بررسی شیوع پاتوژن‌های غذازاد در ساندویچ‌های عرضه‌شده در شهرستان قم و مقاومت آنتی‌بیوتیکی جدایه‌ها
محورهای موضوعی :
بهداشت مواد غذایی
سید عرفان حسینی نسب
1
,
نجمه واحد دهکردی
2
,
ابراهیم رحیمی
3
1 - دانش‌آموخته بهداشت مواد غذایی، گروه بهداشت مواد غذایی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
2 - دانشجوی دکترای تخصصی بهداشت مواد غذایی، گروه بهداشت مواد غذایی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
3 - استاد تمام گروه بهداشت مواد غذایی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
تاریخ دریافت : 1402/05/05
تاریخ پذیرش : 1402/08/08
تاریخ انتشار : 1402/07/01
کلید واژه:
قم,
مقاومت آنتی‌بیوتیکی,
آلودگی باکتریایی,
گاستروانتریت,
ساندویچ,
چکیده مقاله :
امروزه به دلیل وجود مشاغل متفاوت امکان پخت غذا برای تمام افراد یک جامعه وجود ندارد و بخش عمده ای از مردم به سمت استفاده غذاهای آماده روی آورده اند، که در کنار تمام مزایایی که دارد، در صورت عدم رعایت بهداشت می توانند منبع مهمی از آلودگی های باکتریایی باشند که سبب شیوع گاستروانتریت شوند. در همین راستا هدف از مطالعه حاضر بررسی شیوع پاتوژن های غذازاد در ساندویچ های عرضه شده در شهرستان قم و مقاومت آنتی بیوتیکی جدایه ها بود. تعداد 120 نمونه شامل 30 نمونه از سمبوسه، سالاد، ناگت سنتی مرغ، همبرگر سنتی از مراکز عرضه شهرستان قم به صورت تصادفی جداسازی و در کنار فلاسک یخ جهت جلوگیری از آلودگی ثانویه به آزمایشگاه بهداشت مواد غذایی دانشگاه آزاد شهرکرد انتقال داده شد و توسط روش های استاندارد مورد ارزیابی قرار گرفت. نتایج نشان داد که میزان آلودگی در مواد غذایی به آرکوباکتر 83/28، سودوموناس 65/16، باسیلوس سرئوس 35/23 و برای کمپیلوباکتر 75/14 درصد بوده است که بیشترین و کمترین میزان آلودگی به ترتیب برای آرکوباکتر و کمپیلوباکتر بود. بیشترین میزان مقاومت به آنتی بیوتیک ها مربوط به باسیلوس سرئوس با 56/78 درصد و کمترین مقاومت مربوط به کمپیلوباکتر با 7/53 درصد بود. بنابراین توصیه می شود مصرف مواد غذایی خیابانی به حداقل مصرف برسد و در صورت ابتلا به گاستروانتریت ناشی از آلودگی های غذایی، استفاده از آنتی بیوتیک ها کاهش یابد.
چکیده انگلیسی:
Nowadays, due to the existence of different occupations, it is not possible to cook food for all the people of a society, and most of the people have turned to using ready-made foods, which, in addition to all its advantages, can be a source of They are important bacterial contaminations that cause the spread of gastroenteritis. In this regard, the aim of this study is to investigate the prevalence of Arcobacter, Pseudomonas, Campylobacter and Bacillus cereus in sandwiches offered in Qom city and the antibiotic resistance of the isolates. 120 samples, including 30 samples of samosas, salads, traditional chicken nuggets, traditional hamburgers from the supply centers of Qom city, were randomly separated and transported to the food hygiene laboratory of Shahrekord Azad University along with ice flasks to prevent secondary contamination. The standards were evaluated. The results showed that Arcobacter 28.83%, Pseudomonas 16.65%, Bacillus cereus 23.35% and Campylobacter 14.75% were the highest and lowest contamination rates for Arcobacter and Campylobacter respectively. The highest level of resistance to antibiotics was related to Bacillus cereus with 78.56% and the lowest resistance was related to Campylobacter with 53.7%. Therefore, it is recommended to reduce the consumption of street food to a minimum, and in case of gastroenteritis caused by food contamination, the use of antibiotics should also be minimized.
منابع و مأخذ:
Abay, S., Irkin, R., Aydin, F., Müştak, H.K., Diker, K.S. )2017(. The prevalence of major foodborne pathogens in ready-to-eat chicken meat samples sold in retail markets in Turkey and the molecular characterization of the recovered isolates. LWT-Food Science and Technology, 81(2): 202-209.
Abdul-Mutalib, N., Syafinaz, A., Sakai, K., Shirai, Y. )2015(. An overview of foodborne illness and food safety in Malaysia. International Food Research Journal, 22 (3): 896-901.
Chon, J.-W., Yim, J.-H., Kim, H.-S., Kim, D.-H., Kim, H, et al., )2015(. Quantitative prevalence and toxin gene profile of Bacillus cereus from ready-to-eat vegetables in South Korea. Foodborne Pathogens and Disease, 12 (1): 795-799.
Cossettini, A., Vidic, J., Maifreni, M., Marino, M., Pinamonti, D., Manzano, M. )2022(. Rapid detection of Listeria monocytogenes, Salmonella, Campylobacter spp., and Escherichia coli in food using biosensors. Food Control, 137 (4): 108962.
Di Noto, A.M., Sciortino, S., Cardamone, C., Ciravolo, C., Napoli, C., Alio, , et al., )2018(. Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study. Italian Journal of Food Safety, 7 (3): 72-75
Elmanama, A.A., Albayoumi, M.A. )2016(. High prevalence of antibiotic residues among broiler chickens in gaza strip. Food and Public Health, 6(4): 93-98.
Ferreira, S., Oleastro, M., Domingues, F. )2019(. Current insights on Arcobacter butzleri in food chain. Current Opinion in Food Science, 26 (1): 9-17.
Ghotaslou, R., Leylabadlo, H.E., Asl, Y.M. )2015(. Prevalence of antibiotic resistance in Helicobacter pylori: A recent literature review. World Journal of Methodology, 5 (4): 164- 171.
Hakeem, M.J., Lu, X. )2021(. Survival and control of Campylobacter in poultry production environment. Frontiers in Cellular and Infection Microbiology, 10(5): 615-449.
Hanashiro, A., Morita, M., Matté, G.R., Matté, M.H., Torres, E.A. )2005(. Microbiological quality of selected street foods from a restricted area of Sao Paulo city, Brazil. Food control, 16(7): 439-444.
Heidarzadi, M., Rahnama, M., Alipoureskandani, M., Saadati, D., Afsharimoghadam, A. )2021(. Salmonella and Escherichia coli contamination in samosas presented in Sistan and Baluchestan province and antibiotic resistance of isolates. Food Hygiene, 11(2): 81-90.[In Persian]
Hwang, J.-, Park, J.-H. )2015(. Characteristics of enterotoxin distribution, hemolysis, lecithinase, and starch hydrolysis of Bacillus cereus isolated from infant formulas and ready-to-eat foods. Journal of Dairy Science, 98(3): 1652-1660.
Ishola, O., Mosugu, J., Adesokan, H. )2016(. Prevalence and antibiotic susceptibility profiles of Listeria monocytogenes contamination of chicken flocks and meat in Oyo State, south-western Nigeria: Public health implications. Journal of Preventive Medicine and Hygiene, 57(2): 157-163
Jain, R., Aras, R. )2007(. Depression in geriatric population in urban slums of Mumbai. Indian Journal of Public Health, 51(4): 112-113.
Lupo, A., Haenni, M., Madec, J.-Y. )2018(. Antimicrobial resistance in Acinetobacter spp. and Pseudomonas spp. Microbiology Spectrum, 6(3): 63- 73.
Mottola, A., Bonerba, E., Bozzo, G., Marchetti, P., Celano, G.V., Colao, et al., )2016(. Occurrence of emerging food-borne pathogenic Arcobacter spp. isolated from pre-cut (ready-to-eat) International Journal of Food Microbiology, 236(2): 33-37.
Murindamombe, G.Y., Collison, E.K., Mpuchane, S.F., Gashe, B.A. )2005(. Presence of Bacillus cereus in street foods in Gaborone, Botswana. Journal of Food Protection, 68(5): 342-346.
Nguyen, P., Juarez, O., Restaino, L. )2021(. A new method for detection of Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii using a novel chromogenic agar. Journal of Food Protection, 84(3): 160-168.
Nonato, I.L., Minussi, L.d.A., Pascoal, G.B., De-Souza, D.A. )2016(. Nutritional issues concerning street foods. Journal of Clinical Nutrition Diet, 2(1): 1-7.
Ntushelo, K., Ledwaba, L.K., Rauwane, M.E., Adebo, O.A., Njobeh, P.B. )2019(. The mode of action of Bacillus species against Fusarium graminearum, tools for investigation, and future prospects. Toxins, 11(5): 606-614.
Odewale, G., Jibola-Shittu, M.Y., Omosule, N.S., Esan, T.B. )2023(. Multidrug resistant bacteria associated with a fresh fruit and vegetables sold in Lokoja market, Kogi State, Nigeria. Microbes and Infectious Diseases, 12(3): 115-125.
Pinu, F.R. )2016(. Early detection of food pathogens and food spoilage microorganisms: Application of metabolomics. Trends in Food Science & Technology, 54(5): 213-215.
Ramarao, N., Tran, S.-L., Marin, M., Vidic, J. )2020(. Advanced methods for detection of Bacillus cereus and its pathogenic factors. Sensors, 20(9): 1-23.
Sansinenea, E. )2019(. Bacillus spp.: As plant growth-promoting bacteria. Secondary metabolites of plant growth promoting rhizomicroorganisms: Discovery and Applications, 225-237.
Tabashsum, Z., Khalil, I., Nazimuddin, M., Mollah, A., Inatsu, Y., Bari, M.L. )2013(. Prevalence of foodborne pathogens and spoilage microorganisms and their drug resistant status in different street foods of Dhaka city. Agriculture Food and Analytical Bacteriology, 3(4): 281-292.
Vindigni, S.M., Srijan, A., Wongstitwilairoong, B., Marcus, R., Meek, J., Riley, P.L., et al., )2007(. Prevalence of foodborne microorganisms in retail foods in Thailand. Foodborne Pathogens and Disease, 4(5): 208-215.
Yu, S., Yu, P., Wang, J., Li, C., Guo, H., Liu, et al., )2020(. A study on prevalence and characterization of Bacillus cereus in ready-to-eat foods in China. Frontiers in Microbiology, 10(1): 1-23.
Zhuang, L., Li, Y., Wang, Z., Yu, Y., Zhang, N., Yang, C., et al., )2021(. Synthetic community with six Pseudomonas strains screened from garlic rhizosphere microbiome promotes plant growth. Microbial Biotechnology, 14(2): 488-502.
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Abay, S., Irkin, R., Aydin, F., Müştak, H.K., Diker, K.S. )2017(. The prevalence of major foodborne pathogens in ready-to-eat chicken meat samples sold in retail markets in Turkey and the molecular characterization of the recovered isolates. LWT-Food Science and Technology, 81(2): 202-209.
Abdul-Mutalib, N., Syafinaz, A., Sakai, K., Shirai, Y. )2015(. An overview of foodborne illness and food safety in Malaysia. International Food Research Journal, 22 (3): 896-901.
Chon, J.-W., Yim, J.-H., Kim, H.-S., Kim, D.-H., Kim, H, et al., )2015(. Quantitative prevalence and toxin gene profile of Bacillus cereus from ready-to-eat vegetables in South Korea. Foodborne Pathogens and Disease, 12 (1): 795-799.
Cossettini, A., Vidic, J., Maifreni, M., Marino, M., Pinamonti, D., Manzano, M. )2022(. Rapid detection of Listeria monocytogenes, Salmonella, Campylobacter spp., and Escherichia coli in food using biosensors. Food Control, 137 (4): 108962.
Di Noto, A.M., Sciortino, S., Cardamone, C., Ciravolo, C., Napoli, C., Alio, , et al., )2018(. Detection of Arcobacter spp. in food products collected from Sicilia region: A preliminary study. Italian Journal of Food Safety, 7 (3): 72-75
Elmanama, A.A., Albayoumi, M.A. )2016(. High prevalence of antibiotic residues among broiler chickens in gaza strip. Food and Public Health, 6(4): 93-98.
Ferreira, S., Oleastro, M., Domingues, F. )2019(. Current insights on Arcobacter butzleri in food chain. Current Opinion in Food Science, 26 (1): 9-17.
Ghotaslou, R., Leylabadlo, H.E., Asl, Y.M. )2015(. Prevalence of antibiotic resistance in Helicobacter pylori: A recent literature review. World Journal of Methodology, 5 (4): 164- 171.
Hakeem, M.J., Lu, X. )2021(. Survival and control of Campylobacter in poultry production environment. Frontiers in Cellular and Infection Microbiology, 10(5): 615-449.
Hanashiro, A., Morita, M., Matté, G.R., Matté, M.H., Torres, E.A. )2005(. Microbiological quality of selected street foods from a restricted area of Sao Paulo city, Brazil. Food control, 16(7): 439-444.
Heidarzadi, M., Rahnama, M., Alipoureskandani, M., Saadati, D., Afsharimoghadam, A. )2021(. Salmonella and Escherichia coli contamination in samosas presented in Sistan and Baluchestan province and antibiotic resistance of isolates. Food Hygiene, 11(2): 81-90.[In Persian]
Hwang, J.-, Park, J.-H. )2015(. Characteristics of enterotoxin distribution, hemolysis, lecithinase, and starch hydrolysis of Bacillus cereus isolated from infant formulas and ready-to-eat foods. Journal of Dairy Science, 98(3): 1652-1660.
Ishola, O., Mosugu, J., Adesokan, H. )2016(. Prevalence and antibiotic susceptibility profiles of Listeria monocytogenes contamination of chicken flocks and meat in Oyo State, south-western Nigeria: Public health implications. Journal of Preventive Medicine and Hygiene, 57(2): 157-163
Jain, R., Aras, R. )2007(. Depression in geriatric population in urban slums of Mumbai. Indian Journal of Public Health, 51(4): 112-113.
Lupo, A., Haenni, M., Madec, J.-Y. )2018(. Antimicrobial resistance in Acinetobacter spp. and Pseudomonas spp. Microbiology Spectrum, 6(3): 63- 73.
Mottola, A., Bonerba, E., Bozzo, G., Marchetti, P., Celano, G.V., Colao, et al., )2016(. Occurrence of emerging food-borne pathogenic Arcobacter spp. isolated from pre-cut (ready-to-eat) International Journal of Food Microbiology, 236(2): 33-37.
Murindamombe, G.Y., Collison, E.K., Mpuchane, S.F., Gashe, B.A. )2005(. Presence of Bacillus cereus in street foods in Gaborone, Botswana. Journal of Food Protection, 68(5): 342-346.
Nguyen, P., Juarez, O., Restaino, L. )2021(. A new method for detection of Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii using a novel chromogenic agar. Journal of Food Protection, 84(3): 160-168.
Nonato, I.L., Minussi, L.d.A., Pascoal, G.B., De-Souza, D.A. )2016(. Nutritional issues concerning street foods. Journal of Clinical Nutrition Diet, 2(1): 1-7.
Ntushelo, K., Ledwaba, L.K., Rauwane, M.E., Adebo, O.A., Njobeh, P.B. )2019(. The mode of action of Bacillus species against Fusarium graminearum, tools for investigation, and future prospects. Toxins, 11(5): 606-614.
Odewale, G., Jibola-Shittu, M.Y., Omosule, N.S., Esan, T.B. )2023(. Multidrug resistant bacteria associated with a fresh fruit and vegetables sold in Lokoja market, Kogi State, Nigeria. Microbes and Infectious Diseases, 12(3): 115-125.
Pinu, F.R. )2016(. Early detection of food pathogens and food spoilage microorganisms: Application of metabolomics. Trends in Food Science & Technology, 54(5): 213-215.
Ramarao, N., Tran, S.-L., Marin, M., Vidic, J. )2020(. Advanced methods for detection of Bacillus cereus and its pathogenic factors. Sensors, 20(9): 1-23.
Sansinenea, E. )2019(. Bacillus spp.: As plant growth-promoting bacteria. Secondary metabolites of plant growth promoting rhizomicroorganisms: Discovery and Applications, 225-237.
Tabashsum, Z., Khalil, I., Nazimuddin, M., Mollah, A., Inatsu, Y., Bari, M.L. )2013(. Prevalence of foodborne pathogens and spoilage microorganisms and their drug resistant status in different street foods of Dhaka city. Agriculture Food and Analytical Bacteriology, 3(4): 281-292.
Vindigni, S.M., Srijan, A., Wongstitwilairoong, B., Marcus, R., Meek, J., Riley, P.L., et al., )2007(. Prevalence of foodborne microorganisms in retail foods in Thailand. Foodborne Pathogens and Disease, 4(5): 208-215.
Yu, S., Yu, P., Wang, J., Li, C., Guo, H., Liu, et al., )2020(. A study on prevalence and characterization of Bacillus cereus in ready-to-eat foods in China. Frontiers in Microbiology, 10(1): 1-23.
Zhuang, L., Li, Y., Wang, Z., Yu, Y., Zhang, N., Yang, C., et al., )2021(. Synthetic community with six Pseudomonas strains screened from garlic rhizosphere microbiome promotes plant growth. Microbial Biotechnology, 14(2): 488-502.
