Application of response surface methodology (RSM) for the optimization of supercritical CO2 extraction of Elaeagnus protein: a comparison study
Subject Areas : food science
Sepideh Hesamizadeh
1
,
S. Askari
2
,
محمدرضا حسین پور
3
1 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات
2 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات
3 - دانشگاه آزاد اسلامی، واحد علوم و تحقیقات
Keywords: Elaeagnus angustifolia, Supercritical CO2 extraction, Plant proteins, Essential amino acids, Gel electrophoresis,
Abstract :
Elaeagnus contains a large amount of protein which can be used as a functional ingredient. In this study, dried fruit protein was extracted using supercritical carbon dioxide method. Extraction was performed at different supercritical temperatures, supercritical pressure, and solvent ratio of carbon dioxide and methanol. According to the ANOVA results, the optimum operating condition for the extraction of protein with supercritical CO2 was as following:150.85 bar, 51.06°C, 59.80 min and methanol aqueous solvent concentration of 717.08 µL, leading to extracting of 47.53 mg protein per 10 g sample. Compared to other extraction methods, the amount of extracted protein was high with this method. In addition, less damage was done to other physical-chemical properties of the protein, including protein structure, amino acid chains, etc. Among the operating conditions, supercritical pressure had the highest effect on proteins. Therefore, the results of this study suggested that the supercritical carbon dioxide extraction can be sued as an effective method for protein extraction from plants.
Abizov, A.E., Tolkachev, O.N., Mal'tsev, S.D. & Abizova, E.V. (2007). Composition of Biologicaly active substances isolated from the Russian olive (Elaeagnus angustifolia) introduced in the European part of Russia. Pharmaceutical Chemistry Journal, 42, 23-25. https://doi.org/10.1007/s11094-009-0203-5
Biligic S., Cubuk N.S.Y., Demirkoz A.B. & Karakas M. (2019). Response surface optimization and modelling for supercritical carbon dioxide extraction of Echium Valgare seed oil. Journal of Supercritical Fluid, 143, 365-369. https://doi.org/10.1016/j.supflu.2018.09.008
Corzzini, A., Soyari, C.S., Helena, D.F.Q., Barros, A., Renato, A.G., Fernando, A. (2017). Extraction of edible Avacado oil using supercritical Co2 and Co2/ethanol mixture as solvents. Journal af Food Engineering, 194, 40-45. https://doi.org/10.1016/j.jfoodeng.2016.09.004
Hamidpour, R., Hamidpour, S., Hamidpour, M., Shahlari, M., Sohrabu, M., Shahlari, N. & Hamidpour, R.O. (2017). Russian olive (Elaeagnus angustifolia L.): From a variety of traditional medicional applications to its novel roles as active antioxidant, anti-inflammatory,anti-mutagenic and analgesic agent. Journal of Traditional and Complentary Medicine, 7, 24-29. https://doi.org/10.1016/j.jtcme.2015.09.004
Honarvar, M., Varaee, M., Eikani, M.H., Omidkhah, M.R. & Moraki, N. (2019). Supercritical fluid extraction of free amino acids from sugar beet and sugar cane molasses. Journal of Supercritical Fluids, 144, 48-55. https://doi.org/10.1016/j.supflu.2018.10.007
Jing, W.Ch., Jian, B.X. & Ming, X. (2007). Supercritical fluid CO2 extraction of essential oil from Marchantia Convoluta: Global jields and extract chemical composition. Electronic Journal of Biotechnology, 10, 1. https://doi.org/10.4067/S0717-34582007000100013
Kim, W., Wang, Y. & Selonulya, C. (2020). Dairy and plant proteins as neutral food emulsifiers. Trend and Food Science & Technology,105, 261-272. https://doi.org/10.1016/j.tifs.2020.09.012
Kosterzewa, D. & Dobrzynska, A.I. (2020). Optimization of supercritical carbon dioxide extraction of sweet paprika (Capsicum annuum L.) using response surface methodology. Chemical Engineering Research and Design, 160, 39-51. https://doi.org/10.1016/j.cherd.2020.05.005
Lima, N.R., Ribeiro, S.A., Cardozo, F.L., Vedo, J., D. & Alves, B.P. (2019). Extraction from leaves of piper klotszchianum using supercritical carbon dioxide and co-solvent. Journal of Supercritical Fluids, 44, 205-212. https://doi.org/10.1016/j.supflu.2018.11.006
Michele, C., Mesomoa, B., Marcos, L., Carazzaa, P. M., Nadiayea, O.R., Cardozoc, D. & Sheeraa, A.P. (2013). Supercritical Co2 extracts and essential oil of ginger (Zingiber officinale R.): Chemical composition and antibacterial activity. Journal of Supercritical Fluids, 80, 44-49. https://doi.org/10.1016/j.supflu.2013.03.031
Pedro, T.W., Barrosoa, P. P., Carvalhob, D., Thiago, B., Rochab, F., Debora, A. & Azevedoc, M. F. (2016). Evaluation of the composition of Carica papaya L. seed oil extracted with supercritical CO2. Journal of Biotechnology, 11, 110-116. https://doi.org/10.1016/j.btre.2016.08.004
Preece, K.E., Hooshyar, N. & Zuidam, N.J. (2017). Whole soybean protein extraction processes: A review. Innovative Food Science and Emerging Technologies, 43,163-172. https://doi.org/10.1016/j.ifset.2017.07.024
Sahena, F., Zaidul, I.S.M., Jinap, S., Karim, A., Abbasi, K.A., Norulaini, N.A. & Omar, A.K.M. (2009). Application of supercritical Co2 in lipid extraction- A review. Journal of Food Engineering, 95, 240-253. https://doi.org/10.1016/j.jfoodeng.2009.06.026
Soares, J.F., Dalpra, V., Souza, M., Lunelli, F.C., Abaide, E., Silva, J.R.F., Kuhn, R.C. & Martimez, J. (2016). Extraction of Rice bran oil using supercritical CO2 and compressed liquefied petroleum gad. Journal of Food Engineering,170, 58-63. https://doi.org/10.1016/j.jfoodeng.2015.09.016
Valle, J.M., Veronica, G. & Mertine, J.L. (2012). Supercritical CO2 extraction of allicin from Garlic flakes: Screening and kinetic studies. Food Research International Journal, 45, 216-224. https://doi.org/10.1016/j.foodres.2011.10.021
Wen, Ch., Zhang, J., Zhang, H., Daun, Y. & Ma, H., (2020), Plant protein - devired antioxidant peprides: Isolation - Identification mechanism of action and application in food system: A review. Trend and Food Science and Technology Journal, 105, 308-322. https://doi.org/10.1016/j.tifs.2020.09.019
Xiong, K. & Chen, Y., (2020), Supercritical carbon dioxide extraction of essential oil from Tangerine Peel: Experimental optimization and kinetics modelling, Chemical Engineering Research and Design, 164, 412-423. https://doi.org/10.1016/j.cherd.2020.09.032.