نوشیدنی فراسودمند ترکیبی‌حاوی عصاره‌هایگیاهان خانواده چلیپائیان جهت بهبود سیستم ایمنی
محورهای موضوعی :
تغذیه -پروبیوتیک ها - غذاهای فراسودمند
زهرا لطیفی
1
(دانشجوی دکتری،گروه علوم و‌صنایع‌غذایی، واحد نور، دانشگاه آزاد اسلامی، نور، ایران)
سعید عابدیان کناری
2
(استاد، ایمنی‌شناسی پزشکی، دانشکده پزشکی، دانشگاه علوم پزشکیمازندران، ساری، ایران.)
علی اکبر مشایخ
3
(استادیار، گروه‌تربیت بدنی و‌علوم ورزشی، دانشکده علوم انسانی و اجتماعی، واحد نور، دانشگاه آزاد‌اسلامی، نور، ایران.)
تاریخ دریافت : 1400/10/18
تاریخ پذیرش : 1400/12/16
تاریخ انتشار : 1403/04/16
کلید واژه:
سیستم ایمنی,
عصاره گیاهی,
ظرفیت آنتی‌اکسیدانی,
گیاهان چلیپائیان,
خواص فیتوشیمیایی,
چکیده مقاله :
سیستم ایمنی بدن ارتباط تنگاتنگی با سلامت انسان دارد. بر این اساس، غذاهای فراسودمند حاوی ترکیبات فعال زیستی می‌توانند به‌عنوان بهبود دهنده سیستم ایمنی محسوب شوند. هدف از مطالعه حاضر تولید نوشیدنی فراسودمند ترکیبی، حاوی عصاره‌های گیاهان خانواده چلیپائیان (کلم سفید، کلم قرمز، کلم بروکلی و گل کلم) جهت بهبود سیستم ایمنی می باشد. بدین منظور عصاره‌های هیدرواتانولی (70 درصد) از چهار گونه کلم تهیه شد، سپس میزان ترکیبات فنولی و فلاونوئید کل و ظرفیت آنتی-اکسیدانی به روش DPPH بررسی گردید. سپس نوشیدنی‌هایی ترکیبی از چهار عصاره با غلظت های g/ml 400 و 800 تهیه گردید و موش‌های آزمایشگاهی با دوز یک سی‌سی، طی 15 روز متوالی تغذیه شدند. سیستم ایمنی با بررسی فاکتورهای IgG، IFN-γ، IL-4، IL-10 و IL-17 توسط روش الایزای غیر رقابتی ارزیابی شد. نتایج بدست آمده حاکی از بالا بودن فنل (mg GA/g 024/063/1) و فلاونوئید تام (mg QE/g 004/0426/0) در عصاره هیدرواتانولی کلم سفید بود. همچنین در روش DPPH، بالاترین میزان مهار کنندگی رادیکال آزاد مربوط به کلم قرمز به مقدار 26/98 درصد در غلظت g/ml 800 بود. نتایج ارزیابی سیستم ایمنی نشان داد که سه فاکتور IgG، IL-10 و IL-17 در موش‌ها، با مصرف نوشیدنی فراسودمند حاوی غلظت عصاره g/ml 800 افزایش یافت؛ به‌طوری که فاکتور IgG در گروه شاهد برابر با mg/ml 68/681/10 و در گروه سوم با غلظت عصاره g/ml 800 به mg/ml 86/2035/27 افزایش یافت. نتایج نشان داد که عصاره گیاهان خانواده چلیپائیان واجد پتانسیل بالایی برای استفاده در محصولات غذایی با هدف افزایش کیفیت و ارزش تغذیه ای را دارند.
منابع و مأخذ:
Ahmed, F. A. and. Ali, R. F., 2013. Bioactive compounds and antioxidant activity of fresh and processed white cauliflower. BioMed research international, 2013.
Amin, A.H., Bughdadi, F.A., Abo‐Zaid, M.A., Ismail, A.H., El‐Agamy, S.A., Alqahtani, A., El‐Sayyad, H.I., Rezk, B.M. and Ramadan, M.F., 2019. Immunomodulatory effect of papaya (Carica papaya) pulp and seed extracts as a potential natural treatment for bacterial stress. Journal of food biochemistry, 43, p.e13050.
Aziz, M. and. Karboune, S., 2018. Natural antimicrobial /antioxidant agents in meat and poultry products as well as fruits and vegetables: A review. Critical reviews in food science and nutrition, 58,486-511.
Bahorun, T., Luximon‐Ramma, A., Crozier, A. and. Aruoma, O. I., 2004. Total phenol, flavonoid, proanthocyanidin and vitamin C levels and antioxidant activities of Mauritian vegetables. Journal of the Science of Food and Agriculture, 84,1553-1561.
Barberis, C. L., Carranza, C. S., Magnoli, K., Benito, N. and. Magnoli, C.E., 2019. Development and removal ability of non-toxigenic Aspergillus section Flavi in presence of atrazine, chlorpyrifos and endosulfan. Revista Argentina de microbiologia, 51, 3-11.
Brindha, P. 2016. Role of phytochemicals as immunomodulatory agents: A review. International Journal of Green Pharmacy (IJGP), 10.
Chalamaiah, M., Yu, W. and. Wu, , 2018. Immunomodulatory and anticancer protein hydrolysates (peptides) from food proteins: A review. Food chemistry, 245,pp. 205-222.
Chauhan, H. and. Singh, M., 2019. Phytochemical characterization and antibacterial potential of Indian and Chinese cabbage genotypes against human pathogens in Uttarakhand, India International Journal of Recent Scientific Research, 17(12),pp. 36462-36466.
Cruz, A. B., Pitz, H. D. S., Veber, B., Bini, L. A., Maraschin, M. and. Zeni, A. L. B., 2016. Assessment of bioactive metabolites and hypolipidemic effect of polyphenolic-rich red cabbage extract. Pharmaceutical biology, 54, 3033-3039.
Deng, Z., Rong, Y., Teng, Y., Mu, J., Zhuang, X., Tseng, M., Samykutty, A., Zhang, L., Yan, J. and. Miller, D., 2017. Broccoli-derived nanoparticle inhibits mouse colitis by activating dendritic cell AMP-activated protein kinase. Molecular Therapy, 25, 1641-1654.
El-Desouky, W., Hanafi, A. and. Abbas, M. M., 2017. Radioprotective effect of green tea and grape seed extracts mixture on gamma irradiation induced immune suppression in male albino rats. International journal of radiation biology, 93, 433-439.
Ghasemi, K., Ghasemi, Y. and. Ebrahimzadeh, M. A., 2009. Antioxidant activity, phenol and flavonoid contents of 13 citrus species peels and tissues. Pak J Pharm Sci, 22, 277-281.
Ghoshal, G. & Kansal, S. K. 2019. The emerging trends in functional and medicinal beverage research and its health implication. Functional and medicinal beverages. Elsevier.
Hajmohammadi, A., Pirouzifard, M., Shahedi, M. and. Alizadeh, M., 2016. Enrichment of a fruit-based beverage in dietary fiber using basil seed: Effect of Carboxymethyl cellulose and Gum Tragacanth on stability. LWT, 74, pp. 84-91.
He, J. and. Giusti, M. M., 2010. Anthocyanins: natural colorants with health-promoting properties. Annual review of food science and technology, 1,163-187.
Horiguchi, N., Horiguchi, H. and. Suzuki, Y., 2005. Effect of wheat gluten hydrolysate on the immune system in healthy human subjects. Bioscience, biotechnology, and biochemistry, 69, 2445-2449.
Hussain, H., Al-Harrasi, A. and. Green, I. R. 2016. Frankincense (Boswellia) Oils. Essential Oils in Food Preservation, Flavor and Safety. Elsevier.
Jaafar, N. A., 2020. In vitro efficacy assessment of cauliflower (BrassicaoleraceaVar Botrytis) alcoholic extract in mortality percentage of motile stage of mite (Teranychusurticae Koch). Annals of Tropical Medicine and Health, 23,94-102.
Jaiswal, A. K., Abu‐Gahnnam, N. and. Gupta, S., 2012. A comparative study on the polyphenolic content, antibacterial activity and antioxidant capacity of different solvent extracts of Brassica oleracea International journal of food science & technology, 47, pp. 223-231.
Jaiswal, A. K., Rajauria, G., Abu- Gahnnam, N. and. Gupta, S., 2011. Phenolic composition, antioxidant capacity and antibacterial activity of selected Irish Brassica Natural Product Communications, 6, 1934578X1100600923.
Jakopic, J. and. Veberic, R., 2009. Extraction of phenolic compounds from green walnut fruits in different solvents. Acta Agriculturae Slovenica, 93, p. 11.
Jongen, W. 2002., Fruit and vegetable processing: Improving quality, Elsevier.
Jung, C.-H., Seog, H.-M., Choi, I.-W., Park, M.-W. and. Cho, H.-Y., 2006. Antioxidant properties of various solvent extracts from wild ginseng leaves. LWT-Food Science and Technology, 39, 266-274.
Kartal, N., Sokmen, M., Tepe, B., Daferera, D., Polissiou, M. and. Sokmen, A., 2007. Investigation of the antioxidant properties of Ferula orientalis using a suitable extraction procedure. Food chemistry, 100, pp.584-589.
Kaulmann, A., Andre, C. M., Schneider, Y.-J., Hoffmann, L. and. Bohn, T., 2016. Carotenoid and polyphenol bioaccessibility and cellular uptake from plum and cabbage varieties. Food chemistry, 197, 325-332.
Kaur, S. and. Mondal, P., 2014. Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants. J Microbiol Exp, 1, 00005.
Khan, S.R., Van Der Burgh, A.C., Peeters, R.P., van Hagen, P.M., Dalm, V.A. and Chaker, L., 2021. Determinants of Serum Immunoglobulin Levels: A Systematic Review and Meta-Analysis. Frontiers in immunology, 12, p.1103.
Kiewiet, M.B., Faas, M.M. and De Vos, P., 2018. Immunomodulatory protein hydrolysates and their application. Nutrients, 10, 904.
Koh, E., Wimalasiri, K., Chassy, A. and Mitchell, A., 2009. Content of ascorbic acid, quercetin, kaempferol and total phenolics in commercial broccoli. Journal of food composition and analysis, 22,637-643.
Kure, C., Timmer, J. and Stough, C., 2017. The immunomodulatory effects of plant extracts and plant secondary metabolites on chronic neuroinflammation and cognitive aging: a mechanistic and empirical review. Frontiers in pharmacology, 8,pp. 117.
Lippitz, B.E., 2013. Cytokine patterns in patients with cancer: a systematic review. The lancet oncology, 14,pp. 218-228.
Maier, T., Schieber, A., Kammerer, D.R. and Carle, R., 2009. Residues of grape
(Vitis vinifera L.) seed oil production as a valuable source of phenolic antioxidants. Food Chemistry, 112, pp.551-559.
Mao, R., Wu, L., Zhu, N., Liu, X., Hao, Y., Liu, R., Du, Q. and Li, Y., 2020. Immunomodulatory effects of walnut (Juglans regia) oligopeptides on innate and adaptive immune responses in mice. Journal of Functional Foods, 73, p.104068.
Mao, R., Wu, L., Zhu, N., Liu, X., Liu, R. and Li, Y., 2019. Naked Oat (Avena nuda) Oligopeptides: Immunomodulatory Effects on Innate and Adaptive Immunity in Mice via Cytokine Secretion, Antibody Production, and Th Cells Stimulation. Nutrients, 11, p. 927.
Marigoudar, S.R., Mohan, D., Nagarjuna, A. and Karthikeyan, P. 2018. Biomarker and histopathological responses of Lates calcarifer on exposure to sub lethal concentrations of chlorpyrifos. Ecotoxicology and environmental safety, 148, 327-335.
Marino, D., Ariz, I., Lasa, B., Santamaría, E., Fernández-Irigoyen, J., González-Murua, C. and Aparicio Tejo, P.M., 2016. Quantitative proteomics reveals the importance of nitrogen source to control glucosinolate metabolism in Arabidopsis thaliana and Brassica oleracea. Journal of experimental botany, 67, 3313-3323.
Oancea, S., Mila, L. and Ketney, O., 2019. Content of Phenolics, in vitro Antioxidant Activity and Cytoprotective Effects against Induced Haemolysis of Red Cabbage Extracts. Lett, 24, pp. 1-9.
Ordonez, A.A.L., Gomez, J.D. and Vattuone, M.A., 2006. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food chemistry, 97, 452-458.
Patel Rameshvar, K., Patel, M.M., Kanzariya, N.R., Vaghela, K.R., Patel, R.K. and Patel, N.J., 2010. In-vitro hepatoprotective activity of Moringa oleifera Lam. leave on isolated rat hepatocytes. j. ph. sci, 2, pp. 457-463.
Podsędek, A., 2007. Natural antioxidants and antioxidant capacity of Brassica vegetables: A review. LWT-Food Science and Technology, 40, 1-11.
Qaderi, M.M., Kurepin, L.V. and Reid, D.M., 2006. Growth and physiological responses of canola (Brassica napus) to three components of global climate change: temperature, carbon dioxide and drought. Physiologia Plantarum, 128, 710-721.
Raza, A., Hafeez, M.B., Zahra, N., Shaukat, K., Umbreen, S., Tabassum, J., Charagh, S., Khan, R.S.A. and Hasanuzzaman, M., 2020. The plant family Brassicaceae: Introduction, biology, and importance. The plant family Springer.
Rezq, A. A., 2017. Antioxidant Role of Cabbage (Brassica oleracea) Ethanolic Extract in Hepatoprotective of N-nitrosodiethylamine Induced Initiation of Hepatocellular Carcinoma in Rat Liver. Egyptian Journal of Nutrition, 32, 2.
Seong, G.U., Hwang, I.W. and Chung, S.K., 2016. Antioxidant capacities and polyphenolics of Chinese cabbage (Brassica rapa L. ssp. Pekinensis) leaves. Food Chemistry, 199, pp. 612-618.
Sethi, S., Tyagi, S.K. and Anurag, R.K., 2016. Plant-based milk alternatives an emerging segment of functional beverages: a review. Journal of food science and technology, 53, 3408-3423.
Shu, J., Liu, Y., Li, Z., Zhang, L., Fang, Z., Yang, L., Zhuang, M., Zhang, Y. and Lv, H., 2016. Detection of the diversity of cytoplasmic male sterility sources in broccoli (Brassica oleracea Italica) using mitochondrial markers. Frontiers in plant science, 7, p. 927.
Silambarasan, T., Manivannan, J., Raja, B. and Chatterjee, S., 2016. Prevention of cardiac dysfunction, kidney fibrosis and lipid metabolic alterations in L-NAME hypertensive rats by sinapic acid—Role of HMG-CoA reductase. European Journal of Pharmacology, 777, 113-123.
Singh, J., Upadhyay, A.K., Bahadur, A., Singh, B., Singh, K.P. and Rai, M., 2006. Antioxidant phytochemicals in cabbage (Brassica oleracea var. capitata). Scientia Horticulturae, 108,pp. 233-237.
Singh, J., Upadhyay, A.K., Prasad, K., Bahadur, A. and Rai, M.. 2007. Variability of carotenes, vitamin C, E and phenolics in Brassica Journal of Food Composition and Analysis, 20, pp.106-112.
Subramanian, V. and Gowry, S., 2011. Antitumor activity and antioxidant role of Brassica oleracea Italica against ehrlich ascites carcinoma in Swiss albino mice. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2,275-285.
Sultana, B., Anwar, F. and Ashraf, M., Effect of extraction solvent/technique on the antioxidant
activity of selected medicinal plant extracts. Molecules, 14, pp.2167-2180.
Sy, J.B.A., Hsu, T.C., Limaye, A. and Liu, J.R., 2020. Oral administration with a traditional fermented multi-fruit beverage modulates non-specific and antigen-specific immune responses in BALB/c mice. Plos one, 15, e0233047.
Thomas, M., Badr, A., Desjardins, Y., Gosselin, A. and Angers, P., 2018. Characterization of industrial broccoli discards (Brassica oleracea italica) for their glucosinolate, polyphenol and flavonoid contents using UPLC MS/MS and spectrophotometric methods. Food chemistry, 245, pp. 1204-1211.
Tometten, M., Blois, S., Kuhlmei, A., Stretz, A., Klapp, B.F. and Arck, P.C., 2006. Nerve growth factor translates stress response and subsequent murine abortion via adhesion molecule-dependent pathways. Biology of reproduction, 74, 674-683.
Toy, D., Kugler, D., Wolfson, M., Bos, T.V., Gurgel, J., Derry, J., Tocker, J. and Peschon, J., 2006. Cutting edge: interleukin 17 signals through a heteromeric receptor complex. The Journal of Immunology, 177, 36-39.
Udenigwe, C.C., Lu, Y.L., Han, C.H., Hou, W.C. and Aluko, R.E., 2009. Flaxseed protein-derived peptide fractions: Antioxidant properties and inhibition of lipopolysaccharide-induced nitric oxide production in murine macrophages. Food Chemistry, 116, 277-284.
Verma, A.K., Pathak, V., Singh, V.P. and Umaraw, P., 2016. Storage study of chicken meatballs incorporated with green cabbage (Brassica olerecea) at refrigeration temperature (4±1oC) under aerobic packaging. Journal of applied animal research, 44, 409-414.
Yimit, D., Hoxur, P., Amat, N., Uchikawa, K. and Yamaguchi, N. 2012. Effects of soybean peptide on immune function, brain function, and neurochemistry in healthy volunteers. Nutrition, 28,154-159.
Zhao, L., Zhang, Y., Liu, G., Hao, S., Wang, C. and Wang, Y., 2018. Black rice anthocyanin-rich extract and rosmarinic acid, alone and in combination, protect against DSS-induced colitis in mice. Food & function, 9,2796-2808.
_||_
