In-vitro propagation of Pluchea lanceolata (DC) C.B.Clarke a potent antiarthritic medicinal herb through axillary bud

محورهای موضوعی : Medicinal and Herbal Plants

Satendra Singh ¹ , Ritu Mishra ² , Rajneesh Agnihotri ³

1 - Division of Genetics and Plant Breeding, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow -226015 (U.P.), India|Department of Botany, School of Life Science, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002(U.P.), India
2 - Division of Genetics and Plant Breeding, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow -226015 (U.P.), India|Academy of Scientific and Innovative Research (AcSIR), CSIR-Human Resource Development Centre Campus, Sector-19, Kamla Neh Nagar, Ghaziabad-201002 (U.P.), India
3 - Department of Botany, School of Life Science, Khandari Campus, Dr. B. R. Ambedkar University, Agra-282002(U.P.), India

تاریخ دریافت : 1399/04/09 تاریخ پذیرش : 1399/12/17 تاریخ انتشار : 1399/12/11

کلید واژه: PGRs, Axillaries bud explant, <i>Pluchea lanceolata</i>, MS medium, <i>In-vitro</i> propagation,

چکیده مقاله :

Pluchea lanceolata, commonly known as Rasana, is highly valued for its anti-inflammatory, antiarthritic, and analgesic properties and is extensively employed in treating rheumatoid arthritis, cough, neurological diseases, edema, sciatica, psoriasis, bronchitis, dyspepsia, and piles. Shrinking populations and insufficient variation due to rootstock propagation methods warrant efficient mass multiplication and conservation protocols. In the present study, explant (axillaries bud was cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of cytokinins and auxin (PGRs) individually, as well as in combination. The highest mean shoot number (2.06 ± 1.43) and second highest mean shoot length (2.62 ± 1.49 cm) were obtained in MS media enriched with 4 mg/L Benzyl amino purine (BAP) + 1mg/L Naphthalene acetic acid (NAA). Whereas, the best shoots multiplication (2.62 ± 1.41) and shoots elongation (5.88 ± 2.44) responses were displayed when MS medium supplemented with 0.25 mg/L (BAP) +0.50 mg/L Kinetin (KIN) were used.