بررسی اثر عصاره چای سبز (Camellia sinensis) بر سندرم تخمدان پلی‌کیستیک القایی به وسیله لتروزول در موش‌های صحرایی ماده بالغ نژاد ویستار
محورهای موضوعی :
آسیب شناسی درمانگاهی دامپزشکی
سید ایمان خدارحمی
1
,
سید پژمان مرتضوی
2
,
اکرم عیدی
3
1 - کارشناس ارشد زیست‌شناسی سلولی و تکوینی، گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
2 - دانشیار گروه پاتولوژی، دانشکده دامپزشکی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
3 - استاد گروه زیست‌شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران.
تاریخ دریافت : 1402/02/05
تاریخ پذیرش : 1402/06/03
تاریخ انتشار : 1402/08/01
کلید واژه:
آنتی‌اکسیدان,
عصاره چای سبز,
لتروزول,
سندرم تخمدان پلی‌کیستیک,
چکیده مقاله :
چای سبز (Camellia sinensis) از لحاظ زیست شیمیایی و خواص دارویی همواره در طب سنتی مورد توجه قرارگرفته، دارای فعالیت آنتی اکسیدانی در برابر استرس اکسیداتیو می باشد. سندرم تخمدان پلی کیستیک (polycystic ovary syndrome; PCOS)، اختلال شایع غدد درون ریز است که برخی از زنان در سنین باروری به آن مبتلا می شوند. هدف مطالعه حاضر، بررسی اثر عصاره چای سبز بر PCOS القایی، توسط لتروزول در موش صحرایی بود. بدین منظور 48 سر موش صحرایی ماده بالغ نژاد ویستار به طور تصادفی در 8 گروه تقسیم شدند. حیوانات گروه کنترل سالم در طول آزمایش تیماری دریافت نکرد. موش های گروه کنترل PCOS، لتروزول را در دوز 1 میلی گرم برکیلوگرم وزن بدن، به صورت گاواژ دریافت کردند. همچنین حیوانات سه گروه تجربی سالم عصاره چای سبز را در دوزهای 50، 100 و 200 میلی گرم برکیلوگرم وزن بدن به صورت گاواژ دریافت کردند و موش های سه گروه تجربی PCOS هم علاوه بر لتروزول، عصاره چای سبز را در 3 دوز مذکور به صورت گاواژ دریافت کردند. در پایان این آزمایش در روز 28، حیوانات آسان‌کشی شده و آنزیم های آنتی اکسیدانی سوپر اکسید دیسموتاز، کاتالاز و گلوتاتیون پراکسیداز و همچنین پراکسیداسیون لیپیدی در هموژنات بافت تخمدان مورد سنجش قرار گرفت. عصاره چای سبز باعث افزایش معنی دار فعالیت آنزیم های آنتی اکسیدانی و کاهش معنی دار پراکسیداسیون لیپیدی گردید (001/0 p<). نتایج نشان داد که عصاره چای سبز، با افزایش فعالیت آنزیم های آنتی اکسیدانی، در درمان PCOS ناشی از لتروزول در موش صحرایی موثراست.
چکیده انگلیسی:
Green tea (Camellia sinensis), has always been a focus of interest in traditional medicine for its biochemical and medicinal properties as an antioxidant agent. Polycystic ovary syndrome (PCOS) is a common endocrinopathy among women of reproductive age. In the present study, the effects of green tea extract on letrozole-induced PCOS were evaluated. Forty eight adult female Wistar rats were assigned to eight groups in random: healthy control group (no treatment), PCOS control group (letrozole at 1 mg/kg by gavage), three healthy experimental groups (green tea extract at 50, 100 and 200 mg/kg body weight by gavage), and three experimental PCOS groups (green tea extract at 50, 100 and 200 mg/kg body weight + letrozole at 1 mg/kg by gavage). On the 28th day (at the end of the experiment), the rats were euthanized, and the antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) and lipid peroxidation were assessed in the ovarian tissue homogenates. The results indicated that the green tea extract significantly increased the activities of the antioxidant enzymes and decreased lipid peroxidation (p<0.001). Our results showed that the green tea extract was also effective in treating letrozole-induced PCOS in the rats by increasing the activities of the antioxidant enzymes.
منابع و مأخذ:
Abdolahi, M., Khorsandi, L. and Ahrari, (2010). The protective effect of green tea extract on acetaminophen induced nephron – toxicity in mice. Journal of Arak University of Medical Sciences, 13(1): 90-96. [In Persian]
Abolfathi, A., Rezaie, A., Mousavi, G., Valilou, M. and Jafari, B. (2011). Effects of Green tea extract on liver histopathology and serum biomarkers of hepatic tissue injury in streptozotocin-induced diabetic rats. Journal of Veterinary Clinical Pathology, 5(19): 1315-1324. [In Persian]
Alchami, A., O’Donovan, O. and Davies, M. (2015). PCOS: diagnosis and management of related infertility. Obstetrics. Gynaecology and Reproductive Medicine, 25(10): 279-282.
Amoughli Tabrizi, B. and Mohajeri, D. (2014). Preventive Effects of Green Tea Extract from Hepatic Steatosis in the Rats Fed with High Fat Diet. Journal of Rafsanjan University of Medical Sciences, 13(2): 125-140. [In Persian]
Asadi, S., Zamiri, A., Ezzati, S., Parsaei, P., Rafieian, M. and Shirzad, F. (2011). Effect of alcoholic extract of green tea (Camellia sinensis) on the healing process in surgical and burn wounds in rats. Journal of Birjand University of Medical Sciences, 18(1): 1-9. [InPersian]
Azam, S., Hadi, N., Khan, N.U. and Hadi, S.M. (2004). Proxidiantproeperty of green tea polyphenols, epicatechin and epicatechin-3-gallate: implications of anticancer properties. Journal of Toxicology in Vitro, 18(5): 555-561.
Choi, S.J., Park, S.Y., Park, J.S., Park, S.K. and Jung, M.Y. (2016). Contents and compositions of policosanols in green tea (Camellia sienesis) leaves. Journal of Food Chemistry, 204(1): 94-101.
Chobot, V., Huber, CH., Trettenhahn, G. and Hadacek, F. (2009). Catechin: Chemical Weapon, Antioxidant, or Stress Regulator. Journal of Chemical Ecology, 35(8): 980-96.
Crespy, V. and Williamson, G. (2004). A review of the health effects of green tea catechins in in vivo animal models. Journal of Nutrition, 134(12): 3431-40.
Dikmen, A., Ergenoglu, A.M., Yeniel, A.O., Dilsiz, O.Y., Ercan, G. and Yilmaz, H. (2012). Evaluation of glycemic and oxidative/antioxidative status in the estradiol valerate-induced PCOS model of rats. European Journal of Obstetrics & Gynecology and Reproductive Biology, 160(1): 55-59.
Duleba, AJ. (2012). Medical management of metabolic dysfunction in PCOS. Journal of Steroids, 77(4): 306-11.
Gadkari, PV. and Balaraman, M. (2015). Green tea: Sources, extraction and encapsulation: A review. Journal of Food and Bioproducts Processing, 93(1): 122-38.
Ghafurniyan, H., Azarnia, M., Nabiuni, M. and Karimzadeh, L. (2015). The effect of green tea extract on reproductive improvement in estradiol valerate – induced polycystic ovarian syndrome in rat. Iranian Journal of Pharmaceutical Research, 14(4): 1215-1233.
Goswami, PK., Khale, A., and Ogale, S. (2012). Natural remedies for polycystic ovarian syndrome (PCOS). A review. International Journal of Pharmaceutical and Phytopharmacological Research, 1(6): 396-402.
Hasegawa, R., Chujo, T., Sai-Kato, K., Umemura, T., Tanimura, A. and Kurokawa, Y. (2000). Preventive effects of green tea against liver oxidative DNA damage and hepatotoxicity in rats treated with 2-intropropane. Journal of Food and Chemical Toxicology, 33(11): 961-70.
Ishii, T., Mori, T., Tanaka, T., Mizuno, D., Yamaji, R., Kumazawa, SH., et al. (2008). Covalent modification of proteins by green tea polyphenol- epigallocatechin-3-gallate through autoxidation. Journal of Free Radical Biology and Medicine, 45(1): 1384-94.
Katiyar, S., Elmetsa, CA. and Katiyar, SK. (2007). Green tea and skin cancer: Photoimmunology, angiogensis and DNA repair. Journal of Nutritional Biochemistry, 18(5): 287-96.
Lambert, JD. and Elias, RJ. (2010). The antioxidant and prooxidant activities of green tea polyphenols: a role in cancer prevention. Archives of Biochemistry and Biophysics Journal, 50(1): 65-72.
Malhotra, N., Gongadashetti, K., Daha, R. and Singh, N. (2014). Oxidative stress biomarkers in follicular fluid of women with PCOS and tubal factor infertility-is there a correlation with in-vitro-fertilization outcome. International Journal of Fertility and Sterility, 102(3): 86-87.
Mohajeri, D., Doustar, Y. and Rahmani, J. (2011). Antioxidant activity of Green tea extract against Isoniazid induced hepatotoxicity in the rats. Journal of Veterinary Clinical Pathology, 5(18): 1221-1231. [In Persian]
Montazeri, H., Safavi, M., Ebrahim - Habibi, A. and Yaghmaei, P. (2018). The effects of Betanin of Beetroot in ploy cystic ovary syndrome induced rat. Journal of Medicinal Plants, 17(68): 74-81. [In Persian]
Naderpoor, N., Shorakae, S., de Courten, B., Misso, M.L., Moran, L.J. and Teede, H.J. (2015). Metformin and lifestyle modification in polycystic ovary syndrome: systematic review and met-analysis. Journal of Human Reproduction Update, 21(5): 560-74.
Norouzi, T., Ghatreh Samani, K., Amini, S.A., Jafarzadeh, L. and Mardani, G. (2016). Compare the effects of atorvastatin and omega-3 on index of lipid oxidation in patients with polycystic ovary syndrome. Journal of Shahrekord University of Medical Sciences, 18(1): 36-44. [In Persian]
Rasoulifard, M. and Zargari, F. (2015). The effect of aqueous extract of white tea on serum antioxidant enzymes in rats exposed to arsenic. Journal of Veterinary Clinical Pathology, 9(34): 153-161. [In Persian]
Sabuncu, T., Vural, H. and Harma, M. (2001). Oxidative stress in polycystic ovary syndrome and its contribution to the risk of cardiovascular disease. Journal of Clinical Chemistry, 34(5): 407-13.
Sadat Khorsandi, L., Javadnia, F., Orazizadeh, M. and Abdolahi, M. (2010). Effect of green tea (Camellia sinesis L.) extract on acetaminophen induced acute hepatoxicity in mice. Iranian Journal of Medicinal and Aromatic Plants Research, 26(1): 22-9. [In Persian]
Senanayake, S.P.J.N. (2013). Green tea extract: Chemistry, antioxidant properties and food applications. A review. Journal of Functional Foods, 5(4): 1529-41.
Shariatzadeh, MA. and Mohammadi, M. (2015). Protective role of green tea (Camellia sinensis) hydroalcholic extract on sperm parameters and testicular tissue in NMRI mice exposed to sodium arsentie. Journal of Birjand University of Medical Sciences, 21(4): 432-43. [In Persian]
Tahmasebi, F., Movahedin, M. and Mazaheri, Z. (2015). Poly Cystic Ovary Model as an Elevated Oxidative Stress Factor. Journal of Mazandaran University of Medical Sciences, 25(127): 82-91. [In Persian]
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Abdolahi, M., Khorsandi, L. and Ahrari, (2010). The protective effect of green tea extract on acetaminophen induced nephron – toxicity in mice. Journal of Arak University of Medical Sciences, 13(1): 90-96. [In Persian]
Abolfathi, A., Rezaie, A., Mousavi, G., Valilou, M. and Jafari, B. (2011). Effects of Green tea extract on liver histopathology and serum biomarkers of hepatic tissue injury in streptozotocin-induced diabetic rats. Journal of Veterinary Clinical Pathology, 5(19): 1315-1324. [In Persian]
Alchami, A., O’Donovan, O. and Davies, M. (2015). PCOS: diagnosis and management of related infertility. Obstetrics. Gynaecology and Reproductive Medicine, 25(10): 279-282.
Amoughli Tabrizi, B. and Mohajeri, D. (2014). Preventive Effects of Green Tea Extract from Hepatic Steatosis in the Rats Fed with High Fat Diet. Journal of Rafsanjan University of Medical Sciences, 13(2): 125-140. [In Persian]
Asadi, S., Zamiri, A., Ezzati, S., Parsaei, P., Rafieian, M. and Shirzad, F. (2011). Effect of alcoholic extract of green tea (Camellia sinensis) on the healing process in surgical and burn wounds in rats. Journal of Birjand University of Medical Sciences, 18(1): 1-9. [InPersian]
Azam, S., Hadi, N., Khan, N.U. and Hadi, S.M. (2004). Proxidiantproeperty of green tea polyphenols, epicatechin and epicatechin-3-gallate: implications of anticancer properties. Journal of Toxicology in Vitro, 18(5): 555-561.
Choi, S.J., Park, S.Y., Park, J.S., Park, S.K. and Jung, M.Y. (2016). Contents and compositions of policosanols in green tea (Camellia sienesis) leaves. Journal of Food Chemistry, 204(1): 94-101.
Chobot, V., Huber, CH., Trettenhahn, G. and Hadacek, F. (2009). Catechin: Chemical Weapon, Antioxidant, or Stress Regulator. Journal of Chemical Ecology, 35(8): 980-96.
Crespy, V. and Williamson, G. (2004). A review of the health effects of green tea catechins in in vivo animal models. Journal of Nutrition, 134(12): 3431-40.
Dikmen, A., Ergenoglu, A.M., Yeniel, A.O., Dilsiz, O.Y., Ercan, G. and Yilmaz, H. (2012). Evaluation of glycemic and oxidative/antioxidative status in the estradiol valerate-induced PCOS model of rats. European Journal of Obstetrics & Gynecology and Reproductive Biology, 160(1): 55-59.
Duleba, AJ. (2012). Medical management of metabolic dysfunction in PCOS. Journal of Steroids, 77(4): 306-11.
Gadkari, PV. and Balaraman, M. (2015). Green tea: Sources, extraction and encapsulation: A review. Journal of Food and Bioproducts Processing, 93(1): 122-38.
Ghafurniyan, H., Azarnia, M., Nabiuni, M. and Karimzadeh, L. (2015). The effect of green tea extract on reproductive improvement in estradiol valerate – induced polycystic ovarian syndrome in rat. Iranian Journal of Pharmaceutical Research, 14(4): 1215-1233.
Goswami, PK., Khale, A., and Ogale, S. (2012). Natural remedies for polycystic ovarian syndrome (PCOS). A review. International Journal of Pharmaceutical and Phytopharmacological Research, 1(6): 396-402.
Hasegawa, R., Chujo, T., Sai-Kato, K., Umemura, T., Tanimura, A. and Kurokawa, Y. (2000). Preventive effects of green tea against liver oxidative DNA damage and hepatotoxicity in rats treated with 2-intropropane. Journal of Food and Chemical Toxicology, 33(11): 961-70.
Ishii, T., Mori, T., Tanaka, T., Mizuno, D., Yamaji, R., Kumazawa, SH., et al. (2008). Covalent modification of proteins by green tea polyphenol- epigallocatechin-3-gallate through autoxidation. Journal of Free Radical Biology and Medicine, 45(1): 1384-94.
Katiyar, S., Elmetsa, CA. and Katiyar, SK. (2007). Green tea and skin cancer: Photoimmunology, angiogensis and DNA repair. Journal of Nutritional Biochemistry, 18(5): 287-96.
Lambert, JD. and Elias, RJ. (2010). The antioxidant and prooxidant activities of green tea polyphenols: a role in cancer prevention. Archives of Biochemistry and Biophysics Journal, 50(1): 65-72.
Malhotra, N., Gongadashetti, K., Daha, R. and Singh, N. (2014). Oxidative stress biomarkers in follicular fluid of women with PCOS and tubal factor infertility-is there a correlation with in-vitro-fertilization outcome. International Journal of Fertility and Sterility, 102(3): 86-87.
Mohajeri, D., Doustar, Y. and Rahmani, J. (2011). Antioxidant activity of Green tea extract against Isoniazid induced hepatotoxicity in the rats. Journal of Veterinary Clinical Pathology, 5(18): 1221-1231. [In Persian]
Montazeri, H., Safavi, M., Ebrahim - Habibi, A. and Yaghmaei, P. (2018). The effects of Betanin of Beetroot in ploy cystic ovary syndrome induced rat. Journal of Medicinal Plants, 17(68): 74-81. [In Persian]
Naderpoor, N., Shorakae, S., de Courten, B., Misso, M.L., Moran, L.J. and Teede, H.J. (2015). Metformin and lifestyle modification in polycystic ovary syndrome: systematic review and met-analysis. Journal of Human Reproduction Update, 21(5): 560-74.
Norouzi, T., Ghatreh Samani, K., Amini, S.A., Jafarzadeh, L. and Mardani, G. (2016). Compare the effects of atorvastatin and omega-3 on index of lipid oxidation in patients with polycystic ovary syndrome. Journal of Shahrekord University of Medical Sciences, 18(1): 36-44. [In Persian]
Rasoulifard, M. and Zargari, F. (2015). The effect of aqueous extract of white tea on serum antioxidant enzymes in rats exposed to arsenic. Journal of Veterinary Clinical Pathology, 9(34): 153-161. [In Persian]
Sabuncu, T., Vural, H. and Harma, M. (2001). Oxidative stress in polycystic ovary syndrome and its contribution to the risk of cardiovascular disease. Journal of Clinical Chemistry, 34(5): 407-13.
Sadat Khorsandi, L., Javadnia, F., Orazizadeh, M. and Abdolahi, M. (2010). Effect of green tea (Camellia sinesis L.) extract on acetaminophen induced acute hepatoxicity in mice. Iranian Journal of Medicinal and Aromatic Plants Research, 26(1): 22-9. [In Persian]
Senanayake, S.P.J.N. (2013). Green tea extract: Chemistry, antioxidant properties and food applications. A review. Journal of Functional Foods, 5(4): 1529-41.
Shariatzadeh, MA. and Mohammadi, M. (2015). Protective role of green tea (Camellia sinensis) hydroalcholic extract on sperm parameters and testicular tissue in NMRI mice exposed to sodium arsentie. Journal of Birjand University of Medical Sciences, 21(4): 432-43. [In Persian]
Tahmasebi, F., Movahedin, M. and Mazaheri, Z. (2015). Poly Cystic Ovary Model as an Elevated Oxidative Stress Factor. Journal of Mazandaran University of Medical Sciences, 25(127): 82-91. [In Persian]
