اثر هشت هفته تمرینات مقاومتی هوازی بر بیان ژن ABCG8 و پروفایل لیپیدی بیماران CABG

محورهای موضوعی : فصلنامه زیست شناسی جانوری

فریدا صادقی فاضل ¹ , امیر رشیدلمیر ² , رامبد خواجه ای ³ , اکبر صفی پور افشار ⁴

1 - گروه فیزیولوژی ورزشی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران
2 - گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی، دانشگاه فردوسی مشهد، مشهد، ایران
3 - گروه فیزیولوژی ورزشی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران
4 - گروه زیست‌شناسی، واحد نیشابور، دانشگاه آزاد اسلامی، نیشابور، ایران

تاریخ دریافت : 1399/05/05 تاریخ پذیرش : 1399/08/09 تاریخ انتشار : 1399/12/01

کلید واژه: پروفایل لیپیدی, بیماران CABG, تمرینات مقاومتی- هوازی, ژن ABCG8,

چکیده مقاله :

بیماری عروق کرونر قلب یکی از علل اصلی مرگ‌ومیر در جوامع امروز است. این بیماری با فعالیت ورزشی و بیان برخی ژن‌های درگیر در انتقال کلسترول خون در ارتباط است. پروتئین‌های جعبه ایی وابسته به آدنوزین تری فسفات 8 (ABCG8) نقش کلیدی در متابولیسم کلسترول و لیپوپروتئین با دانستیه بالا ایفا می‌کند. هدف از این پژوهش بررسی تأثیر هشت هفته تمرینات مقاومتی-هوازی بر بیان ژن ABCG8 و پروفایل لیپیدی در بیماران CABG بود. 30 مرد بیمار CABG به‌طور داوطلبانه انتخاب و به‌صورت تصادفی به دو گروه تجربی(15 نفر) و کنترل (15 نفر) تقسیم شدند. گروه تجربی به مدت هشت هفته تمرینات مقاومتی- هوازی را انجام دادند (3 جلسه در هفته) و گروه کنترل در این مدت بدون تمرین بودند. به‌منظور بررسی اندازه‌گیری‌ها، نمونه‌گیری خونی 48 ساعت قبل از شروع اولین جلسه تمرینی و همچنین 48 ساعت پس از آخرین جلسه تمرینی درحالی‌که همه آزمودنی‌ها ناشتا بودند به عمل آمد. برای تحلیل داده‌ها از آزمون آماری تی-استودنت مستقل و همبسته در سطح معنی‌داری 05/0 > p استفاده شد. نتایج پژوهش حاضر نشان داد گروه تمرینات مقاومتی-هوازی درنتیجه‌ی هشت هفته تمرین افزایش معنی‌داری را در بیان mRNA ژن ABCG8 و کاهش معنادار LDL-C و تری گلیسیرید و افزایش HDL-C در مقایسه با گروه کنترل داشته است. بر اساس یافته‌های تحقیق حاضر می‌توان چنین نتیجه‌گیری کرد که تمرینات مقاومتی- هوازی با افزایش بیان ژن ABCG8 برافزایش روند انتقال کلسترول تأثیر بالایی داشته باشد و درنتیجه احتمالاً سبب بهبود بیماران CABG و پیشگیری از بیماری‌های قلبی عروقی می‌گردد.

چکیده انگلیسی:

Coronary heart disease is one of the leading causes of death in today's societies. It is associated to the exercise activity and the expression of some genes involved in the blood cholesterol transmission. Adenosine triphosphate-dependent box protein 8 is a member of ABC transporter family which plays a key role in the metabolism of high-density lipoprotein cholesterol and cholesterol. This study aimed to evaluate the effect of eight weeks of aerobic resistance training on ABCG8 gene expression and lipid profile in CABG patients. 30 men with CABG patients voluntarily selected and randomly divided into the experimental (n = 15) and control (n = 15) groups. The experimental group performed the aerobic resistance training for eight weeks (3 sessions per week) and the control group did not. Blood samples were taken 48 h before the first training session and 48 h after the last training session, while all subjects were fasting. Independent and paired t-test used to analyze the data at a significant level of 0.05. The present study's results showed that the aerobic resistance training group showed a significant increase in the ABCG8 mRNA expression and a significant decrease in LDL-C, triglyceride, and HDL-C as compared to the control group. Due to the findings of present study, it can be concluded that aerobic resistance training by increasing ABCG8 gene expression has a great effect on increasing the cholesterol transfer process; thus, it may improve the CABG patients and prevent cardiovascular diseases.

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