فهرست مقالات Pre-germination

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  1 - The Effect of Gibberellic Acid and Stratification on Germination of Alstroemeria (Alstroemeria ligtu hybrid) Seed In Vitro and In Vivo Conditions
  Fardin Nasri Nasser Ghaderi Jaafar Mohammadi Seyed Najmedin Mortazavi Mahmood Koshesh Saba
  The dormancy characteristics and optimum conditions for seed germination of Alstroemeria ligtu had not been explained. In vitro and in vivo alstroemeria (A. ligtu hybrid) seed germination tests were conducted in a Randomized Completely Design at two different treatments چکیده کامل

  The dormancy characteristics and optimum conditions for seed germination of Alstroemeria ligtu had not been explained. In vitro and in vivo alstroemeria (A. ligtu hybrid) seed germination tests were conducted in a Randomized Completely Design at two different treatments (gibberellic acid (GA3) 0, 100, 200 and 400 mg/l with and without stratification in 5±1oC) in four replications. Seeds were planted in the soil mixture (peat/sand/perlite 1:1:1) or 1.2 MS media (1% sucrose, 0.7% agar and pH to 5.8). After 3-weeks keeping in the stratification conditions, transferred to the growth chamber (21oC and 16h photoperiod). Shoot and root length, number of root and leaf, root and shoot fresh and dry weight, seed germination percentage, germination rate and mean germination time were recorded during experiment. Stratification had a significant effect on seed germination (p<0.05). Soaking for 24 h in 100 mg/l GA3 supplemented with stratification under in vitro and in vivo conditions increased germination up to 76.67% and 70.00%, respectively. Mean germination time (MGT) decreased with duration of stratification and concentration of GA3. Seeds treated with 100 mg.l-1 GA3 plus 21 days of stratification produced the seedlings with the higher number of leaf, length of shoot, shoot and root dry weight in both In vivo and in vitro conditions. Non-stratified seeds without GA3 application fail to germinate, whereas seeds chilled for 21 days had 36.6%, 40.0% germination under in vivo and in vitro, respectively. Stratification was successful in breaking seed dormancy; stratification at 5±1oC for 21 days or 100 mg/l GA3+21 days of stratification overcame seed dormancy and increased the germination percentage of A.ligtu hybrid seeds. Thus, seeds of A.ligtu hybrid species probably exhibit a combination of physiological dormancy. In general, In vivo germination rates were lower than in vitro rates. پرونده مقاله
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  2 - Effects of Pre-germination Treatment on the Phytate and Phenolic Contents of Almond Nuts
  Liang Lin Lin Freda Xin You Giam Xin Min Foo Nadia Marie Hui Lian Yeo Charlene Jia Ling Koh Nur Hatika Binte Sa’Aban Wai Mun Loke
  10.22034/jon.2017.530394
  This study examined if pre-germination altered the water content and water activity, contents of phytate, total phenolic, (±)-catechin, quercetin and total antioxidant capacity of almond (Prunus dulcis)kernel. Raw almond kernels were submerged for 15 hours in wat چکیده کامل

  This study examined if pre-germination altered the water content and water activity, contents of phytate, total phenolic, (±)-catechin, quercetin and total antioxidant capacity of almond (Prunus dulcis)kernel. Raw almond kernels were submerged for 15 hours in water, 0.02 mol dm-3 phosphate buffer solution (pH 5.0) and 0.02 mol dm-3 phosphate buffer solution (pH 7.0) at 25 and 40ºC, respectively. The content and activity of water in the kernels before and after the pre-germination treatments were measured by oven drying and dew point water analysis, respectively. The total phenolic and phytic acid contents of the kernels were quantified by using Folin-Ciocalteu and a published spectrophotometric assay, respectively. (±)-Catechin and quercetin contents in the almond kernels were determined using gas-chromatography mass spectrometry. The total antioxidant capacity of the kernels were measured by 2,2’-diphenyl-1- picrylhydrazyl assay. Treatment with water, PBS pH 5 and PBS pH 7 significantly increased the water, total phenolic, (±)-catechin contents and total antioxidant capacity of the almond kernels regardless of the treatment temperatures (25 or 40°C). The phytic acid and quercetin contents were significantly elevated after the three treatments at 40°C. The total phenolic, (±)-catechin, quercetin and phytate contents in the almond kernels contributed significantly to its antioxidant property. Our results suggested that the phytochemical compositions of the almond kernels changed during pre-germination. The temperature and pH of the medium exert differential influence on the phytochemical compositions of the pre-germinated almond kernels. پرونده مقاله