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Manuscript ID : JCHR-2310-1861 (R1) Visit : 188 PDF XML

Article Type: Original Research

Molecular Identification of Lactic Acid Bacteria with Probiotic Potential from Local Curd

Melika Hassanzadeh Golijan 1 ( Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran )
Hossein Abbaspour 2 ( Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran )
Nazila Arbab Soleimani 3 ( Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran )

Submited date : 2023-09-30 Accepted date : 2023-12-04

Keywords: Lactic acid bacteria, Kashk, Probiotic potential,

Abstract :

Researchers believe that lactic acid bacteria isolated from native sources that have probiotic potential can be beneficial for improving health. The aim of this study was molecular identification of the best lactic acid with probiotic potential from curd (Kashk). 10 samples of traditional Kashk were gathered and isolation of lactic acid bacteria was done. Identification of lactic acid bacteria using common biochemical tests was performed. The antagonistic activity of isolated bacteria was investigated by the modified double-layer method. The anti-adhesion effect of isolated lactic acid bacteria was evaluated by the 96-well microtiter plate method. Molecular identification of the best probiotic lactic acid bacterium was done by ribotyping method and the phylogeny tree was drawn. From Kashk samples, 9 lactic acid bacteria were isolated. 7 isolates were able to grow at all pH levels tested, while isolates K4 and K8 did not grow at pH equivalent to gastric acid. In terms of bile salt tolerance, all isolates showed the ability to tolerate bile salts, and isolates K2, K3, and K7 had the highest tolerance. The results of the double-layer and anti-attachment method showed that isolate K2 was the most effective against the tested pathogens S. aureus, E. faecalis, E. coli, and P. aeruginosa. According to the phylogeny tree, the most effective probiotic lactic acid bacterium (K2) was known as Lactobacillus plantarum. In this research, it was found that Lactobacillus plantarum with probiotic potential can have antagonistic and anti-attachment effects against pathogenic bacteria and is effective for the treatment and prevention of bacterial diseases and ameliorate human health.

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