Kinetic study of DPPH scavenging in the presence of mixture of Zinc and Vitamin C as an antioxidant

Subject Areas : Journal of Chemical Health Risks

M. Momen Heravi ¹ , B. Haghi ² , A. Morsali ³ , P. Ardalan ⁴ , T. Ardalan ⁵

1 - Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran
2 - Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran
3 - Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran
4 - Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran, young researcher club
5 - Department of Chemistry, Islamic Azad University, Mashhad Branch, Mashhad, Iran, young researcher club

Received: 2012-08-06 Accepted : 2018-10-29 Published : 2012-06-01

Keywords: DPPH, Antioxidant, kinetic, Free radical, UV-Vis spectrometry,

Abstract :

Reactions of free radicals and reactive oxygen species (ROS) with biological molecules in vivo play an important physiological role in many diseases. 2,2-diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical and often used as a routine reagent to evaluate the antioxidant capacity of an antioxidant. This study was undertaken to investigate the free radical-scavenging and antioxidant activities of Zinc, Vitamin C and mixture of them. UV-Vis spectrophotometry method was used to evaluate the ability of Zinc, Vitamin C and mixture of them to scavenge DPPH radical. The kinetic parameters such as rate constant and activation energy in experimental conditions were calculated. The rate constants of the H atom abstraction by DPPH (), in the presence of Zinc, Vitamin C and mixture of them were obtained (0.4209, 2.092 and 1.82 min-1) respectively, under pseudo-first-order conditions at 25 oC.

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