49: 858-861.


15. Ishii, T., Yamakita, T., Yamagami, K., Yamamoto. T., Miyamoto, M., Kawasaki, K., Hosoi, M., Yoshioka, k., Sato, T., Tanaka, S., Fujii, S., 2001. Effect of exercise training on serum leptin levels in type 2 diabetic patients. Metabolism, 50: 1136-1140.


16. Kanaley, J.A., Fenicchia, L.M., Miller, C.S. 2001. Restingleptinresponses to acute and chronic resistance training in type 2diabetic men and women,” International Journal of Obesity, 25(10): 1474-1480.


17. Karamouzis, I., Karamouzis, M., Vrabas, I.S., Christoulas, K., Kyriazis, N., Giannoulis, E., Mandroukas, K. 2002. The Effects of marathon swimming on serum leptin and plasma neuropeptide Y levels. Clinical Chemistry and Laboratory Medicine, 40: 132-136.


18. Kraemer, R.R., Johnson, L.G. Haltom, R. et al..1999 “Serum leptin concentrations in response to acute exercise in postmenopausal women with and without hormone replacementtherapy. Proceedings of the Society for Experimental Biology and Medicine, 221(3): 171-177.


19. Kraemer, R.R., Acevedo, E.O., Synovitz, L.B., Hebert, E.P., Gimpel, T., Castracane, V.D. 2001. Leptin and Steroid hormone response to exercise in adolescent female runners over a 7-week season. Eur Journal of Applied Physiology, 86: 85-91.


20. Kraemer, R.R., Chu, H., Castracane, V.D. 2002. Leptin and Exercise. Experimental Biology and Medicine, 227: 701-708.


21. Kraemer, R.R., Johnson, L.G., Halton, R.W., Hebert, E.P., Gimpel, T., Castracane, V.D..1999. Serum leptin concentrations in response to acute exercise in postmenopausal females with and without replacement therapy. Proceedings of the Society for Experimental Biology and Medicine, 221: 171-177.


22. Kraemer, R.R., Kraemer, G.R., Acevedo, E.O., Hebert, E.P., Temple, E., Bates, M., Etie, A., Haltom, R., Quinn, S. 1999b. Effects of aerobic exercise on serum leptin levels in obese women. European Journal of Applied Physiology and Occupational Physiology, 80: 154-158.


23. Kyriazis, G.A.2005.The Effects of a Single Exercise Bout on Plasma Leptin Concentration in Obese Males. A thesis proposal submitted for the degree of Master of Arts in the College of Education at the University of Central Florida, Orlando.


24. Landt, M., Lawson, G.M., Helgeson, J.M., Davila- Roman, V.G., Ladenson, J.H., Jaffe, A.S., Hickner, R.C. 1997. Prolonged Exercise decreases serum leptin concentrations. Metabolism, 46: 1109-1112.


25. Le Mura, Linda M., Duvillard, P.2004. Clinical Exercise physiology. Philadelphia: LWW.


26. Leal-Cerro, A., Garcia-Luna, P.P., Astorga, R., Parejo, J., Peino, R., Dieguez, C., Casanueva, F.F. 1998. Serum leptin levels in male marathon athletes before and after marathon. Journal of Clinical Endocrinology and Metabolism, 83: 2376-2379.


27. Mathew, S., Abraham, T.E. 2006. Studies on the antioxidant activities of cinnamon (Cinnamomum verum) bark extract, through various in vitro models. Food Chemistry, 94(4): 520-528.


28. Mollazadeh, H., Hosseinzadeh, H. 2016. Cinnamon effects on metabolic syndrome: a review based on its mechanisms. Iran Journal of Basic Medical Sciences, 19: 1258-1270.


29. Nasery, L., Zafari, A., Banaeifar, A. A..2015. The respose of leptin and lipid parameters related to an aerobic exercise among young athlete and non-athlete women. Indian Journal of Fundamental and Applied Life Sciences, 5(S3):788-798.


30. Nieman, D.C. 2003. Exercise Testing and Prescription. 5th Ed. New York: MHHE.


31. Nindl, B.C., Kraemer, W.J., Arciero, P.J., Samatallee, N., Leone, C.D., Mayo, M.F., Hafeman, D.L. 2002. Leptin Concentrations Experience a Delayed Reduction after Resistance Exercise in Men. Medicine and Science in Sports and Exercise, 34: 608-613.


32. Okazaki, T., Himeno, E., Nanri, H., Ogata, H., Ikeda, M. 1999. Effects of mild Aerobic Exercise and mild Hypocaloric Diet on plasma leptin in Sedentary Women. Clinical and Experimental Pharmacology and Physiology, 26: 415-420.


33. Olive, J.L., Miller, G.D. 2001. Differential Effects of Maximal-and moderate-intensity Runs on plasma leptin in healthy Trained Subjects. Nutrition, 17: 365-369.


34. Ossanloo, P., Najar, L., Zafari, A..2012. The Effects of Combined Training (Aerobic Dance, Step Exercise and Resistance Training) on Body Fat Percent and Lipid Profiles in Sedentary Females of Al-Zahra University. European Journal of Experimental Biology, 2(5): 1598-1602.


35. Roussel, A.M., Hininger, I., Benaraba, R., Ziegenfuss, T.N., Anderson, R.A. 2009. Antioxidant effects of a cinnamon extract in people with impaired fasting glucose that are overweight or obese. Journal of the American College of Nutrition, 28(1): 16-21.


36. Sartorius, T., Peter, A., Schulz, N., Drescher, A., Bergheim, I., Machann, J. 2014. Cinnamon extract improves insulin sensitivity in the brain and lowers liver fat in mouse models of obesity, PloS One, 9(3): e92358.


37. Shalaby, M.A., Saifan, H.A. 2014. some pharmacological effects of cinnamon and ginger herbs in obese diabetic rate. Journal of Intercultural Ethnopharmacology, 3(4): 144.


38. Thomas, T.R., Warner, S.O., Dellsperger, K.C., Hinton, P.S., Whaley-Connell, A.T., Rector, R.S., Liu, Y., Linden, MA., Chockalingam, A., Thyfault, J.P., Huyette, D.R., Wang, Z., Cox, R.H. 2010. Exercise and the metabolic syndrome with weight regain. Journal of Applied Physiology, 109(1): 3-10.


39. TOkura, T., Nakata, Y., Ohkawara K., Numao S., Katayama, Y., Matsuo, T., Tanaka, K. 2007. Effects of aerobic exercise on metabolic syndrome improvement in response to weight reduction. Obesity, 15(10): 2478-2484.


40. Torjman, M.C., Zafeiridis, A., paolone, A.M., Wilkerson, C., Considine, R.V., 1999. Serum leptin during recovery following maximal incremental and prolonged exercise. International Journal of Sports Medicine, 20: 444-450.


41. Unal, M., Unal, D.O., Baltaci, A.K., Mogulkoc, R. 2005. Investigation of Serum Leptin levels and VO2max Value in Trained Young Male Athletes and Healthy Males. Acta Physiology Hungary, 92: 173-179.


42. Unal, M., Unal, D.O., Baltaci, A.K., Mogulkoc, R., kaysrilioglu, A. 2005. Investigation of Serum Leptin Levels in professional Male Football plyaers and Healthy Sedentary Males. Neuro Endocrinology Letters, 26: 148-151.


43. Unal, M., Unal, D.O., Salman, F., Baltaci, A.K., Mogulkoc, R. 2004. The Relation Between Serum Leptin Levels and Max VO2 in Male Patients with Type I Diabetes and Healthy Sedentary Males. Endocrine Research, 30: 491-498.


44. Webber, J., 2003. Energy balance in obesity. Proceeding of Nutrition Society, 62(2): 539-543.


45. Weltman, A., pritzlaff, C.J., Wideman, L., Considine, R.V., Fryburg, D.A., Gutgesell, M.E., Hartman, M.L., Veldhuis, J.D.2000. Intensity of Acute Exercise does not Affect Serum Leptin Concentrations in Young Men. Medicine and Science in Sports and Exercise, 32: 1556-1561.


46. Whaley, M.H., Kaminski, L.A. 2001. Epidemiology of Physical Activity, Physical Fitness and Selected Chronic Diseases. Pp. 17-33. In ACSM's Resource Manual for Guidelines for Exercise Testing and Prescription. 4th Ed., Baltimore: Williams and Wilkins.


47. World Health Organization. 2000. Obesity: Preventing and Management the Global Epidemic. Geneva: WHO.


48. Zaccaria, M., Ermolao, A., Roi, G.S., Englaro, P., Tegon, G., Varnier, M. 2002. Leptin Production after Endurance Races Differing in Duration and Energy Expenditure. European Journal of Applied physiology, 87: 108-111.


49. Zafeiridis, A., Smilios, I., Considine, R.V., Tokmakidis, S.P. 2003. Serum Leptin Responses Following Acute Resistance Exercise Protocols. Journal of Applied Physiology, 94: 591-597.


50. Zoladz, J.A., Konturek, S.J., Duda, K., Majerczak, J., Sliwowski, Z., Grandys, M., Bielanski, W. 2005. Effect of Moderate Incremental Exercise, Performed in Fed and Fasted State on Cardiorespiratory Variables and Leptin and Ghrelin Concentrations in Young Healthy Men. Journal of Physiology and Pharmacology, 56: 63-85.