تأثیر اینولین بر ویژگی‌های شیر تخمیری فراسودمند تولید شده توسط سویه‌های بومی جدا شده از ماست سنتی ایران

محورهای موضوعی : میکروبیولوژی مواد غذایی

فائزه شیرخان ¹ , سعید میردامادی ² , مهتا میرزایی ³ , بهروز اکبری آدرگانی ⁴ , نیکو نصوحی ⁵

1 - گروه علوم و صنایع غذایی، دانشکده داروسازی، علوم پزشکی تهران، دانشگاه آزاد اسلامی، تهران، ایران
2 - استاد پژوهشکده زیست فناوری، سازمان پژوهش‌های علمی و صنعتی ایران، تهران، ایران
3 - استادیار گروه علوم و صنایع غذایی، واحد شهر قدس، دانشگاه آزاد اسلامی، تهران، ایران
4 - عضو هیات علمی / سازمان غذا و دارو، آزمایشگاههای مرجع کنترل کیفی و ایمنی غذا
5 - استادیار دانشگاه علوم پزشکی آزاد اسلامی تهران، دانشکده علوم نوین، گروه بیوشیمی-بیوفیزیک، تهران، ایران

تاریخ دریافت : 1400/05/01 تاریخ پذیرش : 1400/10/02 تاریخ انتشار : 1401/04/01

کلید واژه: سویه‌های بومی, غذاهای فراسودمند, اینولین, دیابت, زمان نگهداری, شیر تخمیری,

چکیده مقاله :

مقدمه: با افزایش توجه مصرف‌کنندگان به محصولات غذایی با خواص حسی مناسب و دارای اثرات سلامت بخشی، تمایل به تولید محصولات لبنی فراسودمند در سال‌های اخیر افزایش یافته است.مواد و روش‌ها: در مطالعه حاضر برای تولید شیر تخمیری فراسودمند ابتدا اثر سویه‌های لاکتوباسیلوس دلبروکی، لاکتوباسیلوس هلوتیکوس جدا شده از ماست‌ سنتی ایران و کشت توأم دو سویه بر فعالیت ضد دیابتی شیر تخمیری بر اساس فعالیت مهارکنندگی آنزیم‌های آلفا آمیلاز و آلفا گلوکوزیداز بررسی شد. سپس اینولین به عنوان یک پری‌بیوتیک مناسب و کاربردی، به میزان 1 درصد به شیر تخمیری حاصل از ترکیب دو سویه اضافه و ویژگی‌های فیزیکوشیمیایی، ضد دیابتی، آنتی‌اکسیدانی، میزان پروتئولیز و جمعیت باکتری‌ها در طی 19 روز نگهداری در دمای 4 درجه سانتی‌گراد مورد ارزیابی قرار گرفت. یافته‌ها: نتایج نشان داد شیر تخمیر شده بوسیله کشت توأم دو سویه باکتریایی، فعالیت مهارکنندگی بیشتری بر آنزیم‌های آلفا آمیلاز (%45) و آلفا گلوکوزیداز (35%) نسبت به سویه‌های تکی لاکتوباسیلوس دلبروکی و لاکتوباسیلوس هلوتیکوس دارد (05/0p<) و افزودن اینولین باعث افزایش فعالیت مهارکنندگی آنزیم آلفا آمیلاز (20%) و آلفا گلوکوزیداز (33%) در زمان پایان تخمیر شد. ترکیب دو سویه باکتریایی و اینولین در طول دوره نگهداری در دمای 4 درجه سانتی‌گراد بطور معنی‌داری باعث افزایش جمعیت باکتری‌ها، اسیدیته، ویسکوزیته و کاهش معنی‌دار pH و میزان پروتئولیز شیر تخمیری گردید (05/0p<). با این وجود در طول این زمان کاهش فعالیت آنتی‌اکسیدانی و ضد دیابتی مشاهده گردید. نتیجه‌گیری: در مجموع نتایج نشان‌دهنده پتانسیل مناسب دو سویه باکتری به عنوان مایه تولید محصول تخمیری همراه با اینولین برای تولید محصول غذایی فراسومند بود.

چکیده انگلیسی:

Introduction: The inclination to manufacture beneficial dairy products has increased in recent years, as consumers pay more attention to food products with adequate sensory qualities and health benefits. Materials and Methods: The current study looked first at the anti-diabetic efficacy of milk fermented by Lactobacillus delbrueckii, and Lactobacillus helveticus strains isolated from traditional yogurt as well as co-culture of two-strain based on the inhibitory activity of alpha-amylase and alpha-glucosidase enzymes for the production of functional fermented milk. Then, added 1% inulin as an appropriate and applied prebiotic to fermented milk resulting from the combination of two strains and evaluated physicochemical, anti-diabetic, anti-oxidant, proteolysis rate, and population of bacteria during 19 days at 4°C.Results: The results indicated that milk fermented with two-strain bacterial intercropping had stronger inhibitory activity on alpha-amylase (45%) and alpha-glucosidase (35%) than single strains of Lactobacillus delbrueckii and Lactobacillus helveticus (p<0.05). The addition of inulin increased the inhibitory activity of alpha-amylase (20%) and alpha-glucosidase (33%) at the end of fermentation, respectively. During storage at 4°C, the composition two bacterial strains and inuline significantly increased the population of bacteria, acidity and viscosity and significantly reduced the pH and proteolysis rate of fermented milk. However, there was a decrease in antioxidant and antidiabetic activities. Conclusion: The results revealed that both bacterial strains in combination with inulin have the capacity to produce functional food.

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