Isolation and Characterization of Antimicrobial Activities of Native Actinomycete Strains from Agricultural Soils in Guilan province
Subject Areas : Biotechnological Journal of Environmental Microbiology
Elham Amiri
1
,
Mirsassan Mirpour
2
*
,
Khosro Essazadeh
3
,
Behnam Rasti
4
1 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران
2 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران
3 - گروه میکروبیولوژی ، دانشکده علوم پایه، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان ، ایران
4 - گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران
Keywords: Actinomycete, Antimicrobial activity, 16S rRNA, Amycolatopsis roodepoortensis, Streptomyces,
Abstract :
Actinomycetes are known as the largest Source of natural antibiotics in the world. For this reason, due to their
ability to produce various antibiotics and other compounds of therapeutic importance, they are considered the
golden microorganisms of the 21st century. The purpose of this research is the isolation and molecular identification
of Actinomycete with antimicrobial properties from agricultural soils in the native areas of Guilan province.
Soil samples were collected from the southwestern agricultural areas of Guilan province. Serial dilution
was used to isolate Actinomycetes. Then the morphological, physiological, and biochemical identification of
the samples was done, and finally, the molecular identification of the isolates was conducted using 16S rRNA
sequencing and phylogenetic analysis. Antimicrobial activity was investigated against pathogenic microorganisms.
A total of 14 isolates were identified, and two isolates with stronger antimicrobial properties were selected.
Based on the results of phylogenetic studies and 16S rRNA sequencing, Amycolatopsis roodepoortensis
strain EA7 was identified with 99.63% confidence, and Streptomyces microflaveus strain EA6 with 93.92%
confidence. The isolated bacteria exhibited higher antimicrobial activity against Gram-positive pathogenic
microorganisms such as Staphylococcus aureus and the standard sample Staphylococcus aureus PTCC 1112.
This research represents the first report on the identification of Actinomycetes with antimicrobial properties
in the agricultural soils of the southwestern regions of Guilan province, located in the Alborz mountains. The
identification of the rare strain of Amycolatopsis roodepoortensis strain EA7 from the northern regions of Iran
highlights the significant value of the soils in these regions.
Adamek M, Alanjary M, Sales-Ortells H, Goodfellow M, Bull A, Winkler A, Wibberg D, Kalinowski J, Ziemert N. Comparative genomics reveals phylogenetic distribution patterns of secondary metabolites in Amycolatopsis species.BMC Genomics .2018: 19(426):1-15.
Peng Q, Zhi-Xiang F, Jie-Wei T, Zu-Chao L, Lei W, Zhi-Gang Z, Yi-Wen C, Qiang T. Diversity, bioactivities, and metabolic potentials of endophytic actinomycetes isolated from traditional medicinal plants in Sichuan, China. Chinese Journal of Natural Medicines. 2015; 13(12): 0942- 0953.
Song Z, Xu T, Wang J, Hou Y, Liu C, Liu S, Wu S. Secondary Metabolites of the Genus Amycolatopsis: Structures, Bioactivities, and Biosynthesis.Molecules.2021;26(18):1-35.
Law JWF, Ser HL, Duangjai A, Sao Kaew S, Bukhari SI, Khan TM, et al. Streptomyces colonosanans sp. nov., a novel actinobacterium isolated from Malaysia mangrove soil exhibiting antioxidative activity and cytotoxic potential against human colon cancer cell lines. Frontiers in microbiology. 2017;8(1):877-891.
Takahashi Y, Nakashima T. Actinomycetes, an Inexhaustible Source of Naturally Occurring Antibiotics.Antibiotics Journal. 2018;7(45):1-17.
Ibnouf EO, Aldawsari MA, Waggiallah HA. Isolation and extraction of some compounds that act as antimicrobials from actinomycetes. Saudi Journal of Biological Sciences.2022; 29(8):1-7.
Tan LT-H, Chan K-G, Pusparajah P, Yin W-F, Khan TM, Lee L-H, et al. Mangrove-derived Streptomyces sp. MUM265 as a potential source of antioxidant and anti-cancer agents. BMC microbiology. 2019;19(1):1-16.
Kalaba MH, Moghannem SA, El-Hawary AS, Radwan AA, Sharaf MH, Shaban AS. Green synthesized ZnO nanoparticles mediated by Streptomyces plicatus: Characterizations, antimicrobial and nematicidal activities, and cytogenetic effects. Plants. 2021;10(9):1760-1786.
Ranjitha V, Ravishankar V. Extracellular synthesis of selenium nanoparticles from an actinomycetes Streptomyces griseoruber and evaluation of its cytotoxicity on HT-29 cell line. Pharmaceutical nanotechnology. 2018;6(1):61-68.
Fahmy NM, Abdel-Tawab AM. Isolation and characterization of marine sponge–associated Streptomyces sp. strain NMF6 producing secondary metabolite(s) possessing antimicrobial, antioxidant, anticancer, and antiviral activities. Journal of Genetic Engineering and Biotechnology. 2021;19(102):1-14.
Ansari MA, Alkubaisi N, Vijayaragavan P, Murugan P. Antimicrobial Potential of Streptomyces sp. To The Gram-Positive and Gram-Negative Pathogens. Journal of Infection and Public Health 2019; 12(1): 861-866.
Nabila MI, Kannabiran K. Biosynthesis, characterization and antibacterial activity of copper oxide nanoparticles (CuO NPs) from actinomycetes. Biocatalysis and agricultural biotechnology. 2018;15(1):56-62.
Oliveros KM,Rosana AR, Montecillo AD, Opulencia RB, Jacildo AJ, Zulaybar TO, Raymundo AK.Genomic Insights into the Antimicrobial and Anticancer Potential of Streptomyces sp. A1-08 Isolated from Volcanic Soils of Mount Mayon, Philippines. Philippine Journal of Science. 2021; 150 (6A): 1351-1377.
Osama N, Bakeer W, Raslan M, Soliman HA, Abdelmohsen UR, Sebak M. Anti-cancer and antimicrobial potential of five soil Streptomyces: a metabolomics-based study. Royal Society Open Science. 2022;9(2):1-17.
Li R, Wang M, Ren Z, Ji Y, Yin M, Zhou H, Tang SH-K. Amycolatopsis Aidingensis sp.nova., Halotolerant Actinobacterium, Produces New Secondary Metabolites. Frontiers in Microbiology.2021;12:1-14.
Kisil OV, Efimenko TA, Efremenkova OV. Looking Back to Amycolatopsis: History of the Antibiotic Discovery and Future Prospects. Antibiotics. 2021; 10(125): 1-25.
Kurnianto MA, Kusumaningrum HD, Lioe HN, Chasanah E. Antibacterial and antioxidant potential of ethyl acetate extract from Streptomyces AIA12 and AIA17 isolated from the gut of Chanos Chanos. BIODIVERSITAS .2021; 22(8):3196-3206.
Chen C, Ye Y, Wang R, Zhang Y, Wu C, Debnath S, Ma Z, Wang J, and Wu M. Streptomyces nigra sp. nov., is a novel actinobacterium isolated from mangrove soil and Exerts a Potent Antitumor Activity in Vitro. Frontiers in microbiology. 2018;9(1):1-14.
Alam M, Jha DK. Optimization of culture conditions for antimetabolite production by a rare tea garden actinobacterial isolate, Amycolatopsis sp. ST-28. African Journal of Clinical and Experimental Microbiology. 2019; 20 (3): 209-220.
Shaik M, Sankar G, Iswarya M, Rajitha P. Isolation and characterization of bioactivities of metabolites Producing marine Streptomyces parvulus Strain Sankarensis-A10. Journal Of Genetic Engineering and Biotechnology .2017;15,87-94.
Abd-Elnaby HM, Abo-Elala GM, Abdel-Raouf UM, Hamed MM. Antibacterial and anticancer activity of extracellular synthesized silver nanoparticles from marine Streptomyces rochei MHM13. The Egyptian Journal of Aquatic Research. 2016;42(3):301-312.
Nikbakht M, Omidi B, Amoozegar MA, Amini K. Cytotoxicity effect of secondary metabolites of Streptomyces Koyangensis and Streptomyces Tunisiensis isolated from saline soils of Garmsar City on human breast cancer cell line (MCF-7, IBRC C10082). Medical Science Journal of Islamic Azad Univesity-Tehran Medical Branch. 2021;31(4):367-376.
Sharma M, Manhas RK. Purification and characterization of actinomycins from Streptomyces strain M7 active against methicillin-resistant Staphylococcus aureus and vancomycin-resistant Enterococcus. BMC Microbiology. 2019;19(44):1-14.
Kawuri R, Darmayasa I. Bioactive Compound from Extract Filtrat Streptomyces sp. as Biocontrol of Vibriosis on Larvae of Macrobrachium Rosenbergii shrimps. Journal of Biosciences. 2019; 26(10):15-25.
Kumar R, Jadeja V. Isolation of Actinomycetes: A Complete Approach. International Journal of Current Microbiology and Applied Sciences. 2016;5(2): 606-618.
Gupta A, Singh D, Singh SK, Singh VK, Singh AV, Kumar A. Role of actinomycetes in bioactive and nanoparticle synthesis. Role of Plant Growth Promoting Microorganisms in Sustainable Agriculture and Nanotechnology: Elsevier. 2019; 4(1): 163-182.