Effect of Essential Oils of Various Plants as Microbial Modifier to Alter Corn Silage Fermentation and in vitro Methane Production
Subject Areas : Camel
ع. حجت پناه
1
,
م. دانش مسگران
2
*
,
A. وکیلی
3
,
ع.م. طهماسبی
4
1 - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
2 - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
3 - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
4 - Department of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran
Keywords: Essential Oil, methane emission, silage fermentation,
Abstract :
The aim of present study was to assess the effects of individual various essential plant oils on chemical composition, aerobic stability and fermentation characteristics of corn silage and in vitro rumen fermentation and methane emission using gas production technique. Whole crop corn was harvested and ensiled in laboratory scale tubes (4±0.25 kg) for 45 days. Treatments consisted of either corn silage with no additive (control) or treated with 120 or 240 mg kg-1 DM of cinnamon (Cinn120 or Cinn240, respectively), thyme (Thy120 or Thy240, respectively), mint (Mint120 or Mint240, respectively), oregano (Oreg120 or Oreg240, respectively) or cumin (Cum120 or Cum240, respectively). Treatments were compared with control using the Dunnett test at (P<0.05). Silages treated with cinnamon essential oil had higher DM content compared with the control (P<0.05). The pH value of Mint240 was significantly lower than the control (P<0.05). A significant reduction in ammonia nitrogen concentration of silages treated with thyme essential oil was observed in comparison to the control (P<0.05). Relative to the control, all of applied additives except of mint essential oil caused a significant improvement in silage aerobic stability (ranging from 44% to 400% increment). At 24 h in vitro rumen post incubation, total produced gas from Oreg240 was diminished significantly (P<0.05) compared with the control (44.23 vs. 48.73 mL 250 mg-1 DM). When compared to the control, all treated silages except of Mint240 or Oreg120 produced higher methane during 24 h incubation (P<0.05). However, the ratio of methane to total gas was not affected by experimental treatments (P>0.05). In vitro dry matter disappearance of Thy120, Mint240 or Oreg240 increased by 10 percent compared with the control (P<0.05). Moreover, partitioning factor of Oreg240 was higher than the control (P<0.05). Results demonstrated the potential of applied essential oils to improve chemical composition, dry matter degradability and aerobic stability of corn silage.
AOAC. (1990). Official Methods of Analysis. Vol. I. 15th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.
Balandrin M.F. and Klocke J.A. (1985). Natural plant chemicals: sources of industrial and medicinal materials. Science. 228, 1154-1160.
Beauchemin K.A. and McGinn S.M. (2006). Methane emissions from beef cattle: effects of fumaric acid, essential oil, and canola oil. J. Anim. Sci. 84, 1489-1496.
Benchaar C., Chaves A.V., Fraser G.R., Wang Y., Beauchemin K.A. and McAllister T.A. (2007). Effects of essential oils and their components on in vitro rumen microbial fermentation. Canadian J. Anim. Sci. 87, 413-419.
Benchaar C. and Greathead H. (2011). Essential oils and opportunities to mitigate enteric methane emissions from ruminants. Anim. Feed Sci. Technol. 166, 338-358.
Busquet M., Calsamiglia S., Ferret A. and Kamel C. (2005). Screening for the effects of natural plant extracts and secondary plant metabolites on rumen microbial fermentation in continuous culture. Anim. Feed Sci. Technol. 123, 597-613.
Chaves A.V., Baah J., Wang Y., McAllistera T.A. and Benchaar C. (2012). Effects of cinnamon leaf, oregano and sweet orange essential oils on fermentation and aerobic stability of barley silage. J. Sci. Food Agric. 92, 906-915.
Curtis O.F., Hetty K.S., Cassagnol G. and Peleg M. (1996). Comparison of the inhibitory and lethal effects of synthetic versions of plant metabolites (anethole, carvacrol, eugenol and thymol) on food spoilage yeast (Debaromyces hansenii). Food Biotechnol. 10, 55-73.
Dorman H.J.D. and Deans S.G. (2000). Antimicrobial agents from plants: antibacterial activity of plant volatile oils. J. Appl. Microbiol. 88, 308-316.
Dunière L., Sindou J., Chaucheyras-Durand F., Chevallierd I. and Thévenot-Sergentet D. (2013). Silage processing and strategies to prevent persistence of undesirable microorganisms. Anim. Feed Sci. Technol. 182, 1-15.
Duniere L., Gleizal A., Chaucheyras-Durand F., Chevallier I. and Thevenot-Sergentet D. (2011). Fate of Escherichia coli O26 in corn silage experimentally contaminated at ensiling, at opening or after aerobic exposure and protective effect of various bacterial inoculants. Appl. Environ. Microbiol. 77(24), 8696-8704.
Foskolos A., Cavini S., Rodriques-Prado M., Ferret A. and Calsamiglia S. (2010). A screening test of the use of essential oils compounds on ryegrass silage for preventing nitrogen losses in sustainable dairy production systems. Pp. 451-452 in Proc. 3rd EAAP Int. Symp. Energy and Protein. Metabol. Nutr. Parma, Italy.
Fraser G.R., Chaves A.V., Wang Y., McAllister T.A., Beauchemin K.A. and Benchaar C. (2007). Assessment of the effects of cinnamon leaf oil on rumen microbial fermentation using two continuous culture systems. J. Dairy Sci. 90, 2315-2328.
Hart K.J., Yanez-Ruiz D.R., Duval S.M., McEwan N.R. and Newbold C.J. (2008). Plant extracts to manipulate rumen fermentation. Anim. Feed Sci. Technol. 147, 8-35.
Ismaiel A. and Pierson M.D. (1990). Inhibition of growth and germination of C. botulinum 33A, 40B, and 1623E by essential oil of species. J. Food Sci. 55, 1676-1678.
Jahani-Azizabadi H., Danesh-Mesgaran M., Vakili A.R. and Rezayazdi K. (2014). Effect of some plant essential oils on in vitro ruminal methane production and on fermentation characteristics of a mid-forage diet. J. Agric. Sci. Technol. 16, 1543-1554.
Jahani-Azizabadi H., Danesh-Mesgaran M., Vakili A.R., Rezayazdi K. and Hashemi M. (2011). Effect of various semiarid native medicinal plant essential oils on rumen fermentation characteristics of a high forage diet using in vitro batch culture. African J. Microbiol. Res. 27, 4812-4819.
Khorrami B., Vakili A.R., Danesh Mesgaran M. and Klevenhusen F. (2015). Thyme and cinnamon essential oils: potential alternatives for monensin as a rumen modifier in beef production systems. Anim. Feed Sci. Technol. 200, 8-16.
Knowles J. and Roller S. (2001). Efficacy of chitosan, carvacrol and a hydrogen peroxide-based biocide against foodborne microorganisms in suspension and adhered to stainless steel. J. Food Prot. 64, 1542-1548.
Kung L.J. and Ranjit N.K. (2001). The effect of Lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. J. Dairy Sci. 84, 1149-1155.
Kung L.J., Williams P., Schmidt R.J. and Hu W. (2008). A blend of essential plant oils used as an additive to alter silage fermentation or used as a feed additive for lactating dairy cows. J. Dairy Sci. 91, 4793-4800.
Losa R. (2001). The use of essential oils in animal nutrition. In: Feed Manufacturing in the Mediterranean Region. Improving Safety: From Feed to Food. Pp.39-44 in Proc. 3rd Conf. Feed Manufactur. Mediterranean. Reus, Spain.
Macheboeuf D., Morgavi D.P., Papon Y., Mousset J.L. and Arturo-Schaan M. (2008). Dose–response effects of essential oils on in vitro fermentation activity of the rumen microbial population. Anim. Feed Sci. Technol. 145, 335-350.
McDonald P., Henderson A.R. and Heron S.J.E. (1991). The Biochemistry of Silage. Chalcombe Publications, Bucks, UK.
McIntosh F.M., Williams P., Losa R., Wallace R.J., Beever D.A. and Newbold C.J. (2003). Effects of essential oils on ruminal microorganisms and their protein metabolism. Appl. Environ. Microbiol. 69, 5011-5014.
Menke K.H. and Stenigass H. (1988). Estimation of the energetic feed value obtained from chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Dev. 28, 6-55.
Moran J.P., Weinberg Z.G., Ashbell G., Hen Y. and Owen T.R. (1996). A comparison of two methods for the evaluation of the aerobic stability of whole crop wheat silage. Pp. 162-163 in Proc. 11st Int. Silage Conf. Aberystwyth, UK.
Nanon A., Suksombat W. and Yang W.Z. (2014). Effects of essential oils supplementation on in vitro and in situ feed digestion in beef cattle. Anim. Feed Sci. Technol. 196, 50-59.
Navarro-Villa A., O'Brien M., López S., Boland T.M. and O'Kiely P. (2013). In vitro rumen methane output of grasses and grass silages differing in fermentation characteristics using the gas-production technique (GPT). Grass Forage. Sci. 68(2), 228-244.
Newbold C.J., McIntosh F.M., Williams P., Losa R. and Wallace R.J. (2004). Effects of a specific blend of essential oil compounds on rumen fermentation. Anim. Feed Sci. Technol. 114, 105-112.
Patra A.K. and Yu Z. (2014). Effects of vanillin, quillaja saponin, and essential oils on in vitro fermentation and protein-degrading microorganisms of the rumen. Appl. Microbiol. Biotechnol. 98, 897-905.
Roy D., Tomar S.K., Sirohi S.K., Kumar V. and Kumar M. (2014). Efficacy of different essential oils in modulating rumen fermentation in vitro using buffalo rumen liquor. Vet. World. 7(4), 213-218.
SAS Institute. (2002). SAS®/STAT Software, Release 9.2. SAS Institute, Inc., Cary, NC. USA.
Selwet M. (2009). Effect of propionic and formic acid mixtures on the fermentation, fungi development and aerobic stability of maize silage. Polish J. Agron. 1, 37-42.
Sliwinski B.J., Soliva C.R., Machmuller A. and Kreuzer M. (2002). Efficacy of plant extracts rich in secondary constituents to modify rumen fermentation. Anim. Feed Sci. Technol. 101, 101-114.
Theodorou M.K., Williams B.A., Dhanoa M.S., McAllan A.B. and France J. (1994). A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim. Feed Sci. Technol. 48, 185-197.
Van Soest P.J., Robertson J.B. and Lewis B.A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583-3597.
Voosogh A.S., Khomeiri M., Kashani Nijad M. and Jafari S.M. (2009). Effects of mint extract on the viability of probiotic bacteria in a native Iranian dairy drink (Doogh). J. Agric. Sci. Natur. Resour. 16(1), 156-165.
Walsh S.E., Maillard J.Y., Russell A.D., Catrenich C.E., Charbonneau D.L. and Bartolo R.G. (2003). Activity and mechanisms of action of selected biocidal agents on gram-positive and negative bacteria. J. Appl. Microbiol. 94, 240-427.
Yitbarek M.B. and Tamir B. (2014). Silage additives: a review. Open J. Appl. Sci. 4, 258-274.