فهرست مقالات Orchidaceae

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  1 - Micropropagation of Phalaenopsis circus via direct organogenesis using protocorm-like bodies explant
  Hasan Kiaheirati Davood Hashemabadi Behzad Kaviani
  10.30495/ijpp.2023.701446
  Phalaenopsis orchid has high economic value in the floriculture industry and is one of the most popular orchids in the world. Tissue culture techniques makes it possible to propagate and conserve this species. The aim of the present study was to evaluate the effect of d چکیده کامل

  Phalaenopsis orchid has high economic value in the floriculture industry and is one of the most popular orchids in the world. Tissue culture techniques makes it possible to propagate and conserve this species. The aim of the present study was to evaluate the effect of different concentrations of two plant growth regulators (PGRs), namely N-Phenyl-N′-1,2,3-thiadiazol-5-yl-urea (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D), alone and in combination with each other, on the number of protocorm-like bodies (PLBs), leaves, and roots, along with leaf and root lengths of micropropagated Phalaenopsis circus using leaf explants through organogenesis method. Also, correlations between these traits in the Phalaenopsis circus plantlets under study were calculated. Plantlets produced from PLBs were cultured on MS (Murashige and Skoog) basal media enriched with various levels and combinations of TDZ and 2,4-D. The optimal concentrations of the PGRs for micropropagation of Phalaenopsis circus are reported and discussed. The maximum number of PLBs (75.00) was obtained on the medium enriched with 1.00 mg l–1 2,4-D. The highest length of leaf (5.23 cm) was calculated on the medium enriched with 2.00 mg l–1 2,4-D. Concentration of 1.00 mg l–1 TDZ induced the maximum number (6.07) of leaf. The longest root length (4.15 cm) and the largest number of root (4.93) was obtained in medium augmented with 2.00 mg l–1 2,4-D. پرونده مقاله
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  2 - <i>In Vitro </i>Propagation of <i>Cephalanthera rubra</i> (L.) Rich., an Endangered Orchid, Using 2,4-D, NAA and BA
  Mahdi Zargar Azad Behzad Kaviani Shahram Sedaghathoor
  Orchids are universally popular due to the wide range of colors, sizes, shapes, and scents in their flowers. The demand for orchids as cut flowers and pot plants is increasing in the flower market. Seed germination of orchids is comparatively low in the wild as they typ چکیده کامل

  Orchids are universally popular due to the wide range of colors, sizes, shapes, and scents in their flowers. The demand for orchids as cut flowers and pot plants is increasing in the flower market. Seed germination of orchids is comparatively low in the wild as they typically require fungal symbionts during their germination. Micropropagation of orchids is a major approach to orchid conservation and commercial production. Cephalanthera rubra (L.) Rich., one of Iran&rsquo;s endangered orchid species, has been cloned using leaf as explant, Murashige and Skoog (MS) as culture medium, and &alpha;-naphthalene acetic acid (NAA) and 2,4-dichlorophenoxyacetic acid (2,4-D) (both at the concentrations of 0, 0.1, 0.2, 0.3 and 0.4 mg L&ndash;1) as well 6-benzyladenine (BA) (at 0, 1, 2, 3 and 4 mg L&ndash;1) as plant growth regulators (PGRs). In order to initiate an axenic culture, the disinfection of leaf explants was performed with sodium hypochlorite and mercury chloride. The highest number of shoots (4.33) was obtained in medium enriched with 4 mg L&ndash;1 BA. Maximum stem length (4.73 cm), leaf number (5.33) and node number (2.86) was obtained in medium supplemented with 3 mg L&ndash;1 BA. The largest number of root (5) and the highest length of root (4.83 cm) was produced on medium augmented with 0.3 mg L&ndash;1 NAA. Rooted plantlets were transferred to pots filled with peat and perlite in 1:1 proportion and acclimatized to ambient greenhouse conditions with an average of 90% survival rate. This is the first report on the micropropagation of C. rubra (L.) Rich. پرونده مقاله
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  3 - The Effect of Pollination Time and Gibberellic Acid (GA3) on the Production and Seed Germination of Phalaenopsis Orchids
  Hassan Kia Heirati Rasoul Onsinejad Fattaneh Yari
  The germination power of orchids (Orchidaceae family) seems to betoo weak due lack of albumen. The study carried out with various treatments including pollination time and GA3 for breaking dormancy and increasing seed germination of orchids. The effect of pollination ti چکیده کامل

  The germination power of orchids (Orchidaceae family) seems to betoo weak due lack of albumen. The study carried out with various treatments including pollination time and GA3 for breaking dormancy and increasing seed germination of orchids. The effect of pollination time (8 periods from January to August) and gibberellic acid (0, 500, 1000 and 1500 mg L-1) were studied on germination of Phalaenopsis orchids. Capsules containing seeds with 2, 4, 8 and 10% hypochlorite sodium were disinfected. In order to grow seedlings the culture medium of cocopeat and coal with the ratio of 1:5, and cocopeat, coal, industrial shell, and polystyrene with the ratio of 1:1:2:4 was used. Results indicated that the most appropriate concentration of sodium hypochlorite in order to disinfect the capsules was 2%. The best month for pollination of flowers was January. The highest yield from one capsule obtained 15.3 seedlings in the medium of 1/2 MS containing 1000 mg L-1 gibberellic acid. The produced seedlings were transferred to greenhouse in order to hardening. The highest rate of viability was obtained through the medium of cocopeat, coal, industrial shell, and polystyrene particles. پرونده مقاله