Alleviation of the effects of on drought stress Verbascum nudicuale by methyl jasmonate and titanium dioxide nanoparticles

Subject Areas : Stress Physiology

Fatemeh Ghasemlou ¹ , Hamzeh Amiri ² , Roya Karamian ³ , Asghar Mirzaie-asl ⁴

1 - Department of Biology, Faculty of Science, Lorestan University, Lorestan, Iran
2 - Department of Biology, Faculty of Science, Lorestan University, Lorestan, Iran
3 - Department of Biology, Faculty of Science, Bu-Ali Sina University, Hamedan, Iran
4 - Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan

Received: 2019-11-04 Accepted : 2019-11-04 Published : 2019-08-01

Keywords: Drought stress, biomass, plant growth regulators, phenol, Verbascum nudicuale Wydl,

Abstract :

Drought stress causes severe metabolic dysfunctions by formation of oxidative stress that may lead to damage in DNA, inactivation of enzymes, and lipid peroxidation. Plants have developed different morphological, physiological, and biochemical mechanisms to withstand the drought stress. Plant growth regulators (PGRs) are one of the most important endogenous substances involved in the amelioration of tolerance in various plant species. In this study, the possible recovery ability of Verbascum nudicuale plants from drought stress conditions was assessed using methyl jasmonate (MJA), titanium dioxide nanoparticles (TiO2NPs), and their interactions as the PGRs in liquid culture media. Results showed that the growth parameters and photosynthetic pigment content markedly decreased due to polyethylene glycol (PEG)-induced drought stress. The treatment of V. nudicuale plants with TiO2NPs showed a considerably improving effect on pigments synthesis and biomass production during the treatment. However, cultures containing MJA negatively affected the growth parameters and increased the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA). Treatment of plants with PGRs showed a considerable improvement in increasing the synthesis of the phenolic compounds and proline accumulation. Alteration in the activity of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), and polyphenol oxidase (PPO) significantly varied in different treatments. These findings suggest that the recovery treatment with PGRs proved to be very effective in alleviating the adverse effects of drought stress.

References:

Abdel Latef, A. A., A. K. Srivastava, M. S.El‐sadek, M. Kordrostami and L. S. Tran.2018. 'Titanium dioxide nanoparticles improve growth and enhance tolerance of broad bean plants under saline soil conditions'. Land Degradation & Development, 29(4): 1065-73.

Akte, J., S.Yasmin, M. J. H.Bhuiyan, F. Khatun, J.Roy and K.Goswami.2016. 'In vitro screening of rice genotypes using polyethylene glycol under drought stress'. Progressive agriculture, 27(2): 128-135.

Ali, M.B., E. J. Hahn and K. Y. Paek .2007.'Methyl jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in Panax ginseng bioreactor root suspension cultures'. Molecules, 12: 607-621.

Alipieva, K. I., I. E. Orhan, I. I. T. Cankaya, E. P.Kostadinova and M. I.Georgiev.2014. 'Treasure from garden: chemical profiling, pharmacology and biotechnology of mulleins'. Phytochemistry Reviews, 13(2): 417-444.

Bates L., R. Waldren and I.Teare.1973.'Rapid determination of free proline for water-stress studies'. Plant Soil, 39: 205-207.

Beauchamp, C. and I.Fridovich.1971.'Superoxide dismutase: improved assays and an assay applicable to acrylamide gels'. Analytical Biochemistry, 44: 276-287.

Bradford, M.M. 1976.'A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding'. Analytical Biochemistry, 72: 248-254.

Chalker‐Scott, L. 1999.'Environmental significance of anthocyanins in plant stress responses'. Photochemistry and photobiology, 70: 1-9.

Chang, C. C., M. H,Yang, H. M.Wen and J. C.Chern.2002. 'Estimation of total flavonoid content in propolis by two complementary colorimetric methods'. Journal of Food and Drug Analysis, 10: 178–182.

Chiang, H. H. and A.Dandekar.1995. 'Regulation of proline accumulation in Arabidopsisthaliana(L.) Heynh during development and in response to desiccation'. Plant Cell and Environment, 18(11): 1280–1290.

Dimkpa, C. O., J. E.McLean, D. E.Latta, E.Manangón, D. W.Britt, W. P.Johnson.and Anderson, A. J. 2012. 'CuO and ZnO nanoparticles: phytotoxicity, metal speciation, and induction of oxidative stress in sand-grown wheat'. Journal of Nanoparticle Research, 14(1125): 1-15.

Gadzovska, S., S. Maury, A. Delaunay,M. Spasenoski, C. Joseph and D. Hagege. 2007. 'Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones'. Plant Cell, Tissue and Organ Culture, 89: 1-13.

González, L. F., M. C. Rojas and F. J. Perez.1999. 'Diferulate and lignin formation is related to biochemical differences of wall-bound peroxidases'. Phytochemistry, 50: 711-717.

Kamalizadeh, M., M. R. Bihamta, S. A. Peyghambari and J.Hadian.2015.'The effect of different levels of titanium dioxide nanoparticle on production of two majore phenolic compound in Dragonhead herb (Dracocephalum moldavica L.)'. Iranian Journal of Medicinal and Aromatic Plant, 31: 428-435.

Khanpour-Ardestani, N., M. Sharifi and M. Behmanesh. 2015.'Effect of methyl jasmonate on antioxidant enzyme activities, phenolic and flavonoid compounds in Scrophularia striata cell culture'. Journal of Plant Research, 27: 840–853.

Kus, M, W. Gernjak, S. M. RodrÃguez and S. Icli.2006. 'A comparative study of supported TiO2 as photocatalyst in water decontamination at solar pilot plant scale'. The Journal of Solar Energy Engineering, 128(3):331–337.

Lambert E., A. Faizal and D. Geelen.2011. 'Modulation of triterpene saponin production: in vitro cultures, elicitation, and metabolic engineering'. Applied Biochemistry and Biotechnology, 164: 220-237.

Lichtenthaler, H. K. and A. R. Wellburn.1983. 'Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents'. Portland Press Limited.

Maksymiec, W. 2007.'Signaling responses in plants to heavy metal stress'. Acta Physiologiae Plantarum, 29: 177-187.

Miliauskas, G., P. Venskutonis and T. Van Beek.2004. 'Screening of radical scavenging activity of some medicinal and aromatic plant extracts'. Food Chemistry, 85: 231-237.

Michel, B. E. and M. R. Kaufman.1973. 'The osmotic potential of polyethylene glycol 6000'. Plant Physiology 51: 914-916.

Murashige, T. and F.Skoog. 1962. 'A revised medium for rapid growth and bioassays with tobacco tissue cultures'. Physiologia Plantarum, 15: 473-497.

Nekrasova, G., O. A. Ushakova, M. Ermakov and I. Uimin and Byzov.2011.'Effects of copper (II) ions and copper oxide nanoparticles on Elodea densa Planch'. Russian Journal of Ecology, 42: 458-463.

Polevoi, V. 2001. 'The physiology of plant integrity'. Russian Journal of Plant Physiology, 48(4):545-555.

Popova, L. 2013.'Recent Advances and Future Prospects on Practical Use of Salicylic Acid. In: Hayat S, Ahmad A, Nasser M (eds) Salicylic Acid Plant Growth and Development'. Springer: Dordrecht, Netherlands, 357-385.

Raskin, I. 1992. 'Role of salicylic acid in plants'. Annual Review of Plant Biology, 43: 439-463.

Raymond, J., N. Rakariyatham and J. Azanza. 1993.'Purification and some properties of polyphenoloxidase from sunflower seeds'. Phytochemistry, 34: 927-931.

Shallan, M. A., H. M. Hassan, M. AA. Namich and A. A. Ibrahim.2016. 'Biochemical and Physiological Effects of TiO₂ and SiO₂ Nanoparticles on Cotton Plant under Drought Stress'. Research Journal of Pharmaceutical, Biological and Chemical Sciences (RJPBCS),7(4): 1541

Shan, C. and Z. Liang.2010.'Jasmonic acid regulates ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress'. Plant Science, 178: 130-139.

Shobha, G., V. Moses and S. Ananda. 2014.'Biological Synthesis of Copper Nanoparticles and its impact-a Review'. International Journal of Pharmaceutical Science Invention, 3: 6-28.

Velikova, V., I. Yordanov and A. Edreva. 2000.'Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines'. Plant Science, 151: 59-66.

Vos, C., H. Schat, M. Waal, R. Vooijs and W. Ernst. 1991.'Increased resistance to copper‐induced damage of the root cell plasmalemma in copper tolerant Silene cucubalus'. Physiologia Plantarum, 82: 523-528.

 

 

 

 

 

 

 

 

 

 

 Wu, H., X. Wu, Z. Li, L. Duan and M. Zhang. 2012. 'Physiological evaluation of drought stress tolerance and recovery in cauliflower (Brassica oleracea L.) seedlings treated with methyl jasmonate and coronatine'. Journal of Plant Growth Regulation, 31: 113-123.