In vitro Cytotoxicity Effect of Lactobacillus casei on Kyse-30 Human Esophageal Cancer Cells

Subject Areas : Journal of Chemical Health Risks

Malihe Hashemi Khah ¹ , Nazila Arbab Soleymani ^{2 *} , Mohammad Mahdi Forghanifard ³ , Omid Gholami ⁴ , Saba Taheri ⁵

1 - Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran
2 - Department of Microbiology, Damghan Branch, Islamic Azad University, Damghan, Iran
3 - Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
4 - Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
5 - Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran

Received: 2022-08-16 Accepted : 2022-11-23 Published : 2023-09-01

Keywords: Probiotic, L.casei, Esophageal cancer, Viability assay,

Abstract :

One of the top causes of cancer-related deaths globally is esophageal cancer. In investigations of cell toxicity, the MTT test is one of the most often used cell viability/cytotoxicity assays for cellular metabolic activity. Nowadays, lactobacilli with probiotic effectiveness are now acknowledged as a prophylactic agent against cancer. The anti-tumor product of these bacteria have been designated in numerous studies. This investigation examined the probiotic Lactobacillus casei's in vivo impact on esophageal cancer. The MTT technique was used in this work to evaluate the cytotoxicity of L. casei (supernatant and full cell culture) to 5fu on the cancer cell line Kyse30. L. casei was able to decrease cell survival in supernatant and full cell culture (Kyse30). The possible impact of L. casei, particularly their supernatant, on esophageal cancer was initially evaluated in this research. As a result, lactobacilli species show promise for future research and development as cancer treatments.

References:

1. Liang H., Fan J.H., Qiao Y.L., 2017. Epidemiology, etiology, and prevention of esophageal squamous cell carcinoma in China. Cancer biology & medicine. 14(1), 33.


2. Izadpanah M.H., Abbaszadegan M.R., Fahim Y., Forghanifard M.M., 2017. Ectopic expression of TWIST1 upregulates the stemness marker OCT4 in the esophageal squamous cell carcinoma cell line KYSE30. Cellular & molecular biology letters. 22(1), 1-10.


3. Panda A., Bhanot G., Ganesan S., Bajpai M., 2021. Gene expression in Barrett’s esophagus cell lines resemble esophageal squamous cell carcinoma instead of esophageal adenocarcinoma. Cancers. 13(23), 5971.


4. Moghbeli M., Mosannen Mozaffari H., Memar B., Forghanifard M.M., Gholamin M., Abbaszadegan M.R., 2019. Role of MAML1 in targeted therapy against the esophageal cancer stem cells. Journal of Translational Medicine. 17(1), 1-12.


5. Gronnier C., Collet D., 2021. New Trends in Esophageal Cancer Management. MDPI. pp. 3030.

 

 

 

7. Tripathy A., Kancharla Dash S., Kolli P., Mahajan D., Senapati S., 2021. Probiotics: a promising candidate for management of colorectal cancer. Cancers. 13(13), 3178.


8. Śliżewska K., Markowiak-Kopeć P., Śliżewska W., 2020. The role of probiotics in cancer prevention. Cancers. 13(1), 20.


9. Kim S.J., Kang C.H., Kim G.H., Cho H., 2022. Anti-Tumor Effects of Heat-Killed L. reuteri MG5346 and L. casei MG4584 against Human Colorectal Carcinoma through Caspase-9-Dependent Apoptosis in Xenograft Model. Microorganisms. 10(3), 533.


10. Diba F.S., Hossain K.M., Azim M., 2013. Isolation, characterization and determination of antimicrobial properties of lactic acid bacteria from human milk. Jordan Journal of Biological Sciences. 147(624), 1-6.


11. Quinto E.J., Jiménez P., Caro I., Tejero J., Mateo J., Girbés T., 2014. Probiotic lactic acid bacteria: a review. Food and Nutrition Sciences. 5(18), 1765.


12. Wan L., Chen Z., Shah N., El-Nezami H., 2016. Modulation of intestinal epithelial defense responses by probiotic bacteria. Critical Reviews in Food Science and Nutrition. 56(16), 2628-2641.


13. Nami Y., Abdullah N., Haghshenas B., Radiah D., Rosli R., Khosroushahi A.Y., 2014. Assessment of probiotic potential and anticancer activity of newly isolated vaginal bacterium Lactobacillus plantarum 5BL. Microbiology and Immunology. 58(9), 492-502.


14. Bedada T.L., Feto T.K., Awoke K.S., Garedew A.D., Yifat F.T Birri D.J., 2020. Probiotics for cancer alternative prevention and treatment. Biomedicine & Pharmacotherapy. 129, 110409.


15. Lu K., Dong S., Wu X., Jin R., Chen H., 2021. Probiotics in cancer. Frontiers in Oncology. 11, 638148.


16. Karakaş D., Ari F., Ulukaya E., 2017. The MTT viability assay yields strikingly false-positive viabilities although the cells are killed by some plant extracts. Turkish Journal of Biology. 41(6), 919-925.


17. Fotakis G., Timbrell J.A., 2006. In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicology Letters. 160(2), 171-177.


18. George-Okafor U., Ozoani U., Tasie F., Mba-Omeje K., 2020. The efficacy of cell-free supernatants from Lactobacillus plantarum Cs and Lactobacillus acidophilus ATCC 314 for the preservation of home-processed tomato-paste. Scientific African. 8, e00395.


19. Koohestani M., Moradi M., Tajik H., Badali A., 2018. Effects of cell-free supernatant of Lactobacillus acidophilus LA5 and Lactobacillus casei 431 against planktonic form and biofilm of Staphylococcus aureus. in Veterinary Research Forum. Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.


20. Masebe R.D., Thantsha M.S., 2022. Anti-Biofilm Activity of Cell Free Supernatants of Selected Lactic Acid Bacteria against Listeria monocytogenes Isolated from Avocado and Cucumber Fruits, and from an Avocado Processing Plant. Foods. 11(18), 2872.


21. Cervantes-Elizarrarás A., Cruz-Cansino N.D.S., Ramírez-Moreno E., Vega-Sánchez V., Velázquez-Guadarrama N., Zafra-Rojas Q.Y., 2019. In vitro probiotic potential of lactic acid bacteria isolated from aguamiel and pulque and antibacterial activity against pathogens. Applied Sciences. 9(3), 601.


22. Jahangirian H., Haron M.J., Shah M.H., Abdollahi Y., Rezayi M., Vafaei N., 2013. Well diffusion method for evaluation of antibacterial activity of copper phenyl fatty hydroxamate synthesized from canola and palm kernel oils. Digest Journal of Nanomaterials & Biostructures. 8(3), 1263-1270.


23. Nazila A.S., Rooha K.K., Bagher Y., Taher N.S., 2010. Antagonistic activity of probiotic lactobacilli against Staphylococcus aureus isolated from bovine mastitis. African Journal of Microbiology Research. 4(20), 2169-2173.


24. Rad A., Farshchian M., Forghanifard M.M., Matin M.M., Bahrami A.R., Geerts D., 2016. Predicting the molecular role of MEIS1 in esophageal squamous cell carcinoma. Tumor Biology. 37(2), 1715-1725.


25. Zhang J., Zou S., Zhang Y., Yang Z., Wang W., Meng M., 2022. 3, 3′-Diindolylmethane Enhances Fluorouracil Sensitivity via Inhibition of Pyrimidine Metabolism in Colorectal Cancer. Metabolites. 12(5), 410.


26. Najafi M., Abbaszadegan M.R., Rad A., Dastpak M., Boroumand-Noughabi S., Forghanifard M.M., 2017. Crosstalk between SHH and stemness state signaling pathways in esophageal squamous cell carcinoma. Journal of cell communication and signaling. 11(2), 147-153.


27. Shi L.H., Balakrishnan K., Thiagarajah K., Ismail N.I.M., Yin O.S., 2016. Beneficial properties of probiotics. Tropical life sciences research. 27(2), 73.


28. Lee J.W., Shin J.G., Kim E.H., Kang H.E., Yim I.B., Kim J.Y., 2004. Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum. Journal of Veterinary Science. 5(1), 41-48.


29. Mandal S., 2018. Probiotic lactobacilli in microbiology research. Acta Scientific Microbiology. 1(1), 1-3.


30. Azam R., Ghafouri-Fard S., Tabrizi M., Modarressi M.H., Ebrahimzadeh-Vesal R., Daneshvar M., 2014. Lactobacillus acidophilus and Lactobacillus crispatus culture supernatants downregulate expression of cancer-testis genes in the MDA-MB-231 cell line. Asian Pacific Journal of Cancer Prevention. 15(10), 4255-4259.


31. Xanthopoulos V., Litopoulou-Tzanetaki E., Tzanetakis N., 2000. Characterization of Lactobacillus isolates from infant faeces as dietary adjuncts. Food Microbiology. 17(2), 205-215.


32. Patel M., Siddiqui A.J., Ashraf S.A., Surti M., Awadelkareem A.M., Snoussi M., 2022. Lactiplantibacillus plantarum-Derived Biosurfactant Attenuates Quorum Sensing-Mediated Virulence and Biofilm Formation in Pseudomonas aeruginosa and Chromobacterium violaceum. Microorganisms. 10(5), 1026.


33. Lee K.S., Yun E.Y., Goo T.W., 2020. Evaluation of the antimicrobial activity of an extract of Lactobacillus casei-infected Hermetia illucens larvae produced using an automatic injection system. Animals. 10(11), 2121.


34. Soltani N., Abbasi S., Baghaeifar S., Taheri E., Jadid M.F.S., Emami P., 2022. Antibacterial and antibiofilm activity of Lactobacillus strains secretome and extraction against Escherichia coli isolated from urinary tract infection. Biotechnology Reports. 36, e00760.


35. Sikorska H., Smoragiewicz W., 2013. Role of probiotics in the prevention and treatment of meticillin-resistant Staphylococcus aureus infections. International Journal of Antimicrobial Agents. 42(6), 475-481.


36. Malik S.S., Saeed A., Baig M., Asif N., Masood N., Yasmin A., 2018. Anticarcinogenecity of microbiota and probiotics in breast cancer. International Journal of Food Properties. 21(1), 655-666.